| 1. |
- Hansen, Nils, et al.
(författare)
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SOCS2 is dispensable for BCR/ABL1-induced chronic myeloid leukemia-like disease and for normal hematopoietic stem cell function.
- 2013
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Ingår i: Leukemia. - : Springer Science and Business Media LLC. - 1476-5551 .- 0887-6924. ; 27, s. 130-135
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Tidskriftsartikel (refereegranskat)abstract
- Suppressor of cytokine signaling 2 (SOCS2) is known as a feedback inhibitor of cytokine signaling and is highly expressed in primary bone marrow (BM) cells from patients with chronic myeloid leukemia (CML). However, it has not been established whether SOCS2 is involved in CML, caused by the BCR/ABL1 fusion gene, or important for normal hematopoietic stem cell (HSC) function. In this study, we demonstrate that although Socs2 was found to be preferentially expressed in long-term HSCs, Socs2-deficient HSCs were indistinguishable from wild-type HSCs when challenged in competitive BM transplantation experiments. Furthermore, by using a retroviral BCR/ABL1-induced mouse model of CML, we demonstrate that SOCS2 is dispensable for the induction and propagation of the disease, suggesting that the SOCS2-mediated feedback regulation of the JAK/STAT pathway is deficient in BCR/ABL1-induced CML.Leukemia advance online publication, 24 July 2012; doi:10.1038/leu.2012.169.
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| 2. |
- Lee, S. W., et al.
(författare)
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Sol-gel integrated protein microarray for high-resolution signal readout of psa (prostate specific antigen) in clinical samples
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; 2, s. 800-802
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Konferensbidrag (refereegranskat)abstract
- In this study, we demonstrate a new protein microarray technology for highly sensitive detection of PSA (prostate caser specific antigen) in scrum samples. Using the optically active sol-gel nanocomposites, which can hold capturing probes in native and surface morphology tailored porous silicon, purified PSA (Prostate Specific Antigen) in human female serum was assessed by FlTC-antiPSA in broad dynamic range of the sandwich (1 pg/mL to Ing/mL). Additionally, we tested reverse phase assay using our developed system, which purified PSA imbedded in sol-gel and FITC labeled its counter antibody was accessed. Dynamic range was 60 fg/mL to 6ng/mL. Our concept can allow the measurement oflow amount of PSA at pg/ml range and thus, it is possible to do relative quantification for marker protein as well.
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| 3. |
- Lögdberg, Sara, et al.
(författare)
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Further insights into methane and higher hydrocarbons formation over cobalt-based catalysts with γ-Al2O3, α-Al2O3 and TiO2 as support materials
- 2017
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Ingår i: Journal of Catalysis. - : Academic Press. - 0021-9517 .- 1090-2694. ; 352, s. 515-531
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Tidskriftsartikel (refereegranskat)abstract
- A range of cobalt-based catalysts varying in Co loading and prepared by incipient wetness impregnation of traditional support materials (γ-Al2O3, α-Al2O3 and TiO2), have been studied in the Fischer-Tropsch reaction at industrially relevant process conditions (483 K, 20 bar, H2/CO = 2.1). A high selectivity to C5+ hydrocarbons (SC5+) is to a great extent connected with a high site activity, but not exclusively. We propose that the ratio of monomer-production rate to C–C coupling rate of a catalyst determines chain-growth probability by means of governing the coverage of the monomer on the cobalt surface. We speculate that this ratio depends on e.g. shape, strain and size of the Co crystallites and, therefore, is highly dependent on the choice of support material. No general relationship between Co particle size and SC5+ is found, but individual correlations exist for each support material. Within each support material, there are indications of negative correlations between the chain-growth probability of the C1 ∗ surface intermediate (αC1) and the higher αCn values. This can be rationalized by assuming that the majority of methane is formed by a different mechanism, separate from chain growth, but connected with chain growth through a common carbon pool. We propose that the monomers and the majority of methane are produced at sites different from the ones involved in chain growth. There is no general correlation between αC1 and SC5+ for catalysts with different support materials, possibly due to small differences in cobalt surface coverage of hydrogen. For the TiO2-supported catalysts, a dramatically increased αC1 value observed for catalysts with Co particles smaller than approx. 15 nm, is probably associated with strong metal-support interactions (SMSI). This phenomenon apparently limits the effect (reduction) on the SC5+ when moving toward smaller Co particles.
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| 4. |
- Montes, V., et al.
(författare)
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Preparation and characterization of Pt-modified Co-based catalysts through the microemulsion technique : Preliminary results on the Fischer-Tropsch synthesis
- 2014
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Ingår i: Catalysis Today. - : Elsevier BV. - 0920-5861 .- 1873-4308. ; 223, s. 66-75
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Tidskriftsartikel (refereegranskat)abstract
- The influence of the addition of small amounts of platinum (0.1-0.25% wt) to cobalt-based systems on Fischer-Tropsch synthesis was investigated. The solids were synthesized through microemulsion technique using TiO2 as the support. The best catalytic performance was achieved using Synperonic 13/6.5 as the surfactant. In all cases, the presence of platinum led to an increase in CO conversion which could be ascribed to the promotion of cobalt reducibility as evidenced by XPS. Moreover, the simultaneous reduction of cobalt and platinum precursors during synthetic procedure (ME1) was preferable to the consecutive one (ME2) probably as a result of a better Co-Pt interaction in the former case, as evidenced by TPR. TPR, Raman and XPS data also suggested that not only the presence of Co-0 but also the appearance of Co-TiO2 interactions favor the catalytic performance and that in general those interactions are stronger for ME1 solids.
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| 5. |
- Montes, V., et al.
(författare)
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Selective transformation of glycerol into 1,2-propanediol on several Pt/ZnO solids : Further insight into the role and origin of catalyst acidity
- 2015
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Ingår i: Catalysis Today. - : Elsevier BV. - 0920-5861 .- 1873-4308. ; 257, s. 246-258
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Tidskriftsartikel (refereegranskat)abstract
- Microemulsion technique allowed us to synthesize different ZnO solids with similar particle sizes and textural properties. Platinum was subsequently incorporated by deposition-precipitation and impregnation methods and solids tested for glycerol selective transformation into 1,2-PDO. Incorporation of platinum led to the creation of new (mainly Lewis) acid sites. A good correlation between conversion and acidity of Pt/ZnO solids was obtained. Interestingly, despite exhibiting some acidity, supports alone were inactive in the process which evidenced the role of the metal in dehydration of glycerol into acetol. Furthermore, as the reaction proceeded some chlorine coming from the precursor (H2PtCl6) was leached which led to the disappearance of the strongest acid sites, associated to side reactions (catalytic cracking) thus resulting in an increase in selectivity to 1,2-PDO. Eventual formation of Pt-Zn alloy upon reduction of the systems at ca. 400 degrees C was beneficial to 1,2-PDO selectivity.
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| 6. |
- Montes, V., et al.
(författare)
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Synthesis of different ZnO-supported metal systems through microemulsion technique and application to catalytic transformation of glycerol to acetol and 1,2-propanediol
- 2014
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Ingår i: Catalysis Today. - : Elsevier BV. - 0920-5861 .- 1873-4308. ; 223, s. 129-137
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Tidskriftsartikel (refereegranskat)abstract
- Different systems consisting of diverse metals (Au, Pt, Pd, Rh) supported on ZnO (5% by weight) were synthesized through the microemulsion technique (ME) and tested for glycerol hydrogenolysis, the main products being hydroxyacetone (acetol) and 1,2-propanediol (1,2-PDO). The solids synthesized using sodium borohydride as the reducing agent (B series) had smaller particle sizes as compared to the use of hydrazine (H series) which, in turn, resulted in a better catalytic performance. This synthetic method allowed us to obtain similar metal particle sizes (2-4 nm) for Pt, Pd and Rh solids in B series, whereas average gold metal particle was higher (> 8 nm) which probably accounts for Au-containing systems being inactive under our experimental conditions. Reactivity order followed the sequence Rh > Pt > Pd. A comparison of the systems synthesized in the present paper through ME technique with those obtained in a previous work through the deposition-precipitation process revealed a higher activity and selectivity to acetol for the former solids which could be related to the presence of surfactant. Moreover, results suggested that metal sites could participate not only in hydrogenation of acetol to 1,2-propanediol but also in the previous dehydration step of glycerol to acetol.
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| 7. |
- Persson, K., et al.
(författare)
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Preparation of alumina-supported palladium catalysts for complete oxidation of methane
- 2003
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Ingår i: Applied Catalysis A. - : Elsevier BV. - 0926-860X .- 1873-3875. ; 249:1, s. 165-174
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Tidskriftsartikel (refereegranskat)abstract
- Alumina-supported palladium catalysts (Pd/Al2O3) have been prepared by incipient wetness (IW), grafting (G) and microemulsion techniques (ME). Two slightly different microemulsion methods have been used (ME1) and (ME2). The catalysts have been calcined at 1000 degreesC for 4 h. The catalysts have then been examined with respect to their activity for the combustion of methane, during heating and cooling ramps. Pd/Al2O3-IW and Pd/Al2O3-ME2 exhibit the highest activity whereas Pd/Al2O3-ME1 and Pd/Al2O3-G are the less active. There is not much of a difference in activity between Pd/Al2O3-IW and Pd/Al2O3-ME2, despite strong differences in palladium particle size observed by TEM analysis. TPO experiments show that Pd/Al2O3-IW and Pd/Al2O3-ME2 present a higher PdO/Pd ratio than the other samples exhibit. The preparation technique affects the reoxidation ability of palladium during cooling, hence the combustion activity of the fresh catalyst, as PdO is more active than Pd for the complete oxidation of methane under lean conditions.
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| 8. |
- Rojas, S., et al.
(författare)
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Preparation of carbon supported Pt and PtRu nanoparticles from microemulsion - Electrocatalysts for fuel cell applications
- 2005
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Ingår i: Applied Catalysis A. - : Elsevier BV. - 0926-860X .- 1873-3875. ; 285:02-jan, s. 24-35
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Tidskriftsartikel (refereegranskat)abstract
- A series of platinum and platinum ruthenium carbon supported electrocatalyst have been prepared by the microemulsion technique. The influence of parameters such as the preparation route, the metal loading and the PtRu stoichiometry on the morphology of the final nanoparticles has been studied. Irrespective the total metal loading, nanosized particles, displaying a narrow size distribution were obtained. In addition, particle size was found to be independent of the metal loading. Structural characteristics of these systems have been studied by XPS, X-ray diffraction, TEM, and TPR-TPO and their textural parameters by N-2 adsorption. The catalytic performance of the samples was evaluated in the electrochemical oxidation of methanol. The influence of the morphology on the catalytic performance of the catalysts is discussed in terms of their synthesis route. © 2005 Elsevier B.V. All rights reserved.
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| 9. |
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| 10. |
- Thevenin, P. O., et al.
(författare)
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Catalytic combustion of methane over cerium-doped palladium catalysts
- 2003
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Ingår i: Journal of Catalysis. - : Elsevier BV. - 0021-9517 .- 1090-2694. ; 215:1, s. 78-86
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Tidskriftsartikel (refereegranskat)abstract
- Various Pd-supported catalysts have been prepared using three different types of alumina as support material: (a) gamma-alumina, (b) Ba-stabilized alumina, and
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