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Sökning: WFRF:(Jain Rishabh)

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Jain, Rishabh, et al. (författare)
  • Model-Based Design and System on Chip Implementation of DTC and PWM Techniques
  • 2022
  • Ingår i: 2022 IEEE Delhi Section Conference, DELCON 2022. - : Institute of Electrical and Electronics Engineers (IEEE).
  • Konferensbidrag (refereegranskat)abstract
    • This paper presents the implementation procedure of three Pulse Width Modulation (PWM) techniques and a closed-loop motor control method using a model-based design approach. The implemented PWM techniques are the Sinusoidal Pulse Width Modulation (SPWM), Space Vector Pulse Width Modulation (SVPWM), and Selective Harmonic Elimination Pulse Width Modulation (SHEPWM), whereas the closed-loop motor control method is the Direct Torque Control (DTC). These techniques are implemented using a ZedBoard development board which contains a System on a Chip (SoC) Xilinx Zynq-7000. The procedure of implementing these techniques on the ZedBoard and required considerations are discussed. The model-based design approach facilitates rapid implementation without prior knowledge of HDL and ARM programming, which makes it advantageous for students and research work. To verify the integrity of the implemented designs, the ZedBoard is used to control an induction machine (IM), and the results are presented. 
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