| 1. |
- Trinks, Cecilia, et al.
(författare)
-
The pan-ErbB tyrosine kinase inhibitor canertinib induces caspase-mediated cell death in human T-cell leukemia (Jurkat) cells
- 2011
-
Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier Science B.V., Amsterdam. - 0006-291X .- 1090-2104. ; 410:3, s. 422-427
-
Tidskriftsartikel (refereegranskat)abstract
- Canertinib is a novel ErbB-receptor inhibitor currently in clinical development for the treatment of solid tumors overexpressing ErbB-receptors. We have recently demonstrated that canertinib displays anti-proliferative and pro-apoptotic effects in human myeloid leukemia cells devoid of ErbB-receptors. The mechanism mediating these effects are however unknown. In this study, we show that canertinib is able to act as a multi-kinase inhibitor by inhibition of several intracellular kinases involved in T-cell signaling such as Akt, Erk1/2 and Zap-70, and reduced Lck protein expression in the human T-cell leukemia cell line Jurkat. Treatment with canertinib at a concentration of 2 mu M caused accumulation of Jurkat cells in the G(1) cell cycle phase and increased doses induced apoptosis in a time-dependent manner. Apoptotic signs of treated cells were detected by Annexin V staining and cleavage of PARP, caspase-3, -8, -9, -10 and Bid. A subset of the pro-apoptotic signals mediated by canertinib could be significantly reduced by specific caspase inhibitors. Taken together, these results demonstrate the dual ability of canertinib to downregulate important signaling pathways and to activate caspase-mediated intrinsic apoptosis pathway in human T-cell leukemia cells.
|
|
| 2. |
- Holmlund, Anna, 1987
(författare)
-
Different ways of experiencing linear equations with a multiplicative structure
- 2022
-
Ingår i: Bringing Nordic mathematics education into the future. - Oslo : Svensk förening för MatematikDidaktiskForskning. - 1651-3274. - 9789198402469 ; 17, s. 89-96
-
Konferensbidrag (refereegranskat)abstract
- Learning to solve linear equations of the form a=b∙x with decimal fractions as coefficients is relevant to all secondary school students and especially for students in vocational education, as it can be significant for their future profession (e.g., Ohm’s law). This study reports on students’ different ways of experiencing equations depending on whether whole numbers or decimal fractions are used. In semi-structured interviews, students displayed an uncertainty towards equations containing decimal fractions and did not address them with the same powerful method as they used for equations with whole numbers, even though they had access to a calculator. The findings emphasize the importance of using different kinds of numbers when teaching equation solving.
|
|
| 3. |
- Holmlund, Anna, 1987
(författare)
-
How numbers influence students when solving linear equations
- 2024
-
Ingår i: MATHEMATICAL THINKING AND LEARNING. - 1098-6065 .- 1532-7833.
-
Tidskriftsartikel (refereegranskat)abstract
- Most students need to learn to solve equations as a part of their education, but far from all succeed. In this study we investigate the potential impact of variation in the choice of numbers as coefficients when students in upper secondary school solve linear equations. A test with linear equations was distributed to 111 16-year-old vocational students and phenomenographic interviews were performed with 23 of them. The findings show that exchanging coefficients (e.g., changing a and b in $a = b\cdot x$a=b & sdot;x) from natural numbers to other numbers significantly changes how equations with the same structure are handled, despite access to a calculator. A framework of five conceptions is described explaining how numbers are experienced in the foreground or background of attention, partly depending on what meaning the equation is given. The results indicate that number types need to be considered, not only in teaching arithmetic, but also in algebra.
|
|
| 4. |
- Holmlund, Anna, 1987
(författare)
-
The influence of numbers when students solve equations
- 2023
-
Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Is it possible that some students’ primary difficulty with equation-solving is neither handling the literal symbols nor the equality, but the numbers used as coefficients? It is well known that many students find algebra a difficult topic, and there is much research on how students experience this strand of mathematics, with indications of how it can be taught. Still, a perspective not often fronted in this research – that has been suggested as an area potentially important – is how numbers, other than natural numbers, in algebra, are perceived by students. Such kinds of numbers (negative numbers and decimal fractions) have been used in this thesis to explore how the numbers influence students’ equation-solving. Two studies with a phenomenographic approach have explored how students (n1=5, n2=23) perceive linear equations of similar structure but with different kinds of numbers as coefficients, e.g., 819=39∙? and 0.12=0.4∙?. In the second study, a test was also used to investigate the magnitude of the influence of a change of coefficients for 110 students while solving equations with a calculator. The findings show that equations with decimal fractions and negative numbers are less likely to be solved by these students, and decimal fractions as coefficients can even make a student unable to recognize a kind of equation they just solved with natural numbers. The interviews display that, depending on the number in a linear equation, some students focus on different aspects of the equation, and that the numbers influence what meaning the students see in the equation and how they can justify their solution. Following the phenomenographic approach, differences in the way that students experience the equations were specified, and critical aspects were formulated. This implies a wider use of different kinds of numbers in teaching algebra, as different kinds of numbers hold different challenges, thereby also varying learning potential, for students.
|
|
| 5. |
- Holmlund, Anna, 1987
(författare)
-
The work done when solving linear equations: Altering experiences due to change of numbers
- 2024
-
Ingår i: Proceedings of the Thirteenth Congress of the European Society for Research in Mathematics Education. - Budapest, Hungary : Alfréd Rényi Institute of Mathematics; Eötvös Loránd University of Budapest. - 9789637031045
-
Konferensbidrag (refereegranskat)abstract
- This paper investigates how one student solves linear equations with a focus on how different numbers (e.g., natural numbers, numbers with decimals, and negatives) in the equations influence reasoning. The choice of coefficients in equations is not usually the focus in research nor in teaching. However, as students are used to taking numbers into consideration, the influence of coefficients on equation solving is an important topic to address. In a previous study, a model for how students experience equations was suggested. Here, the model is used to analyse one interview with a student in upper-secondary school, solving several similar equations but with numbers from varying number domains. The results show the decisive role that numbers play in how this student perceives the equations. Using a wide range of numbers also helped to challenge the student to explore the properties of the equations’ structure. Possible applications in the classroom are discussed.
|
|
| 6. |
- Holmlund, Camilla, 1969-, et al.
(författare)
-
Characterization and tissue-specific expression of human LRIG2
- 2004
-
Ingår i: Gene. - : Elsevier BV. - 0378-1119 .- 1879-0038. ; 332, s. 35-43
-
Tidskriftsartikel (refereegranskat)abstract
- We have recently identified and cloned the human LRIG1 gene (formerly LIG1). LRIG1 is a predicted integral membrane protein with a domain organization reminiscent of the Drosophila epidermal growth factor (EGF)-receptor antagonist Kekkon-1. We have searched for additional members of the human LRIG family and identified LRIG2 (KIAA0806). The LRIG2 gene was localized to chromosome 1p13 and had an open reading frame of 1065 amino acids. The LRIG2 protein was predicted to have the same domain organization as LRIG1 with a signal peptide, an extracellular part containing15 leucine-rich repeats and three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. The LRIG2 amino acid sequence was 47% identical to human LRIG1 and mouse Lrig1 (also known as Lig-1). Northern blotting and RT-PCR revealed LRIG2 transcripts in all tissues analyzed. Quantitative real-time RT-PCR showed the most prominent RNA expression in skin, uterus, ovary, kidney, brain, small intestine, adrenal gland, and stomach. Immunoblotting of COS-7 cell lysates demonstrated that heterologously expressed human LRIG2 had an apparent molecular weight of 132 kDa under reducing gel-running conditions. N-glycosidase F treatment resulted in a reduction of the apparent molecular weight to 107 kDa, showing that LRIG2 was a glycoprotein carrying N-linked oligosaccharides. Cell surface biotinylation experiments and confocal fluorescence laser microscopy demonstrated expression of LRIG2 both at the cell surface and in the cytoplasm. LRIG2 was detected in tissue lysates from stomach, prostate, lung, and fetal brain by immunoblotting. In conclusion, LRIG2 was found to be a glycoprotein which was encoded by a gene on human chromosome 1p13 and its mRNA was present in all tissues analyzed.
|
|
| 7. |
- Holmlund, Lisa, et al.
(författare)
-
Are psychosocial work factors and work-home interference associated with time to first full return-to-work after sick leave due to common mental disorders?
- 2023
-
Ingår i: International Archives of Occupational and Environmental Health. - : Springer. - 0340-0131 .- 1432-1246. ; 96, s. 747-755
-
Tidskriftsartikel (refereegranskat)abstract
- ObjectiveTo (1) examine the time to first full return-to-work (RTW), and (2) investigate whether psychosocial work factors and work-home interference are associated with time to first full RTW after sick leave due to common mental disorders (CMDs).MethodsThe cohort study comprised 162 employees on sick leave due to CMDs participating in a two-armed cluster-randomised controlled trial in Sweden. Baseline data consisted of a web-based questionnaire and follow-up data of repeated text messages every fourth week for 12 months. The time to first full RTW was estimated using the Kaplan–Meier Estimator. Parametric Weibull survival models with interval-censored outcomes were used to determine associations between psychosocial work factors and work-home interference with time to first full RTW. In a post hoc analysis, time-interval differences in associations for 0– ≤ 6- versus > 6–12 months were tested.ResultsDuring the 12-month follow-up, n = 131 (80.9%) reported a first full RTW. The median time to this RTW was 16 weeks (95% CI 12; 20). High psychological job demands, high emotional job demands, high work-to-home interference (WHI), and low social job support were independently associated with a longer time to first full RTW. Time-interval differences were found for job control and emotional job demands.ConclusionsPsychosocial work demands and WHI are associated with a longer time to RTW after sick leave due to CMDs. Work organisations and rehabilitation practices should include accommodations for high psychological and emotional job demands during RTW, as well as pay attention to the risk of spill-over of high job demands into employees’ private lives.
|
|
| 8. |
|
|
| 9. |
- Holmlund, Sophia, et al.
(författare)
-
Set aside-A qualitative study of partners' experiences of pregnancy, labour, and postnatal care in Sweden during the COVID-19 pandemic.
- 2024
-
Ingår i: PloS one. - 1932-6203. ; 19:9
-
Tidskriftsartikel (refereegranskat)abstract
- Due to changes in Swedish maternity care during the COVID-19 pandemic, partners were often excluded from antenatal and postnatal care.To explore partners' experiences of pregnancy, labour, and postnatal care in relation to the COVID-19 pandemic restrictions.A descriptive qualitative interview study with 15 partners of women who gave birth from March 2020 to March 2022. Data was collected from April to November 2022, and analysed using inductive thematic analysis.Two themes and six subthemes were identified. The first theme, Feelings of loss and exclusion, emphasises the expectation and desire to share the journey of becoming a parent together with the pregnant partner. When excluded from maternity care, a feeling of missing out was described which could create a sense of distance from the unborn child. The second theme, Dealing with powerlessness, relates to the fear of infection and not being able to participate during the birth, and life being adapted to restrictions. Mixed feelings regarding the restrictions were described since the reasons behind were not always perceived as clear and logical.Sweden prides itself on gender equality, where partners normally are a natural part of maternity care. This likely contributed to strong feelings of exclusion when partners were prevented from participating in maternity care during the COVID-19 pandemic.Partners of women giving birth during the COVID-19 pandemic were substantially affected by the restrictions within maternity care. Partners wish to be involved in pregnancy and birth and want to receive clear information as part of their preparation for parenthood. Society-including maternity care-must decide how to address these needs.
|
|
| 10. |
- Jornvall, Hans, et al.
(författare)
-
Oligomerization and insulin interactions of proinsulin C-peptide : Threefold relationships to properties of insulin
- 2010
-
Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 391:3, s. 1561-1566
-
Tidskriftsartikel (refereegranskat)abstract
- Three principally different sites of action have been reported for proinsulin C-peptide, at surface-mediated, intracellular, and extracellular locations. Following up on the latter, we now find that (i) mass spectrometric analyses reveal the presence of the C-peptide monomer in apparent equilibrium with a low-yield set of oligomers in weakly acidic or basic aqueous solutions, even at low peptide concentrations (sub-mu M). It further shows not only C-peptide to interact with insulin oligomers (known before), but also the other way around. (ii) Polyacrylamide gel electrophoresis of C-peptide shows detectable oligomers upon Western blotting. Formation of thioflavin T positive material was also detected. (iii) Cleavage patterns of analogues are compatible with C-peptide as a substrate of insulin degrading enzyme. Combined, the results demonstrate three links with insulin properties, in a manner reminiscent of amyloidogenic peptides and their chaperons in other systems. If so, peripheral C-peptide/insulin interactions, absolute amounts of both peptides and their ratios may be relevant to consider in diabetic and associated diseases.
|
|
