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- Isaksson, Jessica, 1975
(författare)
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A tentative approach towards handling users’ haptic product experiences
- 2007
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Ingår i: Ergonomics for a future: NES, Nordic Ergonomic society, 2007. - 9789197152129 ; , s. 212-
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Konferensbidrag (refereegranskat)abstract
- Designing products for tomorrow is about creating user experiences. This requires a holistic view on the user, the product and their interaction, which should involve all senses for example. The focus here is on the first steps towards a method on how to obtain information on users’ haptic experiences. The basic stages of the method are presented, along with a case study. The method has shown to be useful to retrieve different haptic experiences of products described in terms of adjectives. Further developed it could expectantly support attaining specific haptic product properties related to general experiences.
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| 2. |
- Isaksson, Jessica, 1975, et al.
(författare)
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Evaluation of a haptic interface for in-vehicle systems
- 2003
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Ingår i: Proceedings of the International Ergonomics Association XVth Triennial Congress – Ergonomics in the digital age, Seoul, Korea, August 24-29, 2003.
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Konferensbidrag (refereegranskat)
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| 3. |
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| 4. |
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| 5. |
- Isaksson, Jessica, 1975, et al.
(författare)
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Meaning through doing
- 2007
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Ingår i: Sixth Nordcode Seminar & Workshop, Design Semiotics in Use.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- In Gibson’s theory of perception, an organism directly perceives the value of the environment through affordances. By affordance, Gibson means the opportunities or possibilities of nature, which require the act of information pickup. Within design theory, however, there is a strong tendency towards separating perceptual information of affordances and the affordance itself. Combining theoretical discussion with an empirical case study of a medical device, we suggest there is untapped value in the notion of direct perception and argue that there is meaning through doing. Looking at the role of affordances over time, instead of a person’s first exposure to a product necessitates sensitivity toward enskilment and how people create meaning through the use of products.
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| 6. |
- Isaksson, Jessica, 1975
(författare)
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Towards mapping and applying haptics in user-product interaction and product development: An empirical and theoretical approach
- 2005
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Developing products for user-product interaction consists of many dimensions to consider for a product developer. The situations that occur when users start to explore and interact with a product are very complex; both mental and physical actions are engaged and our senses perceive and interpret the situations. The haptic sense refers to our sense of touch in a broad context, and comprises what people perceive in the skin, muscles, joints and tendons, as in user-product interaction to a high degree. The perceptions and interpretations obtained by the haptic sense contribute to how the product is experienced by the users in the range from specific product properties, such as weight etc., to more subjective parameters such as the overall quality impression, as well as how congruently the product conveys the information to the users senses. In the scientific and industrial area of product development, the haptic sense has not been treated in the development of products to the same extent as vision, for example, despite its importance. This research projects main purpose is to understand and describe haptics and its relation to user-product interaction, and further to examine how it can be systematically managed and applied in product development work. In this thesis, the theoretical and empirical results produced so far have been interwoven into a framework. The aim with the framework is to provide a foundation for describing the importance of haptics in user-product interaction and product development. This can be useful for both science and industry. The research approach applied in this project has been a constant iteration between theoretical and empirical reasoning. Design research methodology (DRM) has been used to support the empirical work and systematically structure it. The criterion well-thought-out haptic properties in products will improve user-product interaction has served as the starting-point, and the rest of the work, research questions and appropriate research methods were derived from that statement. Since haptics in relation to users and products, from the perspective presented in the research project, is a relatively new topic, this thesis first of all contributes new important information in the area of user-product interaction and product development. The haptic sense is described and the implications it can have for user-product interactions are examined, since it is a significant ingredient to be well aware of when developing products. It is also shown that haptics in user-product interaction plays an important role for how users experience both specific product properties and subjective parameters such as how well a product expresses its properties and describes its intentions. All these aspects together affect the users overall impression of the products quality and potential. To implement haptics in product development, it has been found necessary to have supplementary information on the topic for industry and science. A common terminology is needed for all different professions that come into contact with haptics in one way or another in industry, so that they can communicate about all aspects of haptics. They need to keep a holistic view of the users and the products also because of the character of haptics, since specific product properties are as important as the overall impression of the product. Industry needs support in terms of guidelines, tools and methods that also must allow a holistic view. In order to systematically implement this in product development, an integrated product development process is recommended.
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| 7. |
- Olofsson, Jessica, 1975, et al.
(författare)
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Direct Access and Control of the Intracellular Solution Environment in Single Cells
- 2009
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 81:5, s. 1810-1818
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Tidskriftsartikel (refereegranskat)abstract
- Methods that can control and vary the solution environment around single cells are abundant. In contrast, methods that offer direct access to the intracellular proteome and genome in single cells with the control, flexibility, and convenience given by microfluidic methods are both scarce and in great demand. Here, we present such a method based on using a microfluidic device mounted on a programmable scanning stage and cells on-chip permeabilized by the pore-forming glycoside digitonin. We characterized the on-chip digitonin poration, as well as the solution exchange within cells. Intracellular solution exchange times vary with the dose of exposure to digitonin from less than a second to tens of seconds. Also, the degree of permeabilization obtained for cells treated with the same dose varies considerably, especially for low doses of digitonin exposure and low permeabilities. With the use of the presented setup, the degree of permeabilization can be measured during the permeabilization process, which allows for "on-line" optimization of the digitonin exposure time. Using this calibrated permeabilization method, we demonstrate the generation of intracellular oscillations, intracellular gradients, and the delivery of substrate to initiate enzymatic reactions in situ. This method holds the potential to screen and titrate intracellular receptors or enzymes or to generate intracellular oscillations, useful in the study of signaling pathways and oscillation decoding among other applications.
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| 8. |
- Olofsson, Jessica, 1975, et al.
(författare)
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Probing enzymatic activity inside single cells.
- 2013
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 85:21, s. 10126-33
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Tidskriftsartikel (refereegranskat)abstract
- We report a novel approach for determining the enzymatic activity within a single suspended cell. Using a steady-state microfluidic delivery device and timed exposure to the pore-forming agent digitonin, we controlled the plasma membrane permeation of individual NG108-15 cells. Mildly permeabilized cells (∼100 pores) were exposed to a series of concentrations of fluorescein diphosphate (FDP), a fluorogenic alkaline phosphatase substrate, with and without levamisole, an alkaline phosphatase inhibitor. We generated quantitative estimates for intracellular enzyme activity and were able to construct both dose-response and dose-inhibition curves at the single-cell level, resulting in an apparent Michaelis contant Km of 15.3 μM ± 1.02 (mean ± standard error of the mean (SEM), n = 16) and an inhibition constant Ki of 0.59 mM ± 0.07 (mean ± SEM, n = 14). Enzymatic activity could be monitored just 40 s after permeabilization, and five point dose-inhibition curves could be obtained within 150 s. This rapid approach offers a new methodology for characterizing enzyme activity within single cells.
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