1. |
- Wang, Jijing, et al.
(författare)
-
First Immunoassay for Measuring Isoaspartate in Human Serum Albumin
- 2021
-
Ingår i: Molecules. - : MDPI. - 1431-5157 .- 1420-3049. ; 26:21
-
Tidskriftsartikel (refereegranskat)abstract
- Isoaspartate (isoAsp) is a damaging amino acid residue formed in proteins mostly as a result of spontaneous deamidation of asparaginyl residues. An association has been found between isoAsp in human serum albumin (HSA) and Alzheimer’s disease (AD). Here we report on a novel monoclonal antibody (mAb) 1A3 with excellent specificity to isoAsp in the functionally important domain of HSA. Based on 1A3 mAb, an indirect enzyme-linked immunosorbent assay (ELISA) was developed, and the isoAsp occupancy in 100 healthy plasma samples was quantified for the first time, providing the average value of (0.74 ± 0.13)%. These results suggest potential of isoAsp measurements for supplementary AD diagnostics as well as for assessing the freshness of stored donor blood and its suitability for transfusion.
|
|