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| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Kristan, Matej, et al.
(författare)
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The Ninth Visual Object Tracking VOT2021 Challenge Results
- 2021
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Ingår i: 2021 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW 2021). - : IEEE COMPUTER SOC. - 9781665401913 ; , s. 2711-2738
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2021 is the ninth annual tracker benchmarking activity organized by the VOT initiative. Results of 71 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in recent years. The VOT2021 challenge was composed of four sub-challenges focusing on different tracking domains: (i) VOT-ST2021 challenge focused on short-term tracking in RGB, (ii) VOT-RT2021 challenge focused on "real-time" short-term tracking in RGB, (iii) VOT-LT2021 focused on long-term tracking, namely coping with target disappearance and reappearance and (iv) VOT-RGBD2021 challenge focused on long-term tracking in RGB and depth imagery. The VOT-ST2021 dataset was refreshed, while VOT-RGBD2021 introduces a training dataset and sequestered dataset for winner identification. The source code for most of the trackers, the datasets, the evaluation kit and the results along with the source code for most trackers are publicly available at the challenge website(1).
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| 4. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 5. |
- Li, Xuefeng, et al.
(författare)
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Fully aromatic ionomers with precisely sequenced sulfonated moieties for enhanced proton conductivity
- 2012
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Ingår i: Macromolecules. - : American Chemical Society (ACS). - 0024-9297 .- 1520-5835. ; 45:3, s. 1447-1459
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Tidskriftsartikel (refereegranskat)abstract
- Abstract in UndeterminedA series of six fully aromatic ionomers with precisely sequenced sulfonated sites along the polymer chains have been designed, prepared and characterized as proton-exchange membranes. Two straight-forward and efficient synthetic strategies based on Ullmann ether reactions and a Baeyer-Villiger rearrangement were devised to obtain bisphenol monomers with four or six phenylene units linked exclusively by ether bridges to avoid transetherification reactions. Polycondensations of these bisphenol monomers with mono- or disulfonated dihalide monomers gave high molecular weight poly(arylene ether), poly(arylene ether sulfone) and polyarylene ether ketone) homopolymers having microblock-like structures with sulfonated moieties separated by monodisperse non-sulfonated oligo(ether) spacers. The nanoscale morphology and properties of solvent cast membranes were closely related to the nature of the oligo(ether) spacers. Small angle X-ray scattering (SAXS) measurements showed intense scattering and very narrow ionomer peaks with second-order features for the polymers with the six-ring spacers. This clearly indicated that the controlled ionic sequencing enabled self-assembly of ionic aggregates with a much higher degree of organization in relation to a corresponding aromatic ionomer with a statistical distribution of the sulfonate groups. At an identical acid content, the ionomers containing meta ether linkages had lower glass transition temperatures than the all-para materials, leading to a higher water uptake and proton conductivity of the former ionomers. A membrane with an ion exchange capacity (IEC) of 2.05 meq g-1 and containing exclusively para linkages reached the same level of proton conductivity as Nafion® at 100% relative humidity (RH), and also had an excellent dimensional stability in boiling water. Under reduced RH, the conductivity of this membrane greatly exceeded that of a membrane based on a statistical copolymer analogue with a similar ionic content.
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| 6. |
- Liu, Tang hong, et al.
(författare)
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Differences in aerodynamic effects when trains with different marshalling forms and lengths enter a tunnel
- 2019
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Ingår i: Tunnelling and Underground Space Technology. - : Elsevier BV. - 0886-7798. ; 84, s. 70-81
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Tidskriftsartikel (refereegranskat)abstract
- Unsteady Reynolds-averaged Navier-Stokes (URANS) simulations were performed to simulate trains with different marshalling forms and lengths entering a tunnel. Three models, including a short train, a double train and a long train, were used to analyse the influence of the train configuration on the pressure variations during a train's passage. The results of the numerical predictions were validated against existing experimental data, with which they showed good agreement. The differences in the maximum pressure peak distribution and the pressure fluctuations were analysed by means of Mach diagrams. The results show that the grouping length exerts a considerable influence on the amplitude of the pressure on the train body and that the influence of the grouping length on the pressure variation on the tunnel wall varies with the location in the tunnel. The tunnel space can be divided into three and four zones with regard to the influences on the maximum positive and negative pressure values, respectively. The different marshalling forms also influence the maximum peak values and local profiles of the pressure history curves, although this influence is much slighter than that of the train's grouping length.
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| 7. |
- Liu, Tang hong, et al.
(författare)
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Field test measurement of the dynamic tightness performance of high-speed trains and study on its influencing factors
- 2019
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Ingår i: Measurement: Journal of the International Measurement Confederation. - : Elsevier BV. - 0263-2241. ; 138, s. 602-613
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Tidskriftsartikel (refereegranskat)abstract
- During the actual operation of a high-speed train, the airtight performance of the carriages will change significantly. Based on a series of field tests, the external and interior pressure variations of train carriages are measured and then the dynamic pressure tightness coefficient are analysed quantitatively for investigating the airtight performance of a train passing at various speeds through tunnels of different lengths. It is found that the amplitude of the external pressure variations is proportional to the square of the train speed, while the amplitude inside the vehicle is approximately proportional to the speed to the 0.7th power due to the airtight effect. The dynamic pressure tightness coefficient increases with increasing tunnel length and decreases with increasing train speed. Regarding the effect of the carriage location, the coefficient shows an overall increasing trend from the train head to tail.
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| 8. |
- Liu, Tanghong, 1976, et al.
(författare)
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Wave effects in a realistic tunnel induced by the passage of high-speed trains
- 2019
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Ingår i: Tunnelling and Underground Space Technology. - : Elsevier BV. - 0886-7798. ; 86, s. 224-235
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Tidskriftsartikel (refereegranskat)abstract
- As a high-speed train passes through a tunnel, aerodynamic pressure waves propagate backwards and forward in the tunnel, and they persist for a long time after the train is gone. Understanding the aerodynamic characteristics influenced by various factors on a tunnel is essential for ensuring the safety of tunnel structures. Field measurements were conducted in a 2812 m-long tunnel to systematically investigate the pressure characteristics during the passage of CRH2-150C and CRH380AL high-speed trains through the tunnel, both in single-train and intersecting cases. The results reveal that as the train speed increases, the location of the maximum peak-to-peak pressure variation shifts toward the tunnel entrance, mainly driven by the change in the negative pressure peak. The train length induces significant differences in peak pressures on the tunnel wall in the middle of the tunnel, and a long train brings more massive subsequent pressure waves than a short train in the post-train stage, but they decay faster. The intersection of two trains in the tunnel not only causes a significant change in the peak pressure and its location, but also has a significant effect on damage factor (the damage level on a structure subjected to a specific load) after train leaving the tunnel, with a 65.3% of this factor during trains operating in the tunnel.
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| 9. |
- Xia, Yutao, 1995, et al.
(författare)
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Aerodynamic influences of typical windbreak wall types on a high-speed train under crosswinds
- 2022
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Ingår i: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 231
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Tidskriftsartikel (refereegranskat)abstract
- The effect of windbreak wall types on their windproof effects on trains has been systematically investigated by comparing the flow field around a high-speed train behind three typical windbreak walls. These three typical windbreak walls are widely used along the windy railway lines in China, which include the earth embankment–type windbreak wall (EW), the road cutting–type windbreak wall (RW), and the straight reinforced concrete–type windbreak wall (SW). We compared the time-averaged crosswind-induced flows and aerodynamic performances of high-speed trains behind these three windbreak walls with the same height using numerical simulations. The results revealed that the windbreak wall geometric shape effects on the aerodynamic load coefficients varied according to the type of aerodynamic load and carriage marshalling positions but barely varied with yaw angles. The total drag coefficients of the train in the EW and RW were approximately 50–60% of that in the SW under the two smaller yaw angles and were only 30–40% for the largest yaw angle. For the absolute value of the side force and rolling moments coefficients, the maximum values for the head and middle cars both appeared in the RW, and the corresponding minimum values were obtained in the SW. The maximum and minimum values for the tail car were obtained at the SW and EW, respectively. The maximum of the rolling moment coefficient among three carriages in the SW was approximately 45–60% of that in the EW and only 30–40% of that in the RW. The time-averaged train surface pressure coefficient and flow patterns were similar between the EW and RW, which showed apparent differences from those in the SW. The SW provided a strong blocking effect on the incoming windward airflow and avoided the direct impact on the train. Compared with the RW, the uplifting effect of the EW's windward slope on the incoming flow further reduced the crosswind effect on trains. In addition, the dominating frequency characteristics of the aerodynamic loads were significantly affected by windbreak wall types. These findings provide a systematic understanding of the time-averaged aerodynamics of trains behind these three typical windbreak walls.
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| 10. |
- Zhao, Cheng-Shou, et al.
(författare)
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Thermal decomposition behaviors of a self-intumescent flame retardant epoxy resin
- 2022
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Ingår i: Journal of Applied Polymer Science. - : John Wiley & Sons. - 0021-8995 .- 1097-4628. ; 139:18
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Tidskriftsartikel (refereegranskat)abstract
- This paper focuses on revealing the thermal decomposition behaviors of a self-intumescent flame retardant (IFR) epoxy (EP) resin (EP/15%APP-Cu2O) employing 12 wt% ammonium polyphosphate (APP) as a halogen-free flame retardant and 3 wt% copper (I) oxide (Cu2O) as char forming rate regulator. Initially, the thermal stability of EP/15%APP-Cu2O was analyzed and compared to virgin EP resin and flame retardant EP (EP/15%APP) containing 15 wt% APP as flame retardant by thermogravimetric analysis test at different heating rates under nitrogen atmosphere. It was shown that the incorporation of APP altered the decomposition pathway of EP and decreased the onset decomposition temperature. Luckily, compared to EP/15%APP, the onset decomposition temperature of EP/15%APP-Cu2O was just slightly reduced from 300.4 to 292.8 degrees C. Then, the thermal degradation kinetics of EP, EP/15%APP and EP/15%APP-Cu2O were further evaluated by Kissinger and Flynn-Wall-Ozawa methods. It was worth noted that the addition of APP or APP-Cu2O enhanced the thermal degradation activation energies of EP, which contributed to the protective effect of the char formation. Particularly, the incorporation of 3 wt% Cu2O significantly decreased the thermal degradation activation energies at the early decomposition stage of EP. This may be the main contribution for intumescent char formation, which resulted in higher fire safety of EP/15%APP-Cu2O compared to EP/15%APP. These information can potentially help to develop alternative IFR systems.
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