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- Jakobsen, Lis, et al.
(författare)
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Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods
- 2011
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Ingår i: EMBO Journal. - : Wiley. - 0261-4189 .- 1460-2075. ; 30:8, s. 1520-1535
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Tidskriftsartikel (refereegranskat)abstract
- Centrosomes in animal cells are dynamic organelles with a proteinaceous matrix of pericentriolar material assembled around a pair of centrioles. They organize the microtubule cytoskeleton and the mitotic spindle apparatus. Mature centrioles are essential for biogenesis of primary cilia that mediate key signalling events. Despite recent advances, the molecular basis for the plethora of processes coordinated by centrosomes is not fully understood. We have combined protein identification and localization, using PCP-SILAC mass spectrometry, BAC transgeneOmics, and antibodies to define the constituents of human centrosomes. From a background of non-specific proteins, we distinguished 126 known and 40 candidate centrosomal proteins, of which 22 were confirmed as novel components. An antibody screen covering 4000 genes revealed an additional 113 candidates. We illustrate the power of our methods by identifying a novel set of five proteins preferentially associated with mother or daughter centrioles, comprising genes implicated in cell polarity. Pulsed labelling demonstrates a remarkable variation in the stability of centrosomal protein complexes. These spatiotemporal proteomics data provide leads to the further functional characterization of centrosomal proteins.
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- Kjellberg, Anders, et al.
(författare)
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Sweden: Can the Model Survive?
- 1992
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Ingår i: Industrial Relations in the New Europe. - 0631185925 - 0631185933 ; , s. 88-142
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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- Kjellberg, Anders, et al.
(författare)
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Sweden: Restoring the Model?
- 1998
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Ingår i: Changing Industrial Relations in Europe (Blackwell Business). - 0631205519 ; , s. 74-117
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Since the mid-1960s Swedish industrial relations have undergone such fundamental changes that the very existence of a 'Swedish model' is now questioned. Nevertheless, given the continuing similarities between Nordic countries, and the differences between them as a group and other European countries, the notion of a 'Nordic model' of industrial relations still has descriptive and analytic value. First, therefore, the broad outlines of the 'Nordic model' will be presented. The main focus of the chapter, however, is on Swedish industrial relations: the roots of the 'historic compromise' between capital and labour in the 1930s, and the erosion of the Swedish model of 'self-regulation' and centralised bargaining by increased state intervention and pressures to decentralisation.
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- Skogs, Marie, et al.
(författare)
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Antibody Validation in Bioimaging Applications Based on Endogenous Expression of Tagged Proteins
- 2017
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 16:1, s. 147-155
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Tidskriftsartikel (refereegranskat)abstract
- Antibodies are indispensible research tools, yet the scientific community has not adopted standardized procedures to validate their specificity. Here we present a strategy to systematically validate antibodies for immunofluorescence (IF) applications using gene tagging. We have assessed the on- and off-target binding capabilities of 197 antibodies using 108 cell lines expressing EGFP-tagged target proteins at endogenous levels. Furthermore, we assessed batch-to-batch effects for 35 target proteins, showing that both the on- and off-target binding patterns vary significantly between antibody batches and that the proposed strategy serves as a reliable procedure for ensuring reproducibility upon production of new antibody batches. In summary, we present a systematic scheme for antibody validation in IF applications using endogenous expression of tagged proteins. This is an important step toward a reproducible approach for context- and application-specific antibody validation and improved reliability of antibody-based experiments and research data.
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- Wayne, Greg, et al.
(författare)
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Principles of Systems Biology, No. 11
- 2016
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Ingår i: CELL SYSTEMS. - : CELL PRESS. - 2405-4712. ; 3:5, s. 406-410
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Tidskriftsartikel (refereegranskat)abstract
- This month: AI that learns patterns and facts, new protein-RNA and protein-protein relationships, engineering signaling and metabolism, and more variants of Cas9.
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