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Sökning: WFRF:(Honigmann A)

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  • Woodruff, J. B., et al. (författare)
  • The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin
  • 2017
  • Ingår i: Cell. - : Elsevier BV. - 0092-8674. ; 169:6, s. 1066-1077
  • Tidskriftsartikel (refereegranskat)abstract
    • Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays. They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic assembly of protein called the pericentriolar material (PCM). To study how PCM forms a spherical compartment that nucleates microtubules, we reconstituted PCM-dependent microtubule nucleation in vitro using recombinant C. elegans proteins. We found that macromolecular crowding drives assembly of the key PCM scaffold protein SPD-5 into spherical condensates that morphologically and dynamically resemble in vivo PCM. These SPD-5 condensates recruited the microtubule polymerase ZYG-9 (XMAP215 homolog) and the microtubule-stabilizing protein TPXL-1 (TPX2 homolog). Together, these three proteins concentrated tubulin similar to 4-fold over background, which was sufficient to reconstitute nucleation of microtubule asters in vitro. Our results suggest that in vivo PCM is a selective phase that organizes microtubule arrays through localized concentration of tubulin by microtubule effector proteins.
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  • Birkeland, A., et al. (författare)
  • The ultrasound visualization pipeline
  • 2014
  • Ingår i: Mathematics and Visualization. - London : springer berlin. - 1612-3786. ; 37, s. 283-303
  • Tidskriftsartikel (refereegranskat)abstract
    • Radiology is one of themain tools inmodernmedicine.Anumerous set of deceases, ailments and treatments utilize accurate images of the patient. Ultrasound is one of the most frequently used imaging modality in medicine. The high spatial resolution, its interactive nature and non-invasivenessmakes it the first choice inmany examinations. Image interpretation is one of ultrasound’s main challenges. Much training is required to obtain a confident skill level in ultrasound-based diagnostics. State-of-the-art graphics techniques is needed to providemeaningful visualizations of ultrasound in real-time. In this paper we present the process-pipeline for ultrasound visualization, including an overview of the tasks performed in the specific steps. To provide an insight into the trends of ultrasound visualization research, we have selected a set of significant publications and divided them into a technique-based taxonomy covering the topics pre-processing, segmentation, registration, rendering and augmented reality. For the different technique types we discuss the difference between ultrasound-based techniques and techniques for other modalities.
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  • Resultat 1-8 av 8

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