| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Ahrens, Jens, 1978, et al.
(författare)
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Evaluation of Non-Spherical Scattering Bodies for Ambisonic Microphone Arrays
- 2022
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Ingår i: Proceedings of the AES International Conference. ; 2022-August, s. 58-66
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Konferensbidrag (refereegranskat)abstract
- The XMA was recently presented, which is a higher-order ambisonic microphone array with a non-spherical scattering body. The approach is compatible with the also recently presented equatorial microphone array so that also XMAs can be designed with the microphones distributed solely on a circumferential contour around the scattering body. This greatly reduces the required number of microphones compared to classical spherical microphone arrays that require the microphones to be distributed over the entire surface of the scatterer. The equatorial XMA has so far only been evaluated as a head-mounted array, i.e. with a human head as the baffle. Other form factors of a range of sizes are also of practical relevance, particularly those form factors of 360 cameras as these are capable of capturing a complete panoramic audio-visual experience from a first-person view when combined with an equatorial XMA. We present a set of simulations based on which we identify what spherical harmonic orders can be obtained with what accuracy for a set of convex scattering body geometries that are of relevance in the given context. We demonstrate that the shape of the body is not very critical, and even corners are possible. The main limitation is that small bodies do not allow for extracting higher orders at low frequencies.
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| 3. |
- Wang, Ziming, 1992, et al.
(författare)
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A Collaborative System of Flying and Ground Robots with Universal Physical Coupling Interface (PCI) and the Potential Interactive Applications
- 2022
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Ingår i: CHI EA '22: Extended Abstracts of the 2022 CHI Conference on Human Factors in Computing Systems. - New York, NY, USA : ACM. - 9781450391566
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Konferensbidrag (refereegranskat)abstract
- Flying and ground robots complement each other in terms of their advantages and disadvantages. We propose a collaborative system combining flying and ground robots, using a universal physical coupling interface (PCI) that allows for momentary connections and disconnections between multiple robots/devices. The proposed system may better utilize the complementary advantages of both flying and ground robots. We also describe various potential scenarios where such a system could be of benefit to interact with humans-namely, remote field works and rescue missions, transportation, healthcare, and education. Finally, we discuss the opportunities and challenges of such systems and consider deeper questions which should be studied in future work.
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| 4. |
- Wang, Ziming, 1992, et al.
(författare)
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The Effects of Natural Sounds and Proxemic Distances on the Perception of a Noisy Domestic Flying Robot
- 2023
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Ingår i: ACM Transactions on Human-Robot Interaction. - : Association for Computing Machinery (ACM). - 2573-9522. ; 12:4, s. 1-32
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Tidskriftsartikel (refereegranskat)abstract
- When flying robots are used in close-range interaction with humans, the noise they generate, also called consequential sound, is a critical parameter for user acceptance. We conjecture that there is a benefit in adding natural sounds to noisy domestic drones. To test our hypothesis experimentally, we carried out a mixed-methods research study (N=56) on reported user perception of a sonified domestic flying robot with three sound conditions at three distances. The natural sounds studied were respectively added to the robot’s inherent noises during flying; namely a birdsong and a rain sound, plus a control condition of no added sound. The distances studied were set according to proxemics; namely near, middle, and far. Our results show that adding birdsong or rain sound affects the participants’ perceptions, and the proxemic distances play a nonnegligible role. For instance, we found that participants liked the bird condition the most when the drone was at far, while they disliked the same sound the most when at near. We also found that participants’ perceptions strongly depended on their associations and interpretations deriving from previous experience. We derived six concrete design recommendations.
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