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- Di Bartolomeo, Francesca, 1986, et al.
(författare)
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Absolute yeast mitochondrial proteome quantification reveals trade-off between biosynthesis and energy generation during diauxic shift
- 2020
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 117:13, s. 7524-7535
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Tidskriftsartikel (refereegranskat)abstract
- Saccharomyces cerevisiae constitutes a popular eukaryal model for research on mitochondrial physiology. Being Crabtree-positive, this yeast has evolved the ability to ferment glucose to ethanol and respire ethanol once glucose is consumed. Its transition phase from fermentative to respiratory metabolism, known as the diauxic shift, is reflected by dramatic rearrangements of mitochondrial function and structure. To date, the metabolic adaptations that occur during the diauxic shift have not been fully characterized at the organelle level. In this study, the absolute proteome of mitochondria was quantified alongside precise parametrization of biophysical properties associated with the mitochondrial network using state-of-the-art optical-imaging techniques. This allowed the determination of absolute protein abundances at a subcellular level. By tracking the transformation of mitochondrial mass and volume, alongside changes in the absolute mitochondrial proteome allocation, we could quantify how mitochondria balance their dual role as a biosynthetic hub as well as a center for cellular respiration. Furthermore, our findings suggest that in the transition from a fermentative to a respiratory metabolism, the diauxic shift represents the stage where major structural and functional reorganizations in mitochondrial metabolism occur. This metabolic transition, initiated at the mitochondria level, is then extended to the rest of the yeast cell.
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| 2. |
- Echeverri, Per, 1958-, et al.
(författare)
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Consumers’ Experience Rooms : Environmental Design Factors in Shopping Processes
- 2009
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Shopping has become one of our most common leisure pursuits. Consumers move freely between stores and their different environments, often in a random manner. The question is how much randomness there actually is regarding how these environments make the customers feel and move. We have chosen to observe consumers in order to see how the environment affects their perceptions. The practical aim of this study is to deepen our understanding of how consumers are affected by the service environment of a shopping centre and based on that identify improvements as regards to design. The theoretical aim is to identify what environmental dimensions appear to be important in a real-time purchase situation. This includes a validation of some theories of service environment dimensions and design attributes. The methodological aim is to further elaborate on video-supported field observation. Empirical material is gathered at a shopping centre in Karlstad, Sweden, called Mitt i City. Video and audio recordings together with personal interviews in situ are used in documenting consumption processes, patterns of movements and perceptions. We observed and interviewed our informants before, during, and after their visits to the shopping centre. These aims lead to a clarification as regards to how the service environment creates an experience, meaning and function for the consumers by means of the concept of the shopping centre. The study shows that design factors (such as layout, spatial form, colour combinations, choices of material and character), together with atmospherics (sound, light, temperature) and furnishing, are important parameters for consumers in their sensemaking of a shopping concept and the functionality of the shopping procedure per se. Contact information For further information on authors and details of the study, please contact Per Echeverri at Service Research Center – CTF, Faculty of Economy, Communication and IT, Karlstad University. E-mail: per.echeverri@kau.se
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