| 1. |
- Islam, M. Shahidul, et al.
(author)
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Engineered beta-cells secreting dipeptidyl peptidase IV-resistant glucagon-like peptide-1 show enhanced glucose-responsiveness
- 2005
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In: Life Sciences. - : Elsevier BV. - 0024-3205 .- 1879-0631. ; 76:11, s. 1239-1248
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Journal article (peer-reviewed)abstract
- Type 2 diabetes is a polygenic disorder characterized by increased insulin resistance, and impaired insulin secretion leading to abnormalities of glucose and lipid metabolism. Reduced responsiveness of the beta-cells to glucose is a critical feature of this syndrome. Glucagon-like peptide 1, a product of the pro-glucagon gene makes beta-cells competent and has many other anti-diabetic properties. We speculated whether GLP-1-based gene therapy could be an approach for treatment of type 2 diabetes. We started with a clone of rat insulinoma cells (S4 cells), which showed reduced responsiveness to glucose in terms of insulin secretion. We transfected these cells with a plasmid encoding a mutated form of GLP-1 (GLP-1-Gly8), which is resistant to the degrading enzyme dipeptidyl-peptidase IV. Activity of secreted GLP-1-Gly8 was assayed using Chinese hamster lung fibroblasts (CHL) cells that expressed cloned GLP-1 receptor and that were transfected with CRE-Luc. Stable cell lines (Glipsulin cells) obtained by this means produced and stored immunoreactive GLP-1-Gly8. In addition to insulin, the Glipsulin cells secreted the GLP-1-Gly8. The secreted GLP-1-Gly8 was active as evidenced by the ability of the conditioned media to elevate cAMP levels in CHL cells expressing GLP-1 receptors. Glipsulin cells responded to glucose with a 6.8 fold increase in insulin secretion compared to a 2.2 fold increase in the control cells. Our results demonstrate that prolonged exposure to GLP-1-Gly8 secreted by increases glucose-responsiveness of these cells. We speculate that engineering GLP-1-Gly8 secretion by beta-cells is a potential gene therapeutic strategy to treat diabetes.
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| 2. |
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| 3. |
- Islam, M. Shahidul, et al.
(author)
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Is road-side fishpond water in Bangladesh safe for human use? An assessment using water quality indices
- 2022
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In: Environmental Challenges. - : Elsevier BV. - 2667-0100. ; 6
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Journal article (peer-reviewed)abstract
- Pond water is used in everyday life by many people in Bangladesh, however, without sufficient and reliable information regarding water quality and pollution status. For this reason, geospatial analysis and mapping of water quality indices such as metal (MI), contamination (Cd), and physicochemical water quality index (WQI) were assessed to improve the understanding of potential pollution sources. Samples were collected from twenty randomly selected ponds situated in Jashore Sadar Upazila, Bangladesh. Nineteen (19) water quality parameters were measured, including pH, temperature, EC, TDS, total suspended solids (TSS), chloride, alkalinity, total hardness, salinity, Fe, Mn, Pb, Cr, Cd, Co, Zn, Ag, Ni, and Cu. The average concentration of Fe, Mn, Pb, Cd, and Ag was much higher than recommended standards. The WQI ranged from 1.59-5.27, Cd from -0.19-18.28, and MI from 7.81-26.28. The spatial distribution of MI indicates that the south-western and south-eastern region of the study area are stands out with a very high pollution pressure. The spatial distribution of Cd, follows the same trend as for MI. A multitude of different types of pollution sources contributes to the high pollution load such as, municipal wastewater, leachate from landfills, small industry wastewater and stormwater, and agricultural runoff. The studied pond water is highly polluted and not suitable for household use and fish consumption.
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| 4. |
- Islam, M. Shahidul, et al.
(author)
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Multiple reuse of disposable insulin syringes in hospital
- 1990
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In: Bangladesh Medical Research Council bulletin. - 0377-9238. ; 16:2, s. 58-61
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Journal article (peer-reviewed)abstract
- In a prospective study disposable insulin syringes were repeatedly used for injecting insulin to 92 insulin-taking diabetic patients admitted in hospital with various illnesses. One separate syringe was used for each patient; the syringes were not washed or boiled and were not kept in spirit or in refrigerator during reuse. Syringes were flushed with air, needle recapped and replaced into their plastic cover. Number of injections made using one syringe was 2 to 120 (mean 31.3, SEM 2.3). For the patients' total insulin - taking period in hospital one disposable syringe per patient was enough in all but six cases. There were 2792 reinjections and incidence of infection of the injection site was zero.
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| 5. |
- Islam, M. Shahidul, et al.
(author)
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Rat insulinoma-derived pancreatic beta-cells express a functional leptin receptor that mediates a proliferative response
- 1997
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In: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 238:3, s. 851-855
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Journal article (peer-reviewed)abstract
- In addition to its interaction at hypothalamic sites to affect feeding and energy expenditure, leptin has been shown to exhibit a proliferative response in erythropoietic cells. The functional leptin receptor is also present in pancreatic islets and we now demonstrate that a commonly used clonal insulin secreting beta-cell line, RINm5F, expresses high levels of the Ob-Rb mRNA. Leptin causes an increase in tyrosine phosphorylation of a number of intracellular proteins and a dose related (10 nM-200 nM) increase in expression of the immediate-early gene, c-fos. This precedes a leptin induced proliferative response in serum-deprived RINm5F cells, which suggests that leptin might be involved in the complex regulation of proliferation of the pancreatic beta-cell.
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| 6. |
- Islam, M. Shahidul, et al.
(author)
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Spatial Distribution and Source Identification of Water Quality Parameters of an Industrial Seaport Riverbank Area in Bangladesh
- 2022
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In: Water (Switzerland). - : MDPI AG. - 2073-4441. ; 14:9
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Journal article (peer-reviewed)abstract
- The Pasur River is a vital reservoir of surface water in the Sundarbon area in Bangladesh. Mongla seaport is located on the bank of this river. Many industries and other commercial sectors situated in this port area are discharging waste into the river without proper treatment. For this reason, geospatial analysis and mapping of water pollutant distribution were performed to assess the physicochemical and toxicological situation in the study area. We used different water quality indices such as Metal Index (MI), Comprehensive Pollution Index (CPI), and Weighted Arithmetic Water Quality Index Method (WQI) to improve the understanding of pollution distribution and processes determining the quality of river water. Multivariate statistical methods were used to evaluate loads and sources of pollutants in the Pasur River system. The results indicate that the sources of contaminants are both geogenic and anthropogenic, including untreated or poorly treated wastewater from industries and urban domestic waste discharge. The concentration range of total suspended solid (TSS), chloride, iron (Fe), and manganese (Mn) were from 363.2 to 1482.7, 108.2 to 708.93, 1.13 to 2.75, and 0.19 to 1.41 mg/L, respectively, significantly exceeding the health-based guideline of WHO and Bangladeshi standards. The high Fe and Mn contents are contributions from geogenic and anthropogenic sources such as industrial waste and construction activities. The average pH value was 8.73, higher than the WHO and Bangladeshi standard limit. WQI (ranging from 391 to 1336), CPI (6.71 to 23.1), and MI (7.23 to 23.3) were very high and greatly exceeded standard limits indicating that the Pasur River water is highly polluted. The results of this study can be used as a first reference work for developing a surface water quality monitoring system and guide decisionmakers for priorities regarding wastewater treatment.
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| 7. |
- Islam, M. Shahidul, et al.
(author)
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Toxicity and source identification of pollutants in an urban river in Bangladesh
- 2023
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In: Environmental Earth Sciences. - : Springer Science and Business Media LLC. - 1866-6280 .- 1866-6299. ; 82:6
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Journal article (peer-reviewed)abstract
- Urban rivers in Asian developing countries are becoming increasingly polluted due to industrialization and lacking treatment of wastewater. We investigated toxicity and likely sources of pollutants for the urban Shitalakshaya River, Bangladesh. Physiochemical variables and heavy metals were examined in water and sediment of an urban river section in Narayanganj City. The spatial distribution of quality indices and cluster groups indicates that the river’s downstream urban-affected areas are the most contaminated. Water and sediment quality guidelines indicate that COD, TSS, Fe, Pb, Zn in water, and Pb, Co, Ni, Cu, Zn, and particularly Pb and Cu in sediment, pose a serious threat to the aquatic ecosystem and human health in the area. Correlation, principal component (PCA), and cluster analysis (CA) indicate that the sources of Mn and Cd are geogenic, COD, TSS, Pb, Zn, Cu anthropogenic, and Fe, Ni, Co both geogenic and anthropogenic. The main anthropogenic pollution sources of the study area are municipal and industrial wastewater, boat and car traffic, runoff from agricultural areas, and stormwater runoff.
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| 8. |
- Webb, Dominic-Luc, et al.
(author)
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Insulin exocytosis and glucose-mediated increase in cytoplasmic free Ca2+ concentration in the pancreatic beta-cell are independent of cyclic ADP-ribose
- 1996
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In: Journal of Biological Chemistry. - : Elsevier BV. - 0021-9258 .- 1083-351X. ; 271:32, s. 19074-19079
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Journal article (peer-reviewed)abstract
- Stimulation of pancreatic beta-cells by glucose gives rise to an increase in the cytoplasmic free calcium concentration ([Ca2+]i) and exocytosis of insulin. Cyclic adenosine 5'-diphosphate ribose (cADPR), a metabolite of beta-NAD+, has been reported to increase [Ca2+]i in pancreatic beta-cells by releasing Ca2+ from inositol 1,4,5-trisphosphate-insensitive intracellular stores. In the present study, we have examined the role of cADPR in glucose-mediated increases in [Ca2+]i and insulin exocytosis. Dispersed ob/ob mouse beta-cell aggregates were either pressure microinjected with fura-2 salt or loaded with fura-2 acetoxymethyl ester, and [Ca2+]i was monitored by microfluorimetry. Microinjection of beta-NAD+ into fura-2-loaded beta-cells did not increase [Ca2+]i nor did it alter the cells' subsequent [Ca2+]i response to glucose. Cells microinjected with the cADPR antagonist 8NH2-cADPR increased [Ca2+]i in response to glucose equally well as those injected with cADPR. Finally, the ability of cADPR to promote exocytosis of insulin in electropermeabilized beta-cells was investigated. cADPR on its own did not increase insulin secretion nor did it potentiate Ca2+-induced insulin secretion. We conclude that cADPR neither plays a significant role in glucose-mediated increases in [Ca2+]i nor interacts directly with the molecular mechanisms regulating exocytosis of insulin in normal pancreatic beta-cells.
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| 9. |
- Azad Khan, A. K., et al.
(author)
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Predicting first professional M.B.B.S. examination score from the results of H.S.C. examination
- 1980
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In: Bangladesh Medical Research Council Bulletin. - 0377-9238. ; 6:2, s. 61-72
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Journal article (peer-reviewed)abstract
- In a retrospective study carried out in Chittagong Medical College the results of HSC and First-Professional MBBS examination of 725 students were analysed. It was concluded from the analysis that if standard of HSC examination is well maintained the results of the said examination correlates well with students' performance in the First Professional MBBS examination. An analysis of admission into Chittagong Medical College in past 20 academic sessions has been made which shows that an increasing number of students securing high marks in HSC are being admitted into Medical College during recent years. The marking standard of HSC examination has however remained fairly uniform over the years except during early post-liberation period.
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| 10. |
- Azad Khan, A. K., et al.
(author)
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Stool findings in "chronic dysentery"
- 1981
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In: Bangladesh Medical Research Council Bulletin. - 0377-9238. ; 7:1, s. 7-11
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Journal article (peer-reviewed)abstract
- Faecal samples from 63 subjects with self-diagnosis of "chronic dysentery" and 50 control subjects were examined under light microscope. Vegetative form of E.h. was not detected in any of them. E.h. cyst was found in 6.3% of the "chronic dysentery" subjects and in 16% of the control subjects. E. coli and Giardia were also detected less often in the "chronic dysentery" than the control subjects. Lower incidence of protozoa namely E.h. cyst, E. coli cyst or Giardia in the former group was probably due to frequent intake of antiamoebic agents which are also effective against other intestinal protozoa. Incidence of Ascaris, hookworm, and Trichuris was not appreciably different in the two groups. More subjects in the "chronic dysentery" group had normal stool findings (31%) as compared to the control subjects (16%). It has been inferred that E.h. infection is not the cause of symptoms of "chronic dysentery".
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