| 1. |
- Alm, Kersti, et al.
(författare)
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Cells and holograms : holograms and digital holographic microscopy as a tool to study the morphology of living cells
- 2013
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Ingår i: Holography. - : INTECH. - 9789535111177 ; , s. 335-351
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- We present a method to study the morphology of living, dividing and dying cells using DHM. DHM is a non-invasive, non-destructive and non-phototoxic method which allows the user to perform both qualitative and quantitative measurements of living cells over time. We show here our results on cell division and cell death in single cells. The morphological analyses performed here show changes caused by cell death and cell division, and indicate the possibilities to discriminate between different types of cell death. Cells dying in an apoptosis-like manner display different cell area and cell thickness profiles over time compared to cells dying in a necrosis-like manner, although their volume profiles are very similar. Dividing cells show a characteristic dip in the volume profile, which makes them easily distinguishable. Also, several previous studies show the versatile abilities of DHM. Different cell types have been studied and the morphology has been used to determine cell functionality as well as changes in morphology related to the environment. Cell morphology parameters can be very useful when following the effects of different treatments, the process of differentiation as well as cell growth and cell death. Cell morphology studied by DHM can be useful in toxicology, stem cell and cancer research.
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| 2. |
- Alm, Kersti, et al.
(författare)
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Digital holography and cell studies
- 2011
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Ingår i: Holography, Research and Technologies. - : DKV - Deutscher Kälte- und Klimatechnischer Verein. - 9789533072272 ; , s. 237-252
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Digital holography microscopy (DHM) has developed into a broad field, and one of all the interesting applications is to study cells without staining, labeling or in any other way affecting them. Both fixed and living, dying or dead cells can be studied. The first DHM images showing living cells were published in 2004 and 2005 (Carl et al. 2004, Marquet et al. 2005), making this field of research rather new. Digital holography makes it possible to easily measure cell properties that previously have been very difficult to study, such as cell thickness and volume (Marquet et al. 2005, Mölder et al. 2008). Two of the major advantages of DHM is the 3-D imaging possibility and measurements over time. Digital holography has ben used to study several types of cells, such as nerve cells, red blood cells, stem cells and cancer cells (Emery et al. 2007, Kemper et al. 2006, Langehanenberg et al. 2009) . It has also been applied for studies of cell proliferation, cell movement, sub-cellular structures and cell morphology (Kemper et al. 2009, Yu et al. 2009). Both 2-D and 3-D cell movement can be determined ( Langehanenberg et al. 2009). Even cell viability status can be determined using DHM. Interestingly, it is possible to study both single cells and entire populations simultaneously, allowing for very nuanced studies. Older, well known techniques often require some degree of cell disturbance such as the fluorescent antibody labeling required for fluorescense or confocal microscopy studies. In this paper we will present some of the studies made possible by DHM. We will compare DHM with previously used techniques and discuss the benefits and drawbacks of digital holography cell measurements.
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| 3. |
- El-Schich, Zahra, et al.
(författare)
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Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements : Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry
- 2020
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Ingår i: Applied Sciences. - : MDPI. - 2076-3417. ; 10:14
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Tidskriftsartikel (refereegranskat)abstract
- Breast cancer is the second most common cancer worldwide. Metastasis is the main reason for death in breast cancer, and today, there is a lack of methods to detect and isolate circulating tumor cells (CTCs), mainly due to their heterogeneity and rarity. There are some systems that are designed to detect rare epithelial cancer cells in whole blood based on the most common marker used today, the epithelial cell adhesion molecule (EpCAM). It has been shown that aggressive breast cancer metastases are of non-epithelial origin and are therefore not always detected using EpCAM as a marker. In the present study, we used an in vitro-based circulating tumor cell model comprising a collection of six breast cancer cell lines and white blood cell lines. We used digital holographic cytometry (DHC) to characterize and distinguish between the different cell types by area, volume and thickness. Here, we present significant differences in cell size-related parameters observed when comparing white blood cells and breast cancer cells by using DHC. In conclusion, DHC can be a powerful diagnostic tool for the characterization of CTCs in the blood.
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| 4. |
- El-Schich, Zahra, et al.
(författare)
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Holography : The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics
- 2017
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Ingår i: Holographic Materials and Optical Systems. - : INTECH. - 9789535130383 ; , s. 319-333
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Digital holographic (DH) microscopy is a digital high-resolution holographic imaging technique with the capacity of quantification of cellular conditions without any staining or labeling of cells. The unique measurable parameters are the cell number, cell area, thickness, and volume, which can be coupled to proliferation, migration, cell cycle analysis, viability, and cell death. The technique is cell friendly, fast and simple to use and has unique imaging capabilities for time-lapse investigations on both the single cell and the cell-population levels. The interest for analyzing specifically cell volume changes with DH microscopy, resulting from cytotoxic treatments, drug response, or apoptosis events has recently increased in popularity. We and others have used DH microscopy showing that the technique has the sensitivity to distinguish between different cells and treatments. Recently, DH microscopy has been used for cellular diagnosis in the clinic, providing support for using the concept of DH, e.g., screening of malaria infection of red blood cells (RBC), cervix cancer screening, and sperm quality. Because of its quick and label-free sample handling, DH microscopy will be an important tool in the future for personalized medicine investigations, determining the optimal therapeutic concentration for both different cancer types and individual treatments.
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| 5. |
- Freiburghaus, Catja, et al.
(författare)
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Lactoferricin treatment decreases the rate of cell proliferation of a human colon cancer cell line.
- 2009
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Ingår i: Journal of Dairy Science. - : American Dairy Science Association. - 1525-3198 .- 0022-0302. ; 92:6, s. 2477-2484
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Tidskriftsartikel (refereegranskat)abstract
- Food components modify the risk of cancer at a large number of sites but the mechanism of action is unknown. In the present investigation, we studied the effect of the peptide lactoferricin derived from bovine milk lactoferrin on human colon cancer CaCo-2 cells. The cells were either untreated or treated with 2.0, 0.2, or 0.02 microM lactoferricin. Cell cycle kinetics were investigated with a bromodeoxyuridine DNA flow cytometric method. The results show that lactoferricin treatment slightly but significantly prolonged the S phase of the cell cycle. Lactoferricin treatment lowered the level of cyclin E1, a protein involved in the regulation of genes required for G(1)/S transition and consequently for efficient S phase progression. The slight prolongation of the S phase resulted in a reduction of cell proliferation, which became more apparent after a long treatment time.
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| 6. |
- Janicke, Birgit
(författare)
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Bioactive Food Compounds with Cancer Preventing Effects in the Colon Focus on Ferulic Acid, para-Coumaric Acid and Lactoferricin
- 2009
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Colorectal cancers are globally ranked as the third most common cancer form in terms of incidence and mortality. Diet has long been considered as one of the main factors affecting population outcome regarding development of cancer in the colon. Numerous epidemiological studies have found links between consumption of dietary fibres and milk and reduced risk of cancers including colon cancer. To be able to understand the full extent of the impact of certain foods and diets on public health, it is important to focus on mechanistic effects exerted by single food compounds. This study concerns the effects of the three food compounds, ferulic acid (FA), para-coumaric acid (p-CA) and lactoferricin on colon. The dietary fibres from cereals, fruits and vegetables are rich sources of the phenolic compounds FA and p-CA. Lactoferricin is a peptide derived from the milk protein lactoferrin. The overall objective of this project was to develop an extended knowledge about the mechanisms of the action of FA, p-CA and lactoferricin in the colon with specific emphasis on colon cancer cells. To that end, the study was performed with the colon cancer-derived cell line Caco-2. The main emphasis was on processes related to cell proliferation, as alterations in cell proliferation are essential in cancer prevention and cancer therapy. The Caco-2 cells were treated with physiologically relevant concentrations of FA, p-CA or lactoferricin based on food content of the compounds and consumption. The main methods used were MTT and AlamarBlue assays for analysis of metabolic activity, flow cytometry for analysis of cell cycle phase distribution and cell cycle kinetics, microarray assay for analysis of global gene expression and Western blot for protein detection. The results showed that all three compounds reduced cell proliferation of Caco-2 cells caused by increased cell cycle duration. Specifically, FA treatment induced a delay in the S phase and affected genes that regulate centrosome assembly and the S phase DNA damage checkpoints such as CEP2, CETN3 and RABGAP1. p-CA treatment induced a delay in the G2/M phase and affected genes in the cell cycle regulating system including MYC, CDKN1A, PCNA, CDC25A, ODC1, CCNA2 and CCNB1. Lactoferricin treatment induced a delay in the S phase and affected cyclin E1 protein expression. A daily dose of one or more compounds over a long time span, years and decades, that decreases the number of total cell divisions substantially, may reduce the overall risk of cancer development. This is probably the most important message in this study to be carried along for the future.
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| 7. |
- Janicke, Birgit, et al.
(författare)
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Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2
- 2005
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Ingår i: Journal of Agricultural and Food Chemistry. - : American Chemical Society (ACS). - 0021-8561 .- 1520-5118. ; 53:17, s. 6658-6665
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Tidskriftsartikel (refereegranskat)abstract
- Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer.
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| 8. |
- Janicke, Birgit, et al.
(författare)
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The antiproliferative effect of dietary fibre phenolic compounds ferulic acid and p-coumaric acid on the cell cycle in Caco-2 cells
- 2011
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Ingår i: Nutrition and Cancer. - : Informa UK Limited. - 1532-7914 .- 0163-5581. ; 63, s. 611-622
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Tidskriftsartikel (refereegranskat)abstract
- Epidemiological and animal studies have shown that dietary fiber is protective against the development of colon cancer. Dietary fiber is a rich source of the hydroxycinnamic acids ferulic acid (FA) and p-coumaric acid (p-CA), which both may contribute to the protective effect. We have investigated the effects of FA and p-CA treatment on global gene expression in Caco-2 colon cancer cells. The Caco-2 cells were treated with 150 μM FA or p-CA for 24 h, and gene expression was analyzed with cDNA microarray technique. A total of 517 genes were significantly affected by FA and 901 by p-CA. As we previously have found that FA or p-CA treatment delayed cell cycle progression, we focused on genes involved in proliferation and cell cycle regulation. The expressions of a number of genes involved in centrosome assembly, such as RABGAP1 and CEP2, were upregulated by FA treatment as well as the gene for the S phase checkpoint protein SMC1L1. p-CA treatment upregulated CDKN1A expression and downregulated CCNA2, CCNB1, MYC, and ODC1. Some proteins corresponding to the affected genes were also studied. Taken together, the changes found can partly explain the effects of FA or p-CA treatment on cell cycle progression, specifically in the S phase by FA and G(2)/M phase by p-CA treatment.
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| 9. |
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| 10. |
- Kamlund, Sofia, et al.
(författare)
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Influence of salinomycin treatment on division and movement of individual cancer cells cultured in normoxia or hypoxia evaluated with time-lapse digital holographic microscopy
- 2017
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Ingår i: Cell Cycle. - : Informa UK Limited. - 1538-4101 .- 1551-4005. ; 16:21, s. 2128-2138
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Tidskriftsartikel (refereegranskat)abstract
- Most studies on new cancer drugs are based on population-derived data, where the absence of response of a small population may pass unnoticed. Thus, individual longitudinal tracking of cells is important for the future development of efficient cancer treatments. We have used digital holographic microscopy to track individual JIMT-1 human breast cancer cells and L929 mouse fibroblast cultivated in normoxia or hypoxia. In addition, JIMT-1 cells were treated with salinomycin, a cancer stem cell targeting compound. Three-day time-lapse movies were captured and individual cells were analysed with respect to cell division (cell cycle length) and cell movement. Comparing population-doubling time derived from population-based growth curves and individual cell cycle time data from time-lapse movies show that the former hide a sub-population of dividing cells. Salinomycin treatment increased the motility of cells, however, this motility did not result in an increased distant migration i.e. the cells increased their local movement. MCF-7 breast cancer cells showed similar motility behaviour as salinomycin-treated JIMT-1 cells. We suggest that combining features, such as motility and migration, can be used to distinguish cancer cells with mesenchymal (JIMT-1) and epithelial (MCF-7) features. The data clearly emphasize the importance of longitudinal cell tracking to understand the biology of individual cells under different conditions.
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