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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 5. |
- An, Junghwa, et al.
(författare)
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Permanent Genetic Resources added to Molecular Ecology Resources Database 1 October 2009-30 November 2009
- 2010
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Ingår i: Molecular Ecology Resources. - : Wiley. - 1755-098X .- 1755-0998. ; 10:2, s. 404-408
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Tidskriftsartikel (refereegranskat)abstract
- This article documents the addition of 411 microsatellite marker loci and 15 pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Acanthopagrus schlegeli, Anopheles lesteri, Aspergillus clavatus, Aspergillus flavus, Aspergillus fumigatus, Aspergillus oryzae, Aspergillus terreus, Branchiostoma japonicum, Branchiostoma belcheri, Colias behrii, Coryphopterus personatus, Cynogolssus semilaevis, Cynoglossus semilaevis, Dendrobium officinale, Dendrobium officinale, Dysoxylum malabaricum, Metrioptera roeselii, Myrmeciza exsul, Ochotona thibetana, Neosartorya fischeri, Nothofagus pumilio, Onychodactylus fischeri, Phoenicopterus roseus, Salvia officinalis L., Scylla paramamosain, Silene latifo, Sula sula, and Vulpes vulpes. These loci were cross-tested on the following species: Aspergillus giganteus, Colias pelidne, Colias interior, Colias meadii, Colias eurytheme, Coryphopterus lipernes, Coryphopterus glaucofrenum, Coryphopterus eidolon, Gnatholepis thompsoni, Elacatinus evelynae, Dendrobium loddigesii Dendrobium devonianum, Dysoxylum binectariferum, Nothofagus antarctica, Nothofagus dombeyii, Nothofagus nervosa, Nothofagus obliqua, Sula nebouxii, and Sula variegata. This article also documents the addition of 39 sequencing primer pairs and 15 allele specific primers or probes for Paralithodes camtschaticus.
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| 6. |
- Chen, Lan, et al.
(författare)
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The impacts of building height variations and building packing densities on flow adjustment and city breathability in idealized urban models
- 2017
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Ingår i: Building and Environment. - : Elsevier BV. - 0360-1323 .- 1873-684X. ; 118, s. 344-361
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Tidskriftsartikel (refereegranskat)abstract
- Improving city breathability has been confirmed as one feasible measure to improve pollutant dilution in the urban canopy layer (UCL). Building height variability enhances vertical mixing, but its impacts remain not completely explored. Therefore, both wind tunnel experiments and computational fluid dynamic (CFD) simulations are used to investigate the effect of building height variations (six height standard deviations σH = 0%–77.8%) associated to building packing densities namely λp/λf = 0.25/0.375 (medium-density) and 0.44/0.67 (compact) on city breathability. Two bulk variables (i.e. the in-canopy velocity (UC) and exchange velocity (UE)) are adopted to quantify the horizontal and vertical city breathability respectively, which are normalized by the reference velocity (Uref) in the free flow, typically set at z = 2.5H0 where H0 is the mean building height. Both flow quantities and city breathability experience a flow adjustment process, then reach a balance. The adjustment distance is at least three times longer than four rows documented in previous literature. The medium-density arrays experience much larger UC and UE than the compact ones. UE is found mainly induced by vertical turbulent fluxes, instead of vertical mean flows. In height-variation cases, taller buildings experience larger drag force and city breathability than lower buildings and those in uniform-height cases. For medium-density and compact models with uniform height, the balanced UC/Uref are 0.124 and 0.105 respectively, moreover the balanced UE/Uref are 0.0078 and 0.0065. In contrast, the average UC/Uref in height-variation cases are larger (115.3%–139.5% and 125.7%–141.9% of uniform-height cases) but UE/Uref are smaller (74.4%–79.5% and 61.5%–86.2% of uniform-height cases) for medium-density and compact models.
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| 7. |
- Chen, Lan, et al.
(författare)
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The Influence of Building Packing Densities on Flow Adjustment and City Breathability in Urban-like Geometries
- 2017
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Ingår i: Procedia Engineering. - : Elsevier Ltd. - 1877-7058. ; 198, s. 758-769
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Tidskriftsartikel (refereegranskat)abstract
- City breathability refers to the air exchange process between the flows above and within urban canopy layers (UCL) and that of in-canopy flow, measuring the potential of wind to remove and dilute pollutants, heat and other scalars in a city. Bulk flow parameters such as in-canopy velocity (Uc) and exchange velocity (UE) have been applied to evaluate the city breathability. Both wind tunnel experiments and computational fluid dynamics (CFD) simulations were used to study the flow adjustment and the variation of city breathability through urban-like models with different building packing densities. We experimentally studied some 25-row and 15-column aligned cubic building arrays (the building width B=72 mm and building heights H=B) in a closed-circuit boundary layer wind tunnel. Effect of building packing densities (λp=λf=0.11, 0.25, 0.44) on flow adjustment and drag force of each buildings were measured. Wind tunnel data show that wind speed decreases quickly through building arrays due to strong building drag. The first upstream building induces the strongest flow resistance. The flow adjustment length varies slightly with building packing densities. Larger building packing density produces lower drag force by individual buildings and attains smaller velocity in urban canopy layers, which causes weaker city breathability capacity. In CFD simulations, we performed seven test cases with various building packing densities of λp=λf=0.0625, 0.11, 0.25, 0.36, 0.44 and 0.56. In the cases of λp=λf=0.11, 0.25, 0.44, the simulated profiles of velocity and drag force agree with experiment data well. We computed Uc and UE, which represent horizontal and vertical ventilation capacity respectively. The inlet velocity at 2.5 times building height in the upstream free flow is defined as the reference velocity Uref. Results show that UE/Uref changes slightly (1.1% to 0.7%) but Uc/Uref significantly decreases from 0.4 to 0.1 as building packing densities rise from 0.0625 to 0.56. Although UE is induced by both mean flows and turbulent momentum flux across the top surface of urban canopy, vertical turbulent diffusion is found to contribute mostly to UE.
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| 8. |
- Fan, Jin, et al.
(författare)
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Development of wideband orthomode transducers for FAST cryogenic receiver system
- 2020
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Ingår i: Research in Astronomy and Astrophysics. - : IOP Publishing. - 1674-4527. ; 20:5
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Tidskriftsartikel (refereegranskat)abstract
- This paper describes the design, construction, and performance of the wideband orthomode transducers (OMTs) for the L- (1.2-1.8 GHz), the S- (2-3 GHz) and the P- (0.56-1.12 GHz) band receiver systems of the Five-hundred-meter Aperture Spherical radio Telescope (FAST). These OMTs operate at the cryogenic temperature of 70K to reduce their thermal noise contribution to the receiver chains. The development on the FAST L- and S-band quad-ridged waveguide (QRWG) OMTs is carried out based on the theoretical mode analysis. In view of the miniaturization of FAST cryogenic receiver system at P-band, a novel wideband compact bowtie dipole OMT is designed with an octave bandwidth as well as a length of only quarter wavelength. The proposed L-, S- and P-band OMTs are designed and optimized by using Ansys High Frequency Structure Simulator (HFSS), and then manufactured, tested at room temperature. Measurement of FAST cryogenic receiver system noise is also performed with the L-, S- and P-band OMTs installed. The measured results fully comply with the design specifications.
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| 9. |
- Hang, Jian, et al.
(författare)
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Age of air and air exchange efficiency in idealized city models
- 2009
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Ingår i: Building and Environment. - : Elsevier BV. - 0360-1323 .- 1873-684X. ; 44:8, s. 1714-1723
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Tidskriftsartikel (refereegranskat)abstract
- Wind can provide relevantly clean external (rural) air into urban street network, i.e. city ventilation. The local mean age of air denotes the time it takes for the external air to reach a location after entering the urban canopy layer. The air exchange efficiency denotes the efficiency of flushing the street network with external air. However, difficulties exist in calculating the local mean age of air in a city due to open boundaries. The traditional experimental homogeneous emission method is adapted here in a CFD method to predict the urban local age of air and analyze the air exchange efficiency for city ventilation. Three simple city models are considered, including a round city model, a square city model and a long rectangular city with one main street parallel to the approaching wind or with two crossing streets. The difference in the city shape results in significant difference in the local mean age of air. In the round city of one narrow street, two inflows through street openings converge close to the city centre and exits through the street roof, so the air close to the city centre is relatively old and the air exchange efficiency is low (30%). For a round city with two crossing streets, a slightly non-parallel wind to the main street generates younger air and the higher air exchange efficiency in the city.
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| 10. |
- Hang, Jian, et al.
(författare)
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City breathability in medium density urban-like geometries evaluated through the pollutant transport rate and the net escape velocity
- 2015
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Ingår i: Building and Environment. - : Elsevier BV. - 0360-1323 .- 1873-684X. ; 94:P1, s. 166-182
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Tidskriftsartikel (refereegranskat)abstract
- This paper investigates pollutant removal at pedestrian level in urban canopy layer (UCL) models of medium packing density (λp = λf = 0.25) using computational fluid dynamics (CFD) simulations. Urban size, building height variations, wind direction and uniform wall heating are investigated. The standard and RNG k-ε turbulence models, validated against wind tunnel data, are used. The contribution of mean flows and turbulent diffusion in removing pollutants at pedestrian level is quantified by three indicators: the net escape velocity (NEV), the pollutant transport rate (PTR) across UCL boundaries and their contribution ratios (CR).Results show that under parallel approaching wind, after a wind-adjustment region, a fully-developed region develops. Longer urban models attain smaller NEV due to pollutant accumulation. Specifically, for street-scale models (~100 m), most pollutants are removed out across leeward street openings and the dilution by horizontal mean flows contributes mostly to NEV. For neighbourhood-scale models (~1 km), both horizontal mean flows and turbulent diffusion contribute more to NEV than vertical mean flows which instead produce significant pollutant re-entry across street roofs. In contrast to uniform height, building height variations increase the contribution of vertical mean flows, but only slightly influence NEV. Finally, flow conditions with parallel wind and uniform wall heating attain larger NEV than oblique wind and isothermal condition.The paper proves that by analysing the values of the three indicators it is possible to form maps of urban breathability according to prevailing wind conditions and known urban morphology that can be of easy use for planning purposes.
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