| 1. |
- Li, Hanbing, et al.
(författare)
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Dual Function of PI(4,5)P2 in Insulin-Regulated Exocytic Trafficking of GLUT4 in Adipocytes
- 2020
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Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836 .- 1089-8638. ; 432:16, s. 4341-4357
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Tidskriftsartikel (refereegranskat)abstract
- Phosphoinositides are important signaling molecules involved in the regulation of vesicular trafficking. It has been implicated that phosphatidylinositol 4,5-bisphosphate [PI(4,5)P-2] is involved in insulin-regulated GLUT4 translocation in adipocytes. However, it remains unclear where and how PI(4,5)P-2 regulates discrete steps of GLUT4 vesicle translocation in adipocytes, especially on the exocytic arm of regulation. Here, we employed optogenetic tools to acutely control the PI(4,5)P-2 metabolism in living cells. By combination of TIRFM imaging, we were able to monitor the temporal-spatial-dependent PI(4,5)P-2 regulation on discrete steps of GLUT4 translocation in adipocytes. We found that the plasma membrane localized PI(4,5)P-2 is crucial for proper insulin signaling propagation and for insulin-stimulated GLUT4 vesicle translocation in 3T3-L1 adipocytes. Global depletion of PI(4,5)P-2 on the cell surface blunted insulin-stimulated Akt phosphorylation and abolished insulin effects in promotion of the docking and fusion of GLUT4 vesicle with the plasma membrane. Furthermore, by development of a novel optogenetic module to selectively modulate PI(4,5)P-2 levels on the GLUT4 vesicle docking site, we identified an important regulatory role of PI(4,5)P-2 in controlling of vesicle docking process. Local depletion of PI(4,5)P-2 at the vesicle docking site promoted GLUT4 vesicle undocking, diminished insulin-stimulated GLUT4 vesicle docking and fusion, but without perturbation of insulin signaling propagation in adipocytes. Our results provide strong evidence that cell surface PI(4,5)P-2 plays two distinct functions on regulation of the exocytic trafficking of GLUT4 in adipocytes. PI(4,5)P-2 not only regulates the proper activation of insulin signaling in general but also controls GLUT4 vesicle docking process at the vesicle-membrane contact sites.
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| 2. |
- Liu, Juzhe, et al.
(författare)
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Ultrathin amorphous cobalt-vanadium hydr(oxy)oxide catalysts for the oxygen evolution reaction
- 2018
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Ingår i: Energy & Environmental Science. - : Royal Society of Chemistry. - 1754-5692 .- 1754-5706. ; 11:7, s. 1736-1741
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Tidskriftsartikel (refereegranskat)abstract
- Cost efficient and long-term stable catalysts are in great demand for the oxygen evolution reaction (OER), a key process involved in water splitting cells and metal-air batteries. Here, we demonstrate that the ultrathin amorphous cobalt-vanadium hydr(oxy)oxide we synthesized is a highly promising electrocatalytic material for the OER with a low overpotential of 0.250 V (even lower down to 0.215 V when supported on Au foam) at 10 mA cm(-2) and a long stable operation time (170 h) in alkaline media. In combination with in situ X-ray absorption spectral characterization and first-principles simulations, we reveal that the ultrathin, amorphous and alloyed structural characteristics have enabled its facile transformation to the desirable active phase, leading to a dramatically enhanced catalytic activity. Our finding highlights the remarkable advantages of the two-dimensional amorphous material and sheds new light on the design of high-performance electrocatalysts.
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| 3. |
- Zhao, Xiaogang, et al.
(författare)
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Safety Prediction of Soleplate Corrosion State in Petroleum Storage Tank Based on Grey Theory Model
- 2016
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Ingår i: 3rd International Conference on Applied Engineering. - : AIDIC - associazione italiana di ingegneria chimica. - 9788895608433 ; , s. 271-276
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Konferensbidrag (refereegranskat)abstract
- The corrosion protection of petroleum storage tank is important for safety work in petroleum depot. Precise prediction of soleplate corrosion may reflect the security state of a storage tank, and it can provide scientific references to the anti-corrosion and maintenance in safety management. Grey Model GM (1,1) in the Grey Theory is a good prediction method based on small volume of original data, and it is introduced and applied to predict the soleplate corrosion of No. G-2 petroleum storage tank in Luquan oil depot. In order to improving the calculation speed, the GM (1,1) model is programmed by MATLAB and is further utilized in data processing. Compared with the measurements, the prediction results meet them very well, which shows that this model is practical. Meanwhile, the prediction results also reflect the status and the corrosion rate of a petroleum tank which is of great importance for the future safety management and maintenance works. Meanwhile, the GM (1,1) is suitable for wide applications in other relative fields in oil depots.
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