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Sökning: WFRF:(Hsieh Chang Yang)

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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  • Lin, Chang, et al. (författare)
  • Green aerator using periodically oscillatory flow generated inside a vertical dropping pool
  • 2012
  • Konferensbidrag (refereegranskat)abstract
    • The flow conditions for generating periodically oscillatory flows and thus entraining a large amount of air bubbles into the water mass in a vertical dropping pool, which can be used potentially as a green aerator, were investigated experimentally. The approaching flows passing through upstream reach of a vertical dropping pool were studied under sub-critical condition. A wave gauge was used to measure the free surface fluctuations in the pool, and flow visualization technique was employed to reveal the flow structure of the dropping flows qualitatively. Under certain conditions, the falling flow over a vertical dropping pool forms a switching jet that oscillates up and down periodically and impinges on the bottom and the downstream corner of the pool alternately. The switching jet switches between an impinging jet (or napped flow) and a sliding jet (or skimming flow), causing it to oscillate periodically with a unique period and to entrain a large number of air bubbles into the switching jet, thus enhancing the dissolved oxygen quantity and turbulent mixing. The primary frequency of the periodic oscillation was determined by applying spectral analysis to the time history of wave-gauge measurements for the free surface elevation of the dropping flows. Variables influencing the fundamental oscillation frequency were carefully checked, and an empirical relation between a weighted Strouhal number and a grouped non-dimensional parameter was proposed to predict the primary frequency of the periodically oscillatory flow.
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  • Resultat 1-10 av 23

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