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- Spjut, Sara, 1978-, et al.
(författare)
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Carbamate linker strategy in solid-phase synthesis of amino-functionalized glycoconjugates for attachment to solid surfaces and investigation of protein-carbohydrate interactions
- 2009
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Ingår i: European Journal of Organic Chemistry. - Weinheim : WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. - 1434-193X .- 1099-0690. ; :3, s. 349-57
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Tidskriftsartikel (refereegranskat)abstract
- Amino-functionalized serine-based galactose and glucose neoglycolipids were prepared by solid-phase synthesis using a carbamate strategy for anchoring amino functionalities to a (2-fluoro-4-hydroxymethylphenoxy)acetic acid linker resin. Key synthetic steps were monitored with gel-phase 19F NMR spectroscopy. Cleavage from the solid support was performed with trifluoroacetic acid. The terminal amine of the neoglycolipids was conjugated with didecyl squarate and then immobilized in amino-functionalized microtiter plates and the glycoconjugates were successfully probed with a galactose-binding lectin.
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