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- Hayes, Sally, et al.
(författare)
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The structural and optical properties of type III human collagen biosynthetic corneal substitutes
- 2015
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Ingår i: Acta Biomaterialia. - : ELSEVIER SCI LTD. - 1742-7061 .- 1878-7568. ; 25, s. 121-130
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Tidskriftsartikel (refereegranskat)abstract
- The structural and optical properties of clinically biocompatible, cell-free hydrogels comprised of synthetically cross-linked and moulded recombinant human collagen type III (RHCIII) with and without the incorporation of 2-methacryloyloxyethyl phosphorylcholine (MPC) were assessed using transmission electron microscopy (TEM), X-ray scattering, spectroscopy and refractometry. These findings were examined alongside similarly obtained data from 21 human donor corneas. TEM demonstrated the presence of loosely bundled aggregates of fine collagen filaments within both RHCIII and RHCIII-MPC implants, which X-ray scattering showed to lack D-banding and be preferentially aligned in a uniaxial orientation throughout. This arrangement differs from the predominantly biaxial alignment of collagen fibrils that exists in the human cornea. By virtue of their high water content (90%), very fine collagen filaments (2-9 nm) and lack of cells, the collagen hydrogels were found to transmit almost all incident light in the visible spectrum. They also transmitted a large proportion of UV light compared to the cornea which acts as an effective UV filter. Patients implanted with these hydrogels should be cautious about UV exposure prior to regrowth of the epithelium and in-growth of corneal cells into the implants. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd.
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| 2. |
- Islam, Mohammad Mirazul, 1984-, et al.
(författare)
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Biomaterials-enabled cornea regeneration in patients at high risk for rejection of donor tissue transplantation
- 2018
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Ingår i: NPJ Regenerative medicine. - : Springer Science and Business Media LLC. - 2057-3995. ; 3
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Tidskriftsartikel (refereegranskat)abstract
- The severe worldwide shortage of donor organs, and severe pathologies placing patients at high risk for rejecting conventional cornea transplantation, have left many corneal blind patients untreated. Following successful pre-clinical evaluation in mini-pigs, we tested a biomaterials-enabled pro-regeneration strategy to restore corneal integrity in an open-label observational study of six patients. Cell-free corneal implants comprising recombinant human collagen and phosphorylcholine were grafted by anterior lamellar keratoplasty into corneas of unilaterally blind patients diagnosed at high-risk for rejecting donor allografts. They were followed-up for a mean of 24 months. Patients with acute disease (ulceration) were relieved of pain and discomfort within 1-2 weeks post-operation. Patients with scarred or ulcerated corneas from severe infection showed better vision improvement, followed by corneas with burns. Corneas with immune or degenerative conditions transplanted for symptom relief only showed no vision improvement overall. However, grafting promoted nerve regeneration as observed by improved touch sensitivity to near normal levels in all patients tested, even for those with little/no sensitivity before treatment. Overall, three out of six patients showed significant vision improvement. Others were sufficiently stabilized to allow follow-on surgery to restore vision. Grafting outcomes in mini-pig corneas were superior to those in human subjects, emphasizing that animal models are only predictive for patients with non-severely pathological corneas; however, for establishing parameters such as stable corneal tissue and nerve regeneration, our pig model is satisfactory. While further testing is merited, we have nevertheless shown that cell-free implants are potentially safe, efficacious options for treating high-risk patients.
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| 3. |
- Jung, Christian, et al.
(författare)
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A comparison of very old patients admitted to intensive care unit after acute versus elective surgery or intervention
- 2019
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Ingår i: Journal of critical care. - : W B SAUNDERS CO-ELSEVIER INC. - 0883-9441 .- 1557-8615. ; 52, s. 141-148
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Tidskriftsartikel (refereegranskat)abstract
- Background: We aimed to evaluate differences in outcome between patients admitted to intensive care unit (ICU) after elective versus acute surgery in a multinational cohort of very old patients (80 years; VIP). Predictors of mortality, with special emphasis on frailty, were assessed.Methods: In total, 5063 VIPs were induded in this analysis, 922 were admitted after elective surgery or intervention, 4141 acutely, with 402 after acute surgery. Differences were calculated using Mann-Whitney-U test and Wilcoxon test. Univariate and multivariable logistic regression were used to assess associations with mortality.Results: Compared patients admitted after acute surgery, patients admitted after elective surgery suffered less often from frailty as defined as CFS (28% vs 46%; p < 0.001), evidenced lower SOFA scores (4 +/- 5 vs 7 +/- 7; p < 0.001). Presence of frailty (CFS >4) was associated with significantly increased mortality both in elective surgery patients (7% vs 12%; p = 0.01), in acute surgery (7% vs 12%; p = 0.02).Conclusions: VIPs admitted to ICU after elective surgery evidenced favorable outcome over patients after acute surgery even after correction for relevant confounders. Frailty might be used to guide clinicians in risk stratification in both patients admitted after elective and acute surgery.
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| 4. |
- Saldana-Lopez, Alberto, et al.
(författare)
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The Low-Redshift Lyman Continuum Survey Unveiling the ISM properties of low-z Lyman-continuum emitters
- 2022
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Ingår i: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 663
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Tidskriftsartikel (refereegranskat)abstract
- Aims. Combining 66 ultraviolet (UV) spectra and ancillary data from the recent Low-Redshift Lyman Continuum Survey (LzLCS) and 23 LyC observations by earlier studies, we form a statistical sample of star-forming galaxies at z similar to 0.2-0.4 with which we study the role of cold interstellar medium (ISM) gas in the leakage of ionizing radiation. We also aim to establish empirical relations between the H I neutral and low-ionization state (LIS) absorption lines with different galaxy properties.Methods. We first constrain the massive star content (stellar ages and metallicities) and UV attenuation by fitting the stellar continuum with a combination of simple stellar population models. The models, together with accurate LyC flux measurements, allow us to determine the absolute LyC photon escape fraction for each galaxy (f(esc)(abs)). We then measure the equivalent widths and residual fluxes of multiple H I and LIS lines, and the geometrical covering fraction of the UV emission, adopting the picket-fence model.Results. The LyC escape fraction spans a wide range, with a median f(esc)(abs) (0.16, 0.84 quantiles) of 0.04 (0.02, 0.20), and 50 out of the 89 galaxies detected in the LyC (1 sigma upper limits of f(esc)(abs) less than or similar to 0.01 for non-detections, typically). The H I and LIS line equivalent widths scale with the UV luminosity and attenuation, and inversely with the residual flux of these lines. Additionally, Ly alpha equivalent widths scale with both the H I and LIS residual fluxes, but anti-correlate with the corresponding H I or LIS equivalent widths. The H I and LIS residual fluxes are correlated, indicating that the neutral gas is spatially traced by the low-ionization transitions. We find that the observed trends of the absorption lines and the UV attenuation are primarily driven by the geometric covering fraction of the gas. The observed nonuniform gas coverage also demonstrates that LyC photons escape through low-column-density channels in the ISM. The equivalent widths and residual fluxes of both the H I and LIS lines strongly correlate with f(esc)(abs): strong LyC leakers (highest f(esc)(abs)) show weak absorption lines, low UV attenuation, and large Ly alpha equivalent widths. We provide several empirical calibrations to estimate f(esc)(abs) from UV absorption lines. Finally, we show that simultaneous UV absorption line and dust attenuation measurements can, in general, predict the escape fraction of galaxies. We apply our method to available measurements of UV LIS lines of 15 star-forming galaxies at z similar to 4 6 (plus 3 high-z galaxy composites), finding that these high-redshift, UV-bright galaxies (M-UV less than or similar to 21) may have low escape fractions, f(esc)(abs) less than or similar to 0.1.Conclusions. UV absorption lines trace the cold ISM gas of galaxies, which governs the physics of the LyC escape. We show that, with some assumptions, the absolute LyC escape can be statistically predicted using UV absorption lines, and the method can be applied to study galaxies across a wide redshift range, including in the epoch of cosmic reionization.
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