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- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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- Crocetti, Elisabetta, et al.
(författare)
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Generalized Anxiety Symptoms and Identity Processes in Cross-Cultural Samples of Adolescents from the General Population
- 2015
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Ingår i: Child and Youth Care Forum. - : Springer Science and Business Media LLC. - 1053-1890 .- 1573-3319. ; 44:2, s. 159-174
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Tidskriftsartikel (refereegranskat)abstract
- Approximately 20 % of adolescents around the world experience mental health problems, most commonly depression or anxiety. High levels of anxiety disorder symptoms can hinder adolescent development, persist into adulthood, and predict negative mental outcomes, such as suicidal ideation and attempts. We analyzed generalized anxiety disorder (GAD) symptoms in cross-cultural samples from the general population. We sought to examine cultural and gender differences, and correlates of GAD symptoms in samples of adolescents from six countries located in three different continents (Europe: Bulgaria, Italy, the Netherlands; Africa: Kenya; Asia: China and Philippines). Participants were 3,445 (51 % male) adolescents aged between 14 and 18 years old. They filled self-report measures of GAD symptoms and identity. First, it was found that the scores on GAD symptoms varied significantly across countries, with Dutch respondents reporting the lowest levels whereas Filipino participants exhibited the highest levels of GAD symptoms. Second, gender differences (i.e., girls reported more GAD symptoms than boys) were significant in each country (as well as in the total sample), with the only exception being that of Kenya. Third, GAD symptoms were significantly related to identity processes and similarities and differences across countries were examined. This study highlighted that prevalence, gender differences, and correlates of GAD vary across countries. Therefore, it is important when researching GAD symptoms to examine one's research findings within a global perspective.
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