| 1. |
- Hegay, Sergay, et al.
(författare)
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Comparing genetic diversity and population structure of common beans grown in Kyrgyzstan using microsatellites
- 2012
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Ingår i: Scientific Journal of Crop Science. - 2322-1690. ; 1, s. 63-75
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Tidskriftsartikel (refereegranskat)abstract
- Common bean (Phaseolus vulgaris L.) is an important export crop in Kyrgyzstan. The aim of this study was to assess the extent of genetic diversity, determine the population structure, and relate to the main gene pools grown in Kyrgyzstan. Twenty-eight common bean accessions (including five Kyrgyz cultivars, and main references from the Mesoamerica and South America) were evaluated with microsatellites. Nine polymorphic microsatellites were used to estimate genetic diversity and heterozygosity. The number of alleles per microsatellite locus ranged from 2 to 4 and there were a total of 24 alleles. The observed heterozygosity of each accession over all loci ranged from 0 to 0.11 (with an average of 0.01), while the expected average heterozygosity was 0.05, which could reflect the self-pollinating breeding behavior of common beans. The analysis of molecular variance further revealed that 94.71% of the total variation was accounted by differences among accessions (Fst =0.947; p<0.001). Cluster analysis grouped accessions in two gene pools: 16 belong to the Andean and 12 to the Mesoamerican gene pool. The microsatellites separated accessions in Mesoamerican gene pool from Durango and Jalisco races, which were grouped together. We also observed that the most divergent accessions were the Kyrgyz cultivars, which may be related to the Mesoamerican races. Andean accessions were less diverse than Mesoamerican accessions in this study. This research confirms the ability of microsatellites to differentiate common bean accessions, even using a small sample size, and to be able to assign modern cultivars to their gene pools or races.
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| 2. |
- Hegay, Sergay
(författare)
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Diversity of beans grown in Kyrgyzstan and marker-aided breeding for resistance to Bean common mosaic virus and anthracnose
- 2013
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Common bean (Phaseolus vulgaris L.) is an important export crop in Kyrgyzstan since the end of the 20th century. Kyrgyzstan produces about 70,000 t of common beans per year, which provides jobs to 76% of the population in the Talas region. Information about genetic diversity of common beans helps to select appropriate genetic material to be used for breeding programs. Accessions originating from both Mesoamerican and Andean gene pools, including the main Kyrgyz common bean market types were analyzed using simple sequence repeats (SSR) and qualitative morphological traits. The similarity matrices generated from the molecular and morphological data were well correlated (r = 0.49**). The cluster analysis of both data sets grouped the accessions according to their gene pools of origin, where Mesoamerican accessions were more diverse than Andean accessions. Both SSR and qualitative morphological markers were suitable for assigning cultivars to their gene pools of origin. Furthermore, information about traits of interest for Kyrgyz farmers has been gained. We found that Bean common mosaic virus strain NL6 and anthracnose affect this crop and reduce its grain yield significantly in Kyrgyzstan. The susceptible Kyrgyz cultivars Ryabaya, Kytayanka and Lopatka were included as recurrent parents in a backcrossing breeding scheme for introducing host plant resistance to these diseases from donor cultivars. After the 4th backcross, seeds (color, shape, size) and pods (shape) were similar to the respective recurrent parent. The sequence characterized amplified region (SCAR) markers SW13, SBD5 and SCAreoli were used successfully in marker-aided backcrossing for pyramiding the I, bc-12 and Co-2 genes, which provide host plant resistance to BCMV and anthracnose, respectively. Inoculation tests with anthracnose races delta and gamma, virus strain NL3, and DNA markers confirmed the presence of resistance genes in the offspring.
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| 3. |
- Hegay, Sergay, et al.
(författare)
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Genetic diversity analysis in Phaseolus vulgaris L. using morphological traits
- 2014
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Ingår i: Genetic Resources and Crop Evolution. - : Springer Science and Business Media LLC. - 0925-9864 .- 1573-5109. ; 61, s. 555-566
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Tidskriftsartikel (refereegranskat)abstract
- Common bean (Phaseolus vulgaris) is an important export crop in Kyrgyzstan since the end of the twentieth century. Genetic diversity analysis of common bean populations is useful for breeding programs, as it helps to select genetic material to be used for further crossing. Twenty-seven common bean accessions were analyzed using 13 qualitative morphological traits. In some cases, obtained morphological data were combined with previously published results based on microsatellite markers. The similarity matrices generated from the molecular and morphological data were significantly correlated (r = 0.49, P < 0.01). Cluster analyses based on Dice's similarity coefficient were constructed based on morphological data and the combined data set of morphology and microsatellite, and both grouped the 27 accessions according to their origin: 15 belonged to the Andean and 12 to the Mesoamerican gene pool. On average, the Andean accessions were less diverse than the Mesoamerican accessions. The average diversity based on the Shannon diversity index for the 13 qualitative morphological traits was 0.05. Overall, this study revealed that qualitative morphological markers are efficient in assigning modern cultivars to their gene pools of origin.
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| 4. |
- Hegay, Sergay, et al.
(författare)
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Introducing host-plant resistance to anthracnose in Kyrgyz common bean through inoculation-based and marker-aided selection
- 2014
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Ingår i: Plant Breeding. - : Wiley. - 0179-9541 .- 1439-0523. ; 133, s. 86-91
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Tidskriftsartikel (refereegranskat)abstract
- Common bean (Phaseolus vulgaris L.) is one of the most important legume crops for human consumption. However, its grain yield can be reduced by up to 90% by the seedborne disease, anthracnose. Fungicide treatment is costly and time-consuming. The introduction of host plant resistance against this disease appears, therefore, to be crucial for enhancing the productivity of this crop in Kyrgyzstan. The use of DNA-based markers in backcrossing programmes may help speed up the breeding for resistance. In this study, we used a combination of inoculation tests and a DNA marker (SCAreoli marker) to track the transfer of host-plant resistance (Co-2 gene) from two donor cultivars, Vaillant' and Flagrano', to susceptible Kyrgyz cultivars Ryabaya', Kytayanka' and Lopatka', which are widely grown in the country. The segregating offspring were evaluated to test the reliability of the SCAreoli marker as selection aid for host-plant resistance to anthracnose. Our study showed that a co-dominant DNA marker can successfully be used in backcross breeding to distinguish segregating material in different market classes of common bean.
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