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- Hoa, NK, et al.
(författare)
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The possible mechanisms by which phanoside stimulates insulin secretion from rat islets
- 2007
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Ingår i: The Journal of endocrinology. - : Bioscientifica. - 0022-0795 .- 1479-6805. ; 192:2, s. 389-394
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Tidskriftsartikel (refereegranskat)abstract
- We recently showed that phanoside, a gypenoside isolated from the plant Gynostemma pentaphyllum, stimulates insulin secretion from rat pancreatic islets. To study the mechanisms by which phanoside stimulates insulin secretion. Isolated pancreatic islets of normal Wistar (W) rats and spontaneously diabetic Goto-Kakizaki (GK) rats were batch incubated or perifused. At both 3.3 and 16.7 mM glucose, phanoside stimulated insulin secretion several fold in both W and diabetic GK rat islets. In perifusion of W islets, phanoside (75 and 150 μM) dose dependently increased insulin secretion that returned to basal levels when phanoside was omitted. When W rat islets were incubated at 3.3 mM glucose with 150 μM phanoside and 0.25 mM diazoxide to keep K-ATP channels open, insulin secretion was similar to that in islets incubated in 150 μM phanoside alone. At 16.7 mM glucose, phanoside-stimulated insulin secretion was reduced in the presence of 0.25 mM diazoxide (P<0.01). In W islets depolarized by 50 mM KCl and with diazoxide, phanoside stimulated insulin release twofold at 3.3 mM glucose but did not further increase the release at 16.7 mM glucose. When using nimodipine to block L-type Ca2+ channels in B-cells, phanoside-induced insulin secretion was unaffected at 3.3 mM glucose but decreased at 16.7 mM glucose (P<0.01). Pretreatment of islets with pertussis toxin to inhibit exocytotic Ge-protein did not affect insulin response to 150 μM phanoside. Phanoside stimulated insulin secretion from Wand GK rat islets. This effect seems to be exerted distal to K-ATP channels and L-type Ca2+ channels, which is on the exocytotic machinery of the B-cells.
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- Huyen, VTT, et al.
(författare)
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Antidiabetic Effects of Add-On Gynostemma pentaphyllum Extract Therapy with Sulfonylureas in Type 2 Diabetic Patients
- 2012
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Ingår i: Evidence-based complementary and alternative medicine : eCAM. - : Hindawi Limited. - 1741-4288 .- 1741-427X. ; 2012, s. 452313-
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Tidskriftsartikel (refereegranskat)abstract
- Aims.To investigate the antidiabetic effect of the traditional Vietnamese herbGynostemma pentaphyllum(GP) together with sulfonylurea (SU) in 25 drug-naïve type 2 diabetic patients.Methods.After 4-week treatment with gliclazide (SU), 30 mg daily, all patients were randomly assigned into 2 groups to add on GP extract or placebo extract, 6 g daily, during eight weeks.Results.After 4-week SU treatment, fasting plasma glucose (FPG) and HbA1Cdecreased significantly (P<0.001). FPG was further reduced after add-on therapy with 2.9 ± 1.7 and 0.9 ± 0.6 mmol/L in the GP and placebo groups, respectively (P<0.001). Therapy with GP extract also reduced 30- and 120-minute oral glucose tolerance test postload values. HbA1Clevels decreased approximately 2% units in the GP group compared to 0.7% unit in the placebo group (P<0.001).Conclusion.GP extract in addition to SU offers an alternative to addition of other oral medication to treat type 2 diabetic patients.
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- Huyen, VT, et al.
(författare)
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Gynostemma pentaphyllum Tea Improves Insulin Sensitivity in Type 2 Diabetic Patients
- 2013
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Ingår i: Journal of nutrition and metabolism. - : Hindawi Limited. - 2090-0724 .- 2090-0732. ; 2013, s. 765383-
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Tidskriftsartikel (refereegranskat)abstract
- Aims. To evaluate the effect of the traditional Vietnamese herbGynostemma pentaphyllumtea on insulin sensitivity in drug-naïve type 2 diabetic patients.Methods. Patients received GP or placebo tea 6 g daily for four weeks and vice versa with a 2-week wash-out period. At the end of each period, a somatostatin-insulin-glucose infusion test (SIGIT) was performed to evaluate the insulin sensitivity. Fasting plasma glucose (FPG),HbA1C, and oral glucose tolerance tests and insulin levels were measured before, during, and after the treatment.Results. FPG and steady-state plasma glucose (SIGIT mean) were lower after GP treatment compared to placebo treatment (P<0.001). The levels of FPG in the control group were slightly reduced to0.2±1.5versus1.9±1.0 mmol/L in GP group (P<0.001), and the effect on FPG was reversed after exchanging treatments. The glycometabolic improvements were achieved without any major change of circulating insulin levels. There were no changes in lipids, body measurements, blood pressure, and no reported hypoglycemias or acute adverse effects regarding kidney and liver parameters.Conclusion. The results of this study suggested that the GP tea exerted antidiabetic effect by improving insulin sensitivity.
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