| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Cabrera, J., et al.
(författare)
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Transiting exoplanets from the CoRoT space mission: XXVII. CoRoT-28b, a planet orbiting an evolved star, and CoRoT-29b, a planet showing an asymmetric transit
- 2015
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Ingår i: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 579
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Tidskriftsartikel (refereegranskat)abstract
- © ESO, 2015. Context. We present the discovery of two transiting extrasolar planets by the satellite CoRoT. Aims. We aim at a characterization of the planetary bulk parameters, which allow us to further investigate the formation and evolution of the planetary systems and the main properties of the host stars. Methods. We used the transit light curve to characterize the planetary parameters relative to the stellar parameters. The analysis of HARPS spectra established the planetary nature of the detections, providing their masses. Further photometric and spectroscopic ground-based observations provided stellar parameters (log g, Teff, vsini) to characterize the host stars. Our model takes the geometry of the transit to constrain the stellar density into account, which when linked to stellar evolutionary models, determines the bulk parameters of the star. Because of the asymmetric shape of the light curve of one of the planets, we had to include the possibility in our model that the stellar surface was not strictly spherical. Results. We present the planetary parameters of CoRoT-28b, a Jupiter-sized planet (mass 0.484 ± 0.087 MJup; radius 0.955 ± 0.066 RJup) orbiting an evolved star with an orbital period of 5.208 51 ± 0.000 38 days, and CoRoT-29b, another Jupiter-sized planet (mass 0.85 ± 0.20 MJup; radius 0.90 ± 0.16 RJup) orbiting an oblate star with an orbital period of 2.850 570 ± 0.000 006 days. The reason behind the asymmetry of the transit shape is not understood at this point. Conclusions. These two new planetary systems have very interesting properties and deserve further study, particularly in the case of the star CoRoT-29.
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| 3. |
- Grziwa, S., et al.
(författare)
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K2-31B, a Grazing Transiting Hot Jupiter on a 1.26-Day Orbit Around a Bright G7v Star
- 2016
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Ingår i: Astronomical Journal. - : American Astronomical Society. - 1538-3881 .- 0004-6256. ; 152:5
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Tidskriftsartikel (refereegranskat)abstract
- We report the discovery of K2-31b, the first confirmed transiting hot Jupiter detected by the K2 space mission. We combined K2 photometry with FastCam lucky imaging and FIES and HARPS high-resolution spectroscopy to confirm the planetary nature of the transiting object and derived the system parameters. K2-31b is a 1.8-Jupiter-mass planet on a. 1.26-day orbit around a G7 V star (M-star = 0.91 M-circle dot, R-star = 0.78 R-circle dot). The planetary radius is poorly constrained (0.7
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| 4. |
- Guenther, E. W., et al.
(författare)
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A new mass and radius determination of the ultra-short period planet K2-106b and the fluffy planet K2-106c
- 2024
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Ingår i: Monthly Notices of the Royal Astronomical Society. - 0035-8711 .- 1365-2966. ; 529:1, s. 141-154
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Tidskriftsartikel (refereegranskat)abstract
- Ultra-short period planets (USPs) have orbital periods of less than 1 d. Since their masses and radii can be determined to a higher precision than long-period planets, they are the preferred targets to determine the density of planets which constrains their composition. The K2-106 system is particularly interesting because it contains two planets of nearly identical masses. One is a high-density USP, the other is a low-density planet that has an orbital period of 13 d. Combining the Gaia DR3 results with new ESPRESSO data allows us to determine the masses and radii of the two planets more precisely than before. We find that the USP K2-106 b has a density consistent with an Earth-like composition, and K2-106 c is a low-density planet that presumably has an extended atmosphere. We measure a radius of R p = 1 . 676 + 0 . 037 -0 . 037 R ⊕, a mass of M p = 7 . 80 + 0 . 71 -0 . 70 M ⊕, and a density of ρ= 9 . 09 + 0 . 98 -0 . 98 g cm -3 for K2-106 b. For K2-106 c, we derive R p = 2 . 84 + 0 . 10 -0 . 08 R ⊕, M p = 7 . 3 + 2 . 5 -2 . 4 M ⊕, and a density of ρ= 1 . 72 + 0 . 66 -0 . 58 g cm -3 . We finally discuss the possible structures of the two planets with respect to other low-mass planets.
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| 5. |
- Guenther, L., et al.
(författare)
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Impact of fixed-dose combination Cal/BD foam on the work productivity of patients with psoriasis: results from the 52-week randomized, double-blind, PSO-LONG trial
- 2022
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Ingår i: Journal of the European Academy of Dermatology and Venereology. - : WILEY. - 0926-9959 .- 1468-3083. ; 36:7, s. 1054-1063
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Tidskriftsartikel (refereegranskat)abstract
- Background Psoriasis contributes to unemployment, work impairment, missed workdays and substantial indirect costs due to lost productivity. Combination Cal/BD foam is the only topical that is approved for long-term maintenance treatment of plaque psoriasis for 52 weeks. This is the first known investigation of the effect of topical psoriasis therapy on productivity. Objective To examine the change in work productivity and activity impairment after 4 weeks of treatment with fixed-dose combination calcipotriol 50 mu g/g/betamethasone dipropionate 0.5 mg/g (Cal/BD) foam and observe long-term changes after 52 weeks of long-term management (proactive or reactive treatment). Methods This is a post-hoc analysis of the PSO-LONG trial - a phase 3, randomized, double-blind, vehicle-controlled, parallel group, international multi-centre trial of treatment with combination Cal/BD foam. Work and activity impairment due to psoriasis were assessed by the Dermatology Life Quality Index (DLQI) and the Work Productivity and Activity Impairment Psoriasis (WPAI:PSO) questionnaire at baseline, week 4, week 28 and week 56. The improvement in hours of work productivity was translated into monthly and annual indirect cost savings estimates for patients in Italy, Sweden, United Kingdom, Canada and Germany. Results Using fixed-dose combination Cal/BD foam for four weeks significantly reduced psoriasis-related work presenteeism, total work productivity impairment (TWPI) and total activity impairment (TAI) over 56 weeks, with significant improvements observed as early as 4 weeks after the baseline visit. The proportion of patients reporting impact on work productivity (as measured by presenteeism and TWPI) and activity impairment (as measured by both DLQI-Q7b and TAI) also decreased. Conclusion Fixed-dose combination Cal/BD foam used for long-term management of psoriasis significantly reduces psoriasis-related work productivity and activity impairment which may result in substantial indirect cost savings.
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| 6. |
- Hermann, Sebastian, 1977-, et al.
(författare)
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In The CLEW Model – Developing an integrated tool for modelling the interrelated effects of Climate, Land use, Energy, and Water (CLEW)
- 2011
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Ingår i: 6th Dubrovnik Conference on Sustainable Development of Energy, Water and Environment Systems - Proceedings.
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Konferensbidrag (refereegranskat)abstract
- This paper introduces the prototype of new tool which analyses the Climate-, Land-, Energy- and Water- (CLEW) re-sources and their interactions and implications associated with socio-economic development. The presented CLEW model focuses specifically on the analysis of different energy (technology) options and their impact on other resources – e.g. their contribution to climate change, land use change, and water consumption.The CLEW model systematically quantifies trade-offs associated with actions aimed at meeting development goals (specifically energy, food, and water supply) and their impact on the climate, water and environment. The model quantifies resource use with calculations based on collected data, assumptions and user-defined scenarios. Importantly, the model is not limited to internal or national effects but also includes external changes induced through energy imports or exports and land use change. Exemplary, a first preliminary modelling exercise for the island of Mauritius has shown very strong implications on GHG emissions when switching to locally produced biofuels (bio-ethanol) through induced land-use changes and is presented in the second part of this paper.
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| 7. |
- Howells, Mark I., et al.
(författare)
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Integrated analysis of climate change, land-use, energy and water strategies
- 2013
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Ingår i: Nature Climate Change. - : Springer Nature. - 1758-678X .- 1758-6798. ; 3:7, s. 621-626
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Tidskriftsartikel (refereegranskat)abstract
- Land, energy and water are our most precious resources, but the manner and extent to which they are exploited contributes to climate change. Meanwhile, the systems that provide these resources are themselves highly vulnerable to changes in climate. Efficient resource management is therefore of great importance, both for mitigation and for adaptation purposes. We postulate that the lack of integration in resource assessments and policy-making leads to inconsistent strategies and inefficient use of resources. We present CLEWs (climate, land-use, energy and water strategies), a new paradigm for resource assessments that we believe can help to remedy some of these shortcomings.
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| 8. |
- Jost, Stefanie T., et al.
(författare)
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Levodopa Dose Equivalency in Parkinson's Disease : Updated Systematic Review and Proposals
- 2023
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Ingår i: Movement Disorders. - 0885-3185. ; 38:7, s. 1236-1252
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Tidskriftsartikel (refereegranskat)abstract
- Background: To compare drug regimens across clinical trials in Parkinson's disease (PD) conversion formulae between antiparkinsonian drugs have been developed. These are reported in relation to levodopa as the benchmark drug in PD pharmacotherapy as ‘levodopa equivalent dose’ (LED). Currently, the LED conversion formulae proposed in 2010 by Tomlinson et al. based on a systematic review are predominantly used. However, new drugs with established and novel mechanisms of action and novel formulations of longstanding drugs have been developed since 2010. Therefore, consensus proposals for updated LED conversion formulae are needed. Objectives: To update LED conversion formulae based on a systematic review. Methods: The MEDLINE, CENTRAL, and Embase databases were searched from January 2010 to July 2021. Additionally, in a standardized process according to the GRADE grid method, consensus proposals were issued for drugs with scarce data on levodopa dose equivalency. Results: The systematic database search yielded 3076 articles of which 682 were eligible for inclusion in the systematic review. Based on these data and the standardized consensus process, we present proposals for LED conversion formulae for a wide range of drugs that are currently available for the pharmacotherapy of PD or are expected to be introduced soon. Conclusions: The LED conversion formulae issued in this Position Paper will serve as a research tool to compare the equivalence of antiparkinsonian medication across PD study cohorts and facilitate research on the clinical efficacy of pharmacological and surgical treatments as well as other non-pharmacological interventions in PD.
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| 9. |
- Lieske, Julia, et al.
(författare)
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On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies
- 2019
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Ingår i: IUCrJ. - 2052-2525. ; 6:4, s. 714-728
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Tidskriftsartikel (refereegranskat)abstract
- Efficient and reliable sample delivery has remained one of the bottlenecks for serial crystallography experiments. Compared with other methods, fixed-target sample delivery offers the advantage of significantly reduced sample consumption and shorter data collection times owing to higher hit rates. Here, a new method of on-chip crystallization is reported which allows the efficient and reproducible growth of large numbers of protein crystals directly on micro-patterned silicon chips for in-situ serial crystallography experiments. Crystals are grown by sitting-drop vapor diffusion and previously established crystallization conditions can be directly applied. By reducing the number of crystal-handling steps, the method is particularly well suited for sensitive crystal systems. Excessive mother liquor can be efficiently removed from the crystals by blotting, and no sealing of the fixed-target sample holders is required to prevent the crystals from dehydrating. As a consequence, 'naked' crystals are obtained on the chip, resulting in very low background scattering levels and making the crystals highly accessible for external manipulation such as the application of ligand solutions. Serial diffraction experiments carried out at cryogenic temperatures at a synchrotron and at room temperature at an X-ray free-electron laser yielded high-quality X-ray structures of the human membrane protein aquaporin 2 and two new ligand-bound structures of thermolysin and the human kinase DRAK2. The results highlight the applicability of the method for future high-throughput on-chip screening of pharmaceutical compounds.
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| 10. |
- Nespral, D., et al.
(författare)
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Mass determination of K2-19b and K2-19c from radial velocities and transit timing variations
- 2017
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Ingår i: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 601, s. Article Number: A128-
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Tidskriftsartikel (refereegranskat)abstract
- We present radial velocity follow-up observations of K2-19, a compact planetary system hosting three planets, of which the two larger ones, K2-19b and K2-19c, are close to the 3: 2 mean motion resonance. An analysis considering only the radial velocity measurements detects K2-19b, the larger and more massive planet in the system, with a mass of 54.8 +/- 7.5 M-circle plus and provides a marginal detection of K2-19c, with a mass of M-c = 5.9(-4.3)(+7.6) M-circle plus. We also used the TRADES code to simultaneously model both our RV measurements and the existing transit timing measurements. We derived a mass of 54.4 +/- 8.9 M-circle plus for K2-19b and of 7.5(-1.4)(+3.0) M-circle plus for K2-19c. For K2-19b, these masses are consistent with a previous determination that was principally based on a photodynamical analysis of the K2-19 light curve. Differences remain mainly in the mass determination of the more lightweight planet, driven likely by the limited precision of the RV measurements and possibly some as yet unrecognized systematics.
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