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- Bauren, G, et al.
(författare)
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Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei
- 1996
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Ingår i: The Journal of cell biology. - : Rockefeller University Press. - 0021-9525 .- 1540-8140. ; 133:5, s. 929-941
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Tidskriftsartikel (refereegranskat)abstract
- We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. The snRNPs and an SR non-snRNP splicing factor are present in excess, mainly distributed throughout the interchromatin. Approximately 10% of the U2 snRNP and an SR non-snRNP splicing factor are associated with the chromosomes, highly enriched in active gene loci where they are bound to RNA. We demonstrate that the splicing factors are specifically recruited to a defined gene upon induction of transcription during physiological conditions. Concomitantly, the splicing factors leave gene loci in which transcription is turned off. We also demonstrated that upon general transcription inhibition, the splicing factors redistribute from active gene loci to the interchromatin. Our findings demonstrate the dynamic intranuclear organization of splicing factors and a tight linkage between transcription and the intranuclear organization of the splicing machinery.
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- He, ZC, et al.
(författare)
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Composition of Urine Collected from Non-Stone-Forming Chinese Persons during Different Short-Term Periods of the Day
- 2022
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Ingår i: Urologia internationalis. - : S. Karger AG. - 1423-0399 .- 0042-1138. ; 106:3, s. 227-234
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study in a small group of non-stone-forming Chinese persons was to measure the levels of supersaturation with calcium oxalate and calcium phosphate and pH with the aim of confirming if any of the different short-term urine samples were better for risk evaluation than a 24-h sample. Nine normal men and 1 woman collected urine during 4 periods of the day. <i>Period 1</i> between 08 and 12 h, <i>Period 2</i> between 12 and 18 h, <i>Period 3</i> between 18 and 22 h, and <i>Period 4</i> between 22 and 08 h. Each sample was analysed for calcium, oxalate, citrate, magnesium and phosphate, and estimates of supersaturation with calcium oxalate (CaOx) and calcium phosphate (CaP) were expressed in terms of AP(CaOx) and AP(CaP) index. An estimate of the solute load of CaOx was also calculated. Urine composition for 24-h urine (<i>Period 24</i>) was obtained mathematically from the analysed variables. Urine composition corresponding to 14-h urine portions 22–12 h (<i>Period 14</i>N) and 08–22 h (<i>Period 14 D</i>) were calculated. The lowest pH levels were recorded in <i>Period 1</i> urine. The highest level of AP(CaOx) index was recorded during <i>Period 1</i>, and the product AP(CaOx) index × 10<sup>7</sup> × hydrogen ion concentration was significantly higher in Period 1 urine than in 24-h urine (<i>p</i> = 0.02). Also, the product SL(CaOx) × 10<sup>7</sup> × hydrogen ion concentration was significantly higher in <i>Period 1</i> urine (<i>p</i> = 0.02). Low AP (CaP) index levels were recorded in <i>Period 4</i>, but also in all periods following dietary loads of calcium and phosphate. With the important reservation that the analytical results were obtained from non-stone-forming persons, the conclusion is that analysis of urine samples collected between 08 and 12 h might be an alternative to 24-h urine. The risk evaluation might advantageously be expressed either in terms of the product AP(CaOx) index × 10<sup>7</sup> × hydrogen ion concentration or the product SL(CaOx) × 10<sup>7</sup> × hydrogen ion concentration.
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