| 1. |
- Bossini-Castillo, Lara, et al.
(författare)
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Confirmation of association of the macrophage migration inhibitory factor gene with systemic sclerosis in a large European population.
- 2011
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Ingår i: Rheumatology (Oxford, England). - : Oxford University Press (OUP). - 1462-0332 .- 1462-0324. ; 50, s. 1976-1981
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Tidskriftsartikel (refereegranskat)abstract
- Objectives. The aim of this study was to confirm the implication of macrophage migration inhibitory factor (MIF) gene in SSc susceptibility or clinical phenotypes in a large European population.Methods. A total of 3800 SSc patients and 4282 healthy controls of white Caucasian ancestry from eight different European countries were included in the study. The MIF -173 single nucleotide polymorphism (SNP) was selected as genetic marker and genotyped using Taqman 5' allelic discrimination assay.Results. The MIF -173 SNP showed association with SSc [P = 0.04, odds ratio (OR) = 1.10, 95% CI 1.00, 1.19]. Analysis of the MIF -173 polymorphism according to SSc clinical phenotype revealed that the frequency of the -173*C allele was significantly higher in the dcSSc group compared with controls (P = 5.30E-03, OR = 1.21, 95% CI 1.07, 1.38). Conversely, the frequency of the MIF -173*C allele was significantly underrepresented in the lcSSc group compared with dcSSc patients, supporting previous findings [(P = 0.04, OR = 0.86, 95% CI 0.75, 0.99); meta-analysis including previous results (P = 0.005, OR = 0.83, 95% CI 0.73, 0.94)].Conclusion. Our results confirm the role of MIF -173 promoter polymorphism in SSc, and provide evidence of a strong association with the dcSSc subgroup of patients. Hence, the MIF -173 variant is confirmed as a promising clinical phenotype genetic marker.
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| 2. |
- López-Isac, Elena, et al.
(författare)
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Brief Report : IRF4 Newly Identified as a Common Susceptibility Locus for Systemic Sclerosis and Rheumatoid Arthritis in a Cross-Disease Meta-Analysis of Genome-Wide Association Studies
- 2016
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Ingår i: Arthritis & Rheumatology. - : Wiley. - 2326-5191 .- 2326-5205. ; 68:9, s. 2338-2344
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Tidskriftsartikel (refereegranskat)abstract
- Objective: Systemic sclerosis (SSc) and rheumatoid arthritis (RA) are autoimmune diseases that have similar clinical and immunologic characteristics. To date, several shared SSc–RA genetic loci have been identified independently. The aim of the current study was to systematically search for new common SSc–RA loci through an interdisease meta–genome-wide association (meta-GWAS) strategy. Methods: The study was designed as a meta-analysis combining GWAS data sets of patients with SSc and patients with RA, using a strategy that allowed identification of loci with both same-direction and opposite-direction allelic effects. The top single-nucleotide polymorphisms were followed up in independent SSc and RA case–control cohorts. This allowed an increase in the sample size to a total of 8,830 patients with SSc, 16,870 patients with RA, and 43,393 healthy controls. Results: This cross-disease meta-analysis of the GWAS data sets identified several loci with nominal association signals (P < 5 × 10−6) that also showed evidence of association in the disease-specific GWAS scans. These loci included several genomic regions not previously reported as shared loci, as well as several risk factors that were previously found to be associated with both diseases. Follow-up analyses of the putatively new SSc–RA loci identified IRF4 as a shared risk factor for these 2 diseases (Pcombined = 3.29 × 10−12). Analysis of the biologic relevance of the known SSc–RA shared loci identified the type I interferon and interleukin-12 signaling pathways as the main common etiologic factors. Conclusion: This study identified a novel shared locus, IRF4, for the risk of SSc and RA, and highlighted the usefulness of a cross-disease GWAS meta-analysis strategy in the identification of common risk loci.
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| 3. |
- Calderon-González, Karla Gisel, et al.
(författare)
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Cryptic in vitro ubiquitin ligase activity of HDMX towards p53 is probably regulated by an induced fit mechanism
- 2022
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Ingår i: Bioscience Reports. - : Portland Press. - 0144-8463 .- 1573-4935. ; 42:7
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Tidskriftsartikel (refereegranskat)abstract
- HDMX and its homologue HDM2 are two essential proteins for the cell; after genotoxic stress, both are phosphorylated near to their RING domain, specifically at serine 403 and 395, respectively. Once phosphorylated, both can bind the p53 mRNA and enhance its translation; however, both recognize p53 protein and provoke its degradation under normal conditions. HDM2 has been well-recognized as an E3 ubiquitin ligase, whereas it has been reported that even with the high similarity between the RING domains of the two homologs, HDMX does not have the E3 ligase activity. Despite this, HDMX is needed for the proper p53 poly-ubiquitination. Phosphorylation at serine 395 changes the conformation of HDM2, helping to explain the switch in its activity, but no information on HDMX has been published. Here, we study the conformation of HDMX and its phospho-mimetic mutant S403D, investigate its E3 ligase activity and dissect its binding with p53. We show that phospho-mutation does not change the conformation of the protein, but HDMX is indeed an E3 ubiquitin ligase in vitro; however, in vivo, no activity was found. We speculated that HDMX is regulated by induced fit, being able to switch activity accordingly to the specific partner as p53 protein, p53 mRNA or HDM2. Our results aim to contribute to the elucidation of the contribution of the HDMX to p53 regulation.
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| 4. |
- Fasth Berglund, Åsa, 1978, et al.
(författare)
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Cognitive automation strategie: Improving use-efficiency of carrier and content of information
- 2014
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Ingår i: Procedia CIRP. - : Elsevier BV. - 2212-8271. ; 17, s. 67-70
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Konferensbidrag (refereegranskat)abstract
- The production paradigm of mass customization puts high demand on the production system and the people who works there. This is especially true for final assembly where the number of variants is the richest. To handle this variety, the operator needs the correct information accessible at the right time; the operator wants to know how and when to assemble what part. What is correct information includes the amount of information (content of information), how it is presented (carrier of information) and who is the receiver (specific operator). The strategy for Cognitive Automation will be more and more important for companies within this paradigm. This paper aims to show the use-Efficiency of information, both carrier and content, in two industrial case studies. Furthermore how the cognitive automation strategy within a company is connected to the maturity of technology. ©2014 Elsevier B.V. This is an open access article under the CC BY-NC-ND license.
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| 5. |
- Ferrando, Carlos, et al.
(författare)
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Effects of oxygen on post-surgical infections during an individualised perioperative open-lung ventilatory strategy : a randomised controlled trial
- 2020
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Ingår i: British Journal of Anaesthesia. - : ELSEVIER SCI LTD. - 0007-0912 .- 1471-6771. ; 124:1, s. 110-120
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Tidskriftsartikel (refereegranskat)abstract
- Background: We aimed to examine whether using a high fraction of inspired oxygen (FIO2) in the context of an individualised intra- and postoperative open-lung ventilation approach could decrease surgical site infection (SSI) in patients scheduled for abdominal surgery. Methods: We performed a multicentre, randomised controlled clinical trial in a network of 21 university hospitals from June 6, 2017 to July 19, 2018. Patients undergoing abdominal surgery were randomly assigned to receive a high (0.80) or conventional (0.3) FIO2 during the intraoperative period and during the first 3 postoperative hours. All patients were mechanically ventilated with an open-lung strategy, which included recruitment manoeuvres and individualised positive end-expiratory pressure for the best respiratory-system compliance, and individualised continuous postoperative airway pressure for adequate peripheral oxyhaemoglobin saturation. The primary outcome was the prevalence of SSI within the first 7 postoperative days. The secondary outcomes were composites of systemic complications, length of intensive care and hospital stay, and 6-month mortality. Results: We enrolled 740 subjects: 371 in the high FIO2 group and 369 in the low FIO2 group. Data from 717 subjects were available for final analysis. The rate of SSI during the first postoperative week did not differ between high (8.9%) and low (9.4%) FIO2 groups (relative risk [RR]: 0.94; 95% confidence interval [CI]: 0.59-1.50; P=0.90]). Secondary outcomes, such as atelectasis (7.7% vs 9.8%; RR: 0.77; 95% CI: 0.48-1.25; P=0.38) and myocardial ischaemia (0.6% [n=2] vs 0% [n=0]; P=0.47) did not differ between groups. Conclusions: An oxygenation strategy using high FIO2 compared with conventional FIO2 did not reduce postoperative SSIs in abdominal surgery. No differences in secondary outcomes or adverse events were found.
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| 6. |
- Gallego-Parra, Samuel, et al.
(författare)
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Pressure-induced order-disorder transitions in beta-In2S3 : an experimental and theoretical study of structural and vibrational properties
- 2021
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Ingår i: Physical Chemistry, Chemical Physics - PCCP. - : Royal Society of Chemistry. - 1463-9076 .- 1463-9084. ; 23:41, s. 23625-23642
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Tidskriftsartikel (refereegranskat)abstract
- This joint experimental and theoretical study of the structural and vibrational properties of beta-In2S3 upon compression shows that this tetragonal defect spinel undergoes two reversible pressure-induced order-disorder transitions up to 20 GPa. We propose that the first high-pressure phase above 5.0 GPa has the cubic defect spinel structure of alpha-In2S3 and the second high-pressure phase (phi-In2S3) above 10.5 GPa has a defect alpha-NaFeO2-type (R3m) structure. This phase, related to the NaCl structure, has not been previously observed in spinels under compression and is related to both the tetradymite structure of topological insulators and to the defect LiTiO2 phase observed at high pressure in other thiospinels. Structural characterization of the three phases shows that alpha-In2S3 is softer than beta-In2S3 while phi-In2S3 is harder than beta-In2S3. Vibrational characterization of the three phases is also provided, and their Raman-active modes are tentatively assigned. Our work shows that the metastable alpha phase of In2S3 can be accessed not only by high temperature or varying composition, but also by high pressure. On top of that, the pressure-induced beta-alpha-phi sequence of phase transitions evidences that beta-In2S3, a BIII2XV3 compound with an intriguing structure typical of A(II)BIII(2)XVI(4) compounds (intermediate between thiospinels and ordered-vacancy compounds) undergoes: (i) a first phase transition at ambient pressure to a disordered spinel-type structure (alpha-In2S3), isostructural with those found at high pressure and high temperature in other BIII2XV3 compounds; and (ii) a second phase transition to the defect alpha-NaFeO2-type structure (phi-In2S3), a distorted NaCl-type structure that is related to the defect NaCl phase found at high pressure in A(II)BIII(2)XVI(4) ordered-vacancy compounds and to the defect LiTiO2-type phase found at high pressure in A(II)BIII(2)XVI(4) thiospinels. This result shows that In2S3 (with its intrinsic vacancies) has a similar pressure behaviour to thiospinels and ordered-vacancy compounds of the A(II)BIII(2)XVI(4) family, making beta-In2S3 the union link between such families of compounds and showing that group-13 thiospinels have more in common with ordered-vacancy compounds than with oxospinels and thiospinels with transition metals.
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| 7. |
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| 8. |
- Garrido Hernandez, Vanesa, 1974, et al.
(författare)
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ICT-TOOLS SUPPORT TOYOTA WAY PRINCIPLE #6
- 2014
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Ingår i: The sixth Swedish Production Symposium. - 9789198097412
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Konferensbidrag (refereegranskat)abstract
- This paper pursues to discuss how the implementation of Information and Communication Technologies (ICT)-tools could support standardized work (principle #6). This will be done through a theoretical discussion, and furthermore a case study will show a practical example i.e, a web application as an ICT-tool solution to support standardized tasks. The web application is intended to; help the standardization of assembly instructions, help learn novice and experienced operators, and increase the knowledge sharing. The case study has no documented instructions, as a consequence, there is a high risk that all this knowledge can be lost in the future.
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| 9. |
- Garrido Hernandez, Vanesa, 1974
(författare)
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Information and Communication Technologies and Lean Production: striving for a synergetic integration
- 2016
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The role and integration of Information and Communication Technology (ICT) in lean production (LP) has been studied for decades. Research has shown that such technology and lean production complement each other and are interdependent in some cases. Continuing with this research, the purposes of this research work are to elaborate on the role and integration of ICT in lean production, and to ensure the success of the ICT tools that will support lean production. The theoretical findings revealed that ICT has two main roles in lean production systems: to help implement lean production, and to enhance lean principles. One of the lean principles is standardized work. The integration of ICT tools such as Web application prototypes and Quick Response (QR) codes was studied in different scenarios that included a standardized process. The empirical findings showed that Web applications provide ubiquity, which enables access to information everywhere and reaching more workers. With QR codes, it is possible to provide the right information at the right time and at the right place. Moreover, a theoretical framework is proposed, the ICT-LP integration matrix. This framework aims to help integrate ICT in lean production implementations by identifying different scenarios and their challenges and constraints. The development of this framework is based on a theoretical elaboration and empirical studies. The theoretical elaboration was carried out by the comparison of the integration matrix and a conceptual model found in the literature. Partial empirical results found in this research identified challenges for ICT, such as avoiding affecting the good habits between workers like teamwork and providing the adequate infrastructure. However, the success of ICT in terms of its design, development and implementation is complex and elusive. In fact, the theoretical findings have shown that ICT has a positive impact in LP implementations, but this does not ensure a successful integration at all. Knowing this, a consolidated framework was developed to assess success. Partial empirical testing of the framework let us evaluate some ICT prototype tools that support standardized work.
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| 10. |
- Garrido Hernandez, Vanesa, 1974, et al.
(författare)
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Web application + QR codes: an ICT tool to ease access of information and support standardized work
- 2015
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Ingår i: The International Conference on Flexible Automation and Intelligent Manufacturing (FAIM). - 9781910864005
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Konferensbidrag (refereegranskat)abstract
- Information and Communication Technology (ICT) may offer advantages to organizations, if it is well implemented and aligned to the organization’s goal(s), production system and/or business model. On the other hand, lean manufacturing may not succeed if the core concepts are misinterpreted. One of them, standardization, is the pillar for continuous improvement. Synergy is expected by using ICT tools to support lean in the standardization process. However, such combination of powerful concepts could create challenges for both. The aim of the presented work is to evaluate and validate how the use of ICT tools, - a Web application prototype and QR codes, could support the standardization process at a stable phase, and ease the access of information through QR codes. Different challenges were found while testing the tool, which will be further discussed. In general, the tool was perceived as useful and easy to use. The cycle time was measured to validate the effectiveness of the tool compared to the use of printed information. The main benefit was it eases the access to information, when the organization and control of document rules make hard to find the correct information at the right time.
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