| 1. |
- Jagadeesan, Kishore Kumar, et al.
(författare)
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MALDIViz : A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis
- 2017
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Ingår i: SLAS Discovery. - : Elsevier BV. - 2472-5552. ; 22:10, s. 1246-1252
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Tidskriftsartikel (refereegranskat)abstract
- Recently, mass spectrometry (MS) has emerged as an important tool for high-throughput screening (HTS) providing a direct and label-free detection method, complementing traditional fluorescent and colorimetric methodologies. Among the various MS techniques used for HTS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides many of the characteristics required for high-throughput analyses, such as low cost, speed, and automation. However, visualization and analysis of the large datasets generated by HTS MALDI-MS can pose significant challenges, especially for multiparametric experiments. The datasets can be generated fast, and the complexity of the experimental data (e.g., screening many different sorbent phases, the sorbent mass, and the load, wash, and elution conditions) makes manual data analysis difficult. To address these challenges, a comprehensive informatics tool called MALDIViz was developed. This tool is an R-Shiny-based web application, accessible independently of the operating system and without the need to install any program locally. It has been designed to facilitate easy analysis and visualization of MALDI-MS datasets, comparison of multiplex experiments, and export of the analysis results to high-quality images.
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| 2. |
- Kumar, Saurabh, et al.
(författare)
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Immuno-CoPS (conducting paper strips) for futuristic cost-effective cancer diagnostics
- 2013
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Ingår i: RSC Advances. - : Royal Society of Chemistry (RSC). - 2046-2069. ; 3:29, s. 11846-11853
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Tidskriftsartikel (refereegranskat)abstract
- The fabrication of cost-effective, rapid and specific paper based point-of-care (POC) diagnostics based on the principles of bio-recognition is currently at the vanguard of biomedical research, as it is playing a key role in early diagnosis and treatment, resulting in the inhibition of infection and reducing the mortality rate. In the present report, we demonstrate that biomolecules can be directly attached to a paper matrix covalently, by suitable modification of the paper matrices. A high conductivity is achieved and the porosity can be tailored and has been utilized for the quantitative estimation of bio-analytes. We prove the feasibility of our approach for the detection of cancer biomarkers in a wide detection range.
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| 3. |
- Nogueira Duarte, Mariana, et al.
(författare)
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Solid-phase extraction of the alcohol abuse biomarker phosphatidylethanol using newly synthesized polymeric sorbent materials containing quaternary heterocyclic groups
- 2017
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Ingår i: Journal of Chromatography A. - : Elsevier BV. - 0021-9673. ; 1519, s. 1-8
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Tidskriftsartikel (refereegranskat)abstract
- Phosphatidylethanol (PEth) is an interesting biomarker finding increased use for detecting long term alcohol abuse with high specificity and sensitivity. Prior to detection, sample preparation is an unavoidable step in the work-flow of PEth analysis and new protocols may facilitate it. Solid-phase extraction (SPE) is a versatile sample preparation method widely spread in biomedical laboratories due to its simplicity of use and the possibility of automation. In this work, SPE was used for the first time to directly extract PEth from spiked human plasma and spiked human blood. A library of polymeric SPE materials with different surface functionalities was screened for PEth extraction in order to identify the surface characteristics that control PEth retention and recovery. The plasma samples were diluted 1:10 (v/v) in water and spiked at different concentrations ranging from 0.3 to 5 μM. The library of SPE materials was then evaluated using the proposed SPE method and detection was done by LC–MS/MS. One SPE material efficiently retained and recovered PEth from spiked human plasma. With this insight, four new SPE materials were formulated and synthesized based on the surface characteristics of the best SPE material found in the first screening. These new materials were tested with spiked human blood, to better mimic a real clinical sample. All the newly synthetized materials outperformed the pre-existing commercially available materials. Recovery values for the new SPE materials were found between 29.5% and 48.6% for the extraction of PEth in spiked blood. A material based on quaternized 1-vinylimidazole with a poly(trimethylolpropane trimethacrylate) backbone was found suitable for PEth extraction in spiked blood showing the highest analyte recovery in this experiment, 48.6% ± 6.4%.
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