| 1. |
- Hamberg, Anders, et al.
(författare)
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C-terminal ladder sequencing of peptides using an alternative nucleophile in carboxypeptidase Y digests
- 2006
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Ingår i: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 357:2, s. 167-172
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Tidskriftsartikel (refereegranskat)abstract
- A method for improved sequence coverage in C-terminal sequencing of peptides, based on carboxypeptidase digestion, is described. In conventional carboxypeptidase digestions, the peptide substrate is usually extensively degraded and a full amino acid sequence cannot be obtained due to the lack of a complete peptide ladder. In the presented method, a protecting group is introduced at the C terminus of a fraction of the peptide fragments formed in the digest, and thereby further degradation of the C-terminally modified peptides are slowed down. The protecting group was attached to the C-terminal amino acid through a carboxypeptidase-catalyzed reaction with an alternative nucleophile, 2-pyridylmethylamine, added to the aqueous digestion buffer. Six peptides were digested by carboxypeptidase Y with and without 2-pyridylmethylamine present in the digest buffer, and the resulting fragments subsequently were analyzed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Comparison of the two digestion methods showed that the probability of successful ladder sequencing increased, by more than 50% using 2-pyridylmethylamine as a competing nucleophile in carboxypeptidase Y digests.
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| 2. |
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| 3. |
- Abgrall, N., et al.
(författare)
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The large enriched germanium experiment for neutrinoless double beta decay (LEGEND)
- 2017
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Ingår i: AIP Conference Proceedings. - : Author(s). - 1551-7616 .- 0094-243X. ; 1894
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Konferensbidrag (refereegranskat)abstract
- The observation of neutrinoless double-beta decay (0νββ) would show that lepton number is violated, reveal that neu-trinos are Majorana particles, and provide information on neutrino mass. A discovery-capable experiment covering the inverted ordering region, with effective Majorana neutrino masses of 15 - 50 meV, will require a tonne-scale experiment with excellent energy resolution and extremely low backgrounds, at the level of ∼0.1 count /(FWHM·t·yr) in the region of the signal. The current generation 76Ge experiments GERDA and the Majorana Demonstrator, utilizing high purity Germanium detectors with an intrinsic energy resolution of 0.12%, have achieved the lowest backgrounds by over an order of magnitude in the 0νββ signal region of all 0νββ experiments. Building on this success, the LEGEND collaboration has been formed to pursue a tonne-scale 76Ge experiment. The collaboration aims to develop a phased 0νββ experimental program with discovery potential at a half-life approaching or at 1028 years, using existing resources as appropriate to expedite physics results.
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| 4. |
- Abou Nada, Fahd Jouda, et al.
(författare)
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A first application of thermographic phosphors in a marine two-stroke diesel engine for surface temperature measurement
- 2014
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Ingår i: Proceedings of the ASME 2014 Internal Combustion Engine Division Fall Technical Conference (ICEF2014). ; , s. 001-001
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Konferensbidrag (refereegranskat)abstract
- Phosphor thermometry is applied for the first time in a large-bore two-stroke diesel engine. The work proves the practicality of phosphor thermometry in large-bore engines. The experiments were conducted on the MAN 4T50ME-X marine research engine equipped with an optical cylinder head. By employing a thin surface coating of CdWO4 phosphor, cycle resolved temperature measurements of the cylinder wall were obtained. Motored and fired engine operations were tested at engine loads covering the low and medium engine load range. Phosphor thermometry proved to be successful in retrieving the temperature with standard deviations ranging around 1-8 K. Experimental considerations like detector linearity, coating thickness and an automated phosphor calibration routine will be addressed.
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| 5. |
- Boström, Magnus, 1981-
(författare)
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Creating clarity and managing complexity through co-operation and communication : The case of Swedish icebreaker operations
- 2020
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Sea transportation is vital for the global economy, and the amount of seaborne trade is expected to increase in the future. In some areas, icebreakers are necessary for maintaining open shipping lanes all-year round and ensuring safe navigation. Vessels operating in ice are exposed to harsh environmental factors such as severe weather and heavy ice, and when external forces become too strong vessels will depend on icebreaker assistance. However, successful icebreaker operations require the icebreaker to operate in close vicinity to the assisted vessel to break the ice, which in turn increases the risk of collision.There are many factors which make icebreaker operations complex. The aim of this thesis is to use work organization, operational safety, and interpersonal communication as three lenses to describe and analyse the complexity of icebreaker operations, and its implications for practice. To thoroughly investigate this complexity, data are drawn from numerous sources; semi-structured interviews, a questionnaire, and a substantial amount of recorded authentic communication all provide complementary insights.The results show that the icebreaker performs a multitude of tasks directly concerned with icebreaking, e.g. directing and physically assisting other vessels, but that these tasks indirectly rely on interpersonal interaction and communication. A number of conflicting constraints add to the complexity. For example, harsh winter conditions impede vessels’ independent navigation in ice, while offering icebreaker crews opportunities to practice and maintain important skills. Furthermore, it was shown that language skills and communication play an important role in upholding the operational safety. However, closed-loop communication is not always used as intended, a deviation from intended communication protocol with potential to increase the risk of misunderstandings.This thesis suggests that safety and efficiency of winter navigation can be enhanced by making better use of existing technology and data; by examining the past track of other vessels, e.g. via AIS, finding suitable ice tracks will be made easier. Another implication concerning communication is that training institutes should emphasize the logic behind standardized communication protocols rather than focusing on standard phrases, i.e. facilitating means for advanced English speakers to adapt their communication style. That way, novice and advanced speakers could find common ground.
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| 6. |
- Engström, Carl-Johan, et al.
(författare)
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ÖP-RUP : från svag länk till plattform för utvecklingskraft
- 2010
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Rapport (populärvet., debatt m.m.)abstract
- Sverige genomgår en samhällsutveckling som ställer nya krav på samhällets organisation och funktion. I takt med att arbetsmarknadsregionerna växer och människors vardagliga livsrum vidgas, ökar behovet av en samhällsplanering som hanterar dessa frågor i ett regionalt perspektiv. Kommunerna har traditionellt haft den centrala rollen i samhällsplaneringen med ansvar för bebyggelseutveckling, offentligt finansierade vård- och omsorgstjänster, skola. Med de uppgifter som lagts på regionala organ får frågor som daglig pendling, arbetsmarknadens utveckling, etc. däremot sin behandling där. En kostnadseffektiv och ur medborgarsynpunkt ändamålsenlig organisering av skattefinansierade uppgifter och en hållbar samhällsutveckling är därmed beroende av att de kommunala och regionala nivåerna kan samspela. I samtliga administrativa regioner finns idag regionala utvecklingsprogram (RUP). Och inom kommunerna har översiktsplanens (ÖP) strategiska roll för kommunens långsiktiga utvecklingsambitioner ökat. I PBL- respektive Ansvarsutredningen har betydelsen av en bättre funktionell planering lyfts fram. Erfarenheterna visar emellertid att samspelet mellan ÖP och RUP är relativt outvecklat. Sveriges Kommuner och Landsting (SKL) har därför beslutat uppdra åt Kungliga Tekniska Högskolan (KTH) att göra en kunskapsöversikt som visar hur samhällsplaneringen kan ta till vara på utvecklingsmöjligheterna. Arbetsgruppen har bestått av Carl-Johan Engström, Charlotta Fredriksson och Anna Hult. Gruppen står för studien och dess slutsatser. Arbetet har fokuserat på att lyfta fram goda exempel. Processer där man har lyckats skapa gemensam handlingskraft i samspel mellan kommun och region, och likaså intressanta specifika utvecklingsstrategier utifrån lokala och/eller regionala styrkor och svagheter. Med dessa exempel som utgångspunkt förs en diskussion mot bakgrund av andra dokumenterade erfarenheter och forskningsresultat som tillsammans kan ge underlag och idéer för att skapa en bättre dialog mellan kommun och region som grund för strategisk utveckling och för samspelet med staten kring infrastruktursatsningar och regional utvecklingsplanering. En tjänstemannagrupp inom Sveriges Kommuner och Landsting har följt arbetet med kunskapsöversikten. Kontaktperson har varit Carmita Lundin.
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| 7. |
- Escher, Cecilia, et al.
(författare)
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Method matters: impact of in-scenario instruction on simulation-based teamwork training
- 2017
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Ingår i: Advances in Simulation. - : Springer Science and Business Media LLC. - 2059-0628 .- 2364-3277. ; 2:25
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Tidskriftsartikel (refereegranskat)abstract
- Background: The rationale for introducing full-scale patient simulators in training to improve patient safety is to recreate clinical situations in a realistic setting. Although high-fidelity simulators mimic a wide range of human features, simulators differ from the body of a sick patient. The gap between the simulator and the human body implies a need for facilitators to provide information to help participants understand scenarios. The authors aimed at describing different methods that facilitators in our dataset used to provide such extra scenario information and how the different methods to convey information affected how scenarios played out. Method: A descriptive qualitative study was conducted to examine the variation of methods to deliver extra scenario information to participants. A multistage approach was employed. The authors selected film clips from a shared database of 31 scenarios from three participating simulation centers. A multidisciplinary research team performed a collaborative analysis of representative film clips focusing on the interplay between participants, facilitators and the physical environment. After that the entire material was revisited to further examine and elaborate the initial findings. Results: The material displayed four distinct methods for facilitators to convey information to participants in simulation based teamwork training. The choice of method had impact on the participating teams regarding flow of work, tempo and team communication. Facilitators’ close access to the teams’ activities when present in the simulation suite, either embodied or disembodied in the simulation, facilitated the timing for providing information, which was critical for maintaining the flow of activities and learner engagement in the scenario. The mediation of information by a loudspeaker or an earpiece from the adjacent operator room could be disturbing for team communication when information was not well timed. Conclusions: In-scenario instruction is an essential component of simulation based teamwork training that has been largely overlooked in previous research. The ways in which facilitators convey information about the simulated patient have the potential to shape the simulation activities and thereby serve different learning goals. Although immediate timing to maintain an adequate tempo is necessary for professionals to engage in training of acute situations, novices may gain from a slower tempo to train complex clinical team tasks systematically.
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| 8. |
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| 9. |
- Fant, Eric Michael, 1754-1817
(författare)
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Dissertationis academicæ, de praefectura Naesgardensi Dalekarliae, continuatio IV:ta, sistens paroeciam Grytnäs; quam cons. ampliss. ord. philosoph. in reg. acad. Upsaliensi, præside mag. Erico M. Fant, histor. prof. reg. et ord. S. R. S. M. pro gradu, publice ventilandam sistit Johannes Christoph. Hult, stipend. Nessel. Dalekarlus. In aud. Gust. maj. d. 24 Maji, 1782.
- 1782
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)
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| 10. |
- Garcia Gallego, Sandra, et al.
(författare)
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Fluoride-Promoted Esterification with Imidazolide-Activated Compounds : A Modular and Sustainable Approach to Dendrimers
- 2015
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Ingår i: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 54:8, s. 2416-2419
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Tidskriftsartikel (refereegranskat)abstract
- Based on the growing demand for facile and sustainable synthetic methods to structurally perfect polymers, we herein describe a significant improvement of esterification reactions capitalizing on 1,1-carbonyldiimidazole (CDI). Cesium fluoride was shown to be an essential catalyst for these reactions to reach completion. This approach was successfully applied to the synthesis of structurally flawless and highly functional polyester dendrimers employing traditional and accelerated growth strategies. A sixth generation bis-MPA dendrimer with a molecular weight of 22.080Da and 192 peripheral hydroxy groups was isolated in less than one day of total reaction time. Large quantities of dendrimerswere obtained in high yields (>90%) using simple purification steps under sustainable conditions. The fluoride-promoted esterification (FPE) via imidazolide-activated compounds is wide in scope and constitutes a potentially new approach toward functional polymers and other materials.
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