| 1. |
- Ozawa, Takayuki, et al.
(författare)
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Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice
- 2021
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Ingår i: iScience. - : Elsevier BV. - 2589-0042. ; 24:5
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Tidskriftsartikel (refereegranskat)abstract
- Targeting the signaling pathway of growth differentiation factor 8 (GDF8), also known as myostatin, has been regarded as a promising strategy to increase muscle mass in the elderly and in patients. Accumulating evidence in animal models and clinical trials has indicated that a rational approach is to inhibit a limited number of transforming growth factor beta (TGF-beta) family ligands, including GDF8 and activin A, without affecting other members. Here, we focused on one of the endogenous antagonists against TGF-beta family ligands, follistatin-like 3 (FSTL3), which mainly binds and neutralizes activins, GDF8, and GDF11. Although bivalent human FSTL3 Fc-fusion protein was rapidly cleared from mouse circulation similar to follistatin (FST)-Fc, monovalent FSTL3-Fc (mono-FSTL3-Fc) generated with the knobs-into-holes technology exhibited longer serum half-life. Systemic administration ofmono-FSTL3-Fc in mice induced muscle fiber hypertrophy and increased muscle mass in vivo. Our results indicate that the monovalent FSTL3-based therapy overcomes the difficulties of current anti-GDF8 therapies.
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| 2. |
- Reina, Guido, et al.
(författare)
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The moving target of visualization software for an increasingly complex world
- 2020
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Ingår i: Computers & graphics. - : PERGAMON-ELSEVIER SCIENCE LTD. - 0097-8493 .- 1873-7684. ; 87, s. 12-29
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Tidskriftsartikel (refereegranskat)abstract
- Visualization has evolved into a mature scientific field and it has also become widely accepted as a standard approach in diverse fields, including physics, life sciences, and business intelligence. However, despite its successful development, there are still many open research questions that require customized implementations in order to explore and establish concepts, and to perform experiments and take measurements. Many methods and tools have been developed and published but most are stand-alone prototypes and have not reached a mature state that can be used in a reliable manner by collaborating domain scientists or a wider audience. In this study, we discuss the challenges, solutions, and open research questions that affect the development of sophisticated, relevant, and novel scientific visualization solutions with minimum overheads. We summarize and discuss the results of a recent National Institute of Informatics Shonan seminar on these topics. (C) 2020 Elsevier Ltd. All rights reserved.
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