| 1. |
- Chang, Keke, et al.
(författare)
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Considerations on Circuit Design and Data Acquisition of a Portable Surface Plasmon Resonance Biosensing System
- 2015
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Ingår i: Sensors. - : MDPI AG. - 1424-8220. ; 15:8, s. 20511-20523
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to develop a circuit for an inexpensive portable biosensing system based on surface plasmon resonance spectroscopy. This portable biosensing system designed for field use is characterized by a special structure which consists of a microfluidic cell incorporating a right angle prism functionalized with a biomolecular identification membrane, a laser line generator and a data acquisition circuit board. The data structure, data memory capacity and a line charge-coupled device (CCD) array with a driving circuit for collecting the photoelectric signals are intensively focused on and the high performance analog-to-digital (A/D) converter is comprehensively evaluated. The interface circuit and the photoelectric signal amplifier circuit are first studied to obtain the weak signals from the line CCD array in this experiment. Quantitative measurements for validating the sensitivity of the biosensing system were implemented using ethanol solutions of various concentrations indicated by volume fractions of 5%, 8%, 15%, 20%, 25%, and 30%, respectively, without a biomembrane immobilized on the surface of the SPR sensor. The experiments demonstrated that it is possible to detect a change in the refractive index of an ethanol solution with a sensitivity of 4.99838 × 10(5) ΔRU/RI in terms of the changes in delta response unit with refractive index using this SPR biosensing system, whereby the theoretical limit of detection of 3.3537 × 10(-5) refractive index unit (RIU) and a high linearity at the correlation coefficient of 0.98065. The results obtained from a series of tests confirmed the practicality of this cost-effective portable SPR biosensing system.
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| 2. |
- Hu, Jiandong, et al.
(författare)
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Development of a Surface Plasmon Resonance Biosensing Approach for the Rapid Detection of Porcine Circovirus Type2 in Sample Solutions
- 2014
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 9:10, s. e111292-
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Tidskriftsartikel (refereegranskat)abstract
- A sensitive and label-free analytical approach for the detection of porcine circovirus type 2 (PCV2) instead of PCV2 antibody in serum sample was systematically investigated in this research based on surface plasmon resonance (SPR) with an establishment of special molecular identification membrane. The experimental device for constructing the biosensing analyzer is composed of an integrated biosensor, a home-made microfluidic module, and an electrical control circuit incorporated with a photoelectric converter. In order to detect the PCV2 using the surface plasmon resonance immunoassay, the mercaptopropionic acid has been used to bind the Au film in advance through the known form of the strong S-Au covalent bonds formed by the chemical radical of the mercaptopropionic acid and the Au film. PCV2 antibodies were bonded with the mercaptopropionic acid by covalent -CO-NH- amide bonding. For the purpose of evaluating the performance of this approach, the known concentrations of PCV2 Cap protein of 10 mu g/mL, 7.5 mu g/mL, 5 mu g/mL, 2.5 mu g/mL, 1 mu g/mL, and 0.5 mu g/mL were prepared by diluting with PBS successively and then the delta response units (Delta RUs) were measured individually. Using the data collected from the linear CCD array, the Delta RUs gave a linear response over a wide concentration range of standard known concentrations of PCV2 Cap protein with the R-Squared value of 0.99625. The theoretical limit of detection was calculated to be 0.04 mu g/mL for the surface plasmon resonance biosensing approach. Correspondingly, the recovery rate ranged from 81.0% to 89.3% was obtained. In contrast to the PCV2 detection kits, this surface plasmon resonance biosensing system was validated through linearity, precision and recovery, which demonstrated that the surface plasmon resonance immunoassay is reliable and robust. It was concluded that the detection method which is associated with biomembrane properties is expected to contribute much to determine the PCV2 in sample solutions instead of PCV2 antibody in serum samples quantitatively.
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| 3. |
- Luo, Jing, et al.
(författare)
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Strong and Multifunctional Lignin/Liquid Metal Hydrogel Composite as Flexible Strain Sensors
- 2024
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Ingår i: ACS Sustainable Chemistry & Engineering. - 2168-0485. ; 12:18, s. 7105-7114
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Tidskriftsartikel (refereegranskat)abstract
- Conductive hydrogel is a promising material for flexible sensors due to its good electrical conductivity, adhesion, and high sensitivity. However, the challenge of integrating conductive fillers like liquid metals lies in their poor interface compatibility, which adversely affects their mechanical strength and lifespan. Addressing the challenge of achieving both mechanical strength and conductivity, this study harnessed 2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO)-oxidized lignin to form a stable interface between the liquid metal (LM) and the hydrogel system. Furthermore, this innovation (lignin-LM) facilitates the free radical polymerization of acrylic acid (PAA) at room temperature, resulting in conductive hydrogels. These hydrogels demonstrated excellent self-healing, adhesive, tensile, and antibacterial properties, alongside high strain sensing accuracy and stable electrical output in flexible sensor applications. In conclusion, the PAA-Lignin-LM hydrogel holds excellent promise for wearable, flexible electronic products, introducing a novel approach for the high-value utilization of lignin.
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