| 1. |
- Boele, Joost, et al.
(författare)
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PAPD5-mediated 3' adenylation and subsequent degradation of miR-21 is disrupted in proliferative disease.
- 2014
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Ingår i: Proceedings of the National Academy of Sciences. - : Proceedings of the National Academy of Sciences. - 1091-6490 .- 0027-8424. ; 111:31, s. 11467-11472
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Tidskriftsartikel (refereegranskat)abstract
- Next-generation sequencing experiments have shown that microRNAs (miRNAs) are expressed in many different isoforms (isomiRs), whose biological relevance is often unclear. We found that mature miR-21, the most widely researched miRNA because of its importance in human disease, is produced in two prevalent isomiR forms that differ by 1 nt at their 3' end, and moreover that the 3' end of miR-21 is posttranscriptionally adenylated by the noncanonical poly(A) polymerase PAPD5. PAPD5 knockdown caused an increase in the miR-21 expression level, suggesting that PAPD5-mediated adenylation of miR-21 leads to its degradation. Exoribonuclease knockdown experiments followed by small-RNA sequencing suggested that PARN degrades miR-21 in the 3'-to-5' direction. In accordance with this model, microarray expression profiling demonstrated that PAPD5 knockdown results in a down-regulation of miR-21 target mRNAs. We found that disruption of the miR-21 adenylation and degradation pathway is a general feature in tumors across a wide range of tissues, as evidenced by data from The Cancer Genome Atlas, as well as in the noncancerous proliferative disease psoriasis. We conclude that PAPD5 and PARN mediate degradation of oncogenic miRNA miR-21 through a tailing and trimming process, and that this pathway is disrupted in cancer and other proliferative diseases.
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| 2. |
- Marmolejo-Ramos, Fernando, et al.
(författare)
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Your Face and Moves Seem Happier When I Smile Facial Action Influences the Perception of Emotional Faces and Biological Motion Stimuli
- 2020
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Ingår i: Experimental psychology (Göttingen). - : Hogrefe Publishing Group. - 1618-3169 .- 2190-5142. ; 67:1, s. 14-22
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Tidskriftsartikel (refereegranskat)abstract
- In this experiment, we replicated the effect of muscle engagement on perception such that the recognition of another's facial expressions was biased by the observer's facial muscular activity (Blaesi & Wilson, 2010). We extended this replication to show that such a modulatory effect is also observed for the recognition of dynamic bodily expressions. Via a multitab and within-subjects approach, we investigated the emotion recognition of point-tight biological walkers, along with that of morphed face stimuli, white subjects were or were not holding a pen in their teeth. Under the pen-in-the-teeth condition, participants tended to tower their threshold of perception of happy expressions in facial stimuli compared to the no-pen condition, thus replicating the experiment by Blaesi and Wilson (2010). A similar effect was found for the biological motion stimuli such that participants Lowered their threshold to perceive happy walkers in the pen-in-the-teeth condition compared to the no-pen condition. This pattern of results was also found in a second experiment in which the no-pen condition was replaced by a situation in which participants held a pen in their lips (pen-in-tips condition). These results suggested that facial muscular activity alters the recognition of not only facial expressions but also bodily expressions.
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