| 1. |
- Johannsen, Annsofi, et al.
(författare)
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Dental implants from the patients perspective : transition from tooth loss, through amputation to implants – negative and positive trajectories
- 2012
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Ingår i: Journal of Clinical Periodontology. - 0303-6979 .- 1600-051X. ; 39:7, s. 681-687
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Tidskriftsartikel (refereegranskat)abstract
- Aim The aim of this study was to explore patients' expectations on and experiences from dental implant treatment through deep-interview technique. Material & Methods A qualitative study design was chosen and 17 patients were interviewed by open-ended questions. All patients in the study had a previous history of periodontal disease with, in most cases, many years of treatment. The interviews were transcribed; a coding process was used according to qualitative conventional content analysis. Results In the analysis, a core category was identified as “Transition from tooth loss, to ‘Amputation’, and to implants – negative and positive trajectories”. When the patients faced the fact that it was not possible to keep the teeth any longer, a period of fear, shame and denial, which also affected their social life negatively followed. After they received their implants and the chewing ability and appearance became better, it also improved their quality of life. Conclusion Treatment with dental implants improved function, enhanced self-esteem, social life and, thus quality of life. In clinical practice, information about dental implants and motivational strategies are needed during the period before getting dental implants. Follow-up is important thereafter, capturing both the pros and cons with implants.
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| 2. |
- Johannsen, Annsofi, et al.
(författare)
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Dental implants from the patients perspective : transition from tooth loss, through amputation to implants – negative and positive trajectories
- 2012
-
Ingår i: Journal of Clinical Periodontology. - : Blackwell Munksgaard. - 0303-6979 .- 1600-051X. ; 39:7, s. 681-687
-
Tidskriftsartikel (refereegranskat)abstract
- Aim The aim of this study was to explore patients' expectations on and experiences from dental implant treatment through deep-interview technique. Material & Methods A qualitative study design was chosen and 17 patients were interviewed by open-ended questions. All patients in the study had a previous history of periodontal disease with, in most cases, many years of treatment. The interviews were transcribed; a coding process was used according to qualitative conventional content analysis. Results In the analysis, a core category was identified as “Transition from tooth loss, to ‘Amputation’, and to implants – negative and positive trajectories”. When the patients faced the fact that it was not possible to keep the teeth any longer, a period of fear, shame and denial, which also affected their social life negatively followed. After they received their implants and the chewing ability and appearance became better, it also improved their quality of life. Conclusion Treatment with dental implants improved function, enhanced self-esteem, social life and, thus quality of life. In clinical practice, information about dental implants and motivational strategies are needed during the period before getting dental implants. Follow-up is important thereafter, capturing both the pros and cons with implants.
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| 3. |
- Johannsen, Gunnar, et al.
(författare)
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The importance of measuring toothpaste abrasivity in both a quantitative and qualitative way
- 2013
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Ingår i: Acta Odontologica Scandinavica. - : Informa UK Limited. - 0001-6357 .- 1502-3850. ; 71:3-4, s. 508-517
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Tidskriftsartikel (refereegranskat)abstract
- Objective. To evaluate the relative abrasivity of different toothpastes and polishing pastes both qualitatively and quantitatively. Materials and methods. Acrylic plates were exposed to brushing in a brushing machine with a toothpaste/water slurry for 1 and 6 h. Twelve different toothpastes were used and also four different polishing pastes. The results were evaluated using a profilometer after 1 and 6 h of brushing (corresponding to 2000 and 12 000 double strokes, respectively). A surface roughness value (Ra-value) and also a volume loss value were calculated from the profilometer measurements. These values were then correlated to each other. An unpaired t-test for the difference in the abrasion values between the toothpastes and the abrasion values over time was used. Results. The polishing paste RDA (R) 170 yielded higher Ra-values than RDA 250 (R), both after 1 and 6 h of brushing (1.01 +/- 0.22 and 8.99 +/- 1.55 compared to 0.63 +/- 0.26 and 7.83 +/- 5.89, respectively) as well as volume loss values (3.71 +/- 0.17 and 20.20 +/- 2.41 compared to 2.15 +/- 1.41 and 14.79 +/- 11.76, respectively), thus poor correlations between the RDA and Ra and Volume loss values were shown. Among the toothpastes, Apotekets (R) showed the highest Ra value after 1 h of brushing and Pepsodent (R) whitening after 6 h of brushing. Pepsodent (R) whitening also showed the highest volume loss values, both after 1 and 6 h of brushing. Conclusion. This study emphasizes the importance of not only considering the RDA value, but also a roughness value, when describing the abrasivity of a toothpaste. Furthermore, it can be concluded that so called 'whitening' toothpastes do not necessarily have a higher abrasive effect than other toothpastes.
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| 4. |
- Lira-Junior, Ronaldo, et al.
(författare)
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S100A12 Expression Is Modulated During Monocyte Differentiation and Reflects Periodontitis Severity
- 2020
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Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- S100A12 is a calcium-binding protein of the S100 subfamily of myeloid-related proteins that acts as an alarmin to induce a pro-inflammatory innate immune response. It has been linked to several chronic inflammatory diseases, however its role in the common oral immunopathology periodontitis is largely unknown. Previous in vitro monoculture experiments indicate that S100A12 production decreases during monocyte differentiation stages, while the regulation within tissue is poorly defined. This study evaluated S100A12 expression in monocyte subsets, during monocyte-to-macrophage differentiation and following polarization, both in monoculture and in a tissue context, utilizing a three-dimensional co-culture oral tissue model. Further, we explored the involvement of S100A12 in periodontitis by analyzing its expression in peripheral circulation and gingival tissue, as well as in saliva. We found that S100A12 expression was higher in classical than in non-classical monocytes. S100A12 expression and protein secretion declined significantly during monocyte-to-macrophage differentiation, while polarization of monocyte-derived macrophages had no effect on either. Peripheral monocytes from periodontitis patients had higher S100A12 expression than monocytes from controls, a difference particularly observed in the intermediate and non-classical monocyte subsets. Further, monocytes from periodontitis patients displayed an increased secretion of S100A12 compared with monocytes from controls. In oral tissue cultures, monocyte differentiation resulted in increased S100A12 secretion over time, which further increased after inflammatory stimuli. Likewise, S100A12 expression was higher in gingival tissue from periodontitis patients where monocyte-derived cells exhibited higher expression of S100A12 in comparison to non-periodontitis tissue. In line with our findings, patients with severe periodontitis had significantly higher levels of S100A12 in saliva compared to non-periodontitis patients, and the levels correlated to clinical periodontal parameters. Taken together, S100A12 is predominantly secreted by monocytes rather than by monocyte-derived cells. Moreover, S100A12 is increased in inflamed tissue cultures, potentially as a result of enhanced production by monocyte-derived cells. This study implicates the involvement of S100A12 in periodontitis pathogenesis, as evidenced by increased S100A12 expression in inflamed gingival tissue, which may be due to altered circulatory monocytes in periodontitis.
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| 5. |
- Lundmark, Anna, et al.
(författare)
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Identification of Salivary Microbiota and Its Association With Host Inflammatory Mediators in Periodontitis
- 2019
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Ingår i: Frontiers in Cellular and Infection Microbiology. - : Frontiers Media S.A.. - 2235-2988. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Periodontitis is a microbial-induced chronic inflammatory disease, which may not only result in tooth loss, but can also contribute to the development of various systemic diseases. The transition from healthy to diseased periodontium depends on microbial dysbiosis and impaired host immune response. Although periodontitis is a common disease as well as associated with various systemic inflammatory conditions, the taxonomic profiling of the salivary microbiota in periodontitis and its association with host immune and inflammatory mediators has not been reported. Therefore, the aim of this study was to identify key pathogens and their potential interaction with the host's inflammatory mediators in saliva samples for periodontitis risk assessment. The microbial 16S rRNA gene sequencing and the levels of inflammatory mediators were performed in saliva samples from patients with chronic periodontitis and periodontally healthy control subjects. The salivary microbial community composition differed significantly between patients with chronic periodontitis and healthy controls. Our analyses identified a number of microbes, including bacteria assigned to Eubacterium saphenum, Tannerella forsythia, Filifactor alocis, Streptococcus mitis/parasanguinis, Parvimonas micra, Prevotella sp., Phocaeicola sp., and Fretibacterium sp. as more abundant in periodontitis, compared to healthy controls. In samples from healthy individuals, we identified Campylobacter concisus, and Veillonella sp. as more abundant. Integrative analysis of the microbiota and inflammatory mediators/cytokines revealed associations that included positive correlations between the pathogens Treponema sp. and Selenomas sp. and the cytokines chitinase 3-like 1, sIL-6R alpha, sTNF-R1, and gp 130/sIL-6R beta. In addition, a negative correlation was identified between IL-10 and Filifactor alocis. Our results reveal distinct and disease-specific patterns of salivary microbial composition between patients with periodontitis and healthy controls, as well as significant correlations between microbiota and host-mediated inflammatory cytokines. The positive correlations between the pathogens Treponema sp. and Selenomas sp. and the cytokines chitinase 3-like 1, sIL-6R alpha, sTNF-R1, and gp 130/sIL-6R beta might have the future potential to serve as a combined bacteria-host salivary biomarker panel for diagnosis of the chronic infectious disease periodontitis. However, further studies are required to determine the capacity of these microbes and inflammatory mediators as a salivary biomarker panel for periodontitis.
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| 6. |
- Lundmark, Anna, et al.
(författare)
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Mucin 4 and matrix metalloproteinase 7 as novel salivary biomarkers for periodontitis
- 2017
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Ingår i: Journal of Clinical Periodontology. - : John Wiley & Sons. - 0303-6979 .- 1600-051X. ; 44:3, s. 247-254
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Tidskriftsartikel (refereegranskat)abstract
- Aim: Periodontitis is a chronic inflammatory disease, characterized by irreversible destruction of tooth-supporting tissue including alveolar bone. We recently reported mucin 4 ( MUC4) and matrix metalloproteinase 7 (MMP7) as highly associated with periodontitis in gingival tissue biopsies. The aim of this study was to further investigate the levels of MUC4 and MMP7 in saliva and gingival crevicular fluid (GCF) samples of patients with periodontitis. Materials and Methods: Saliva and GCF samples were collected from periodontitis patients and healthy controls. The levels of MUC4, MMP7, and total protein concentrations were analysed using ELISA or Bradford assay. Results: MUC4 levels were significantly lower in saliva and GCF from periodontitis patients relative to healthy controls. MMP7 levels were significantly higher in saliva and GCF from periodontitis patients. Multivariate analysis revealed that MUC4 was significantly associated with periodontitis after adjusting for age and smoking habits and, moreover, that the combination of MUC4 and MMP7 accurately discriminated periodontitis from healthy controls. Conclusions: MUC4 and MMP7 may be utilized as possible novel biomarkers for periodontitis.
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| 7. |
- Lundmark, Anna, et al.
(författare)
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Transcriptome analysis reveals mucin 4 to be highly associated with periodontitis and identifies pleckstrin as a link to systemic diseases
- 2015
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Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 5
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Tidskriftsartikel (refereegranskat)abstract
- The multifactorial chronic inflammatory disease periodontitis, which is characterized by destruction of tooth-supporting tissues, has also been implicated as a risk factor for various systemic diseases. Although periodontitis has been studied extensively, neither disease-specific biomarkers nor therapeutic targets have been identified, nor its link with systemic diseases. Here, we analyzed the global transcriptome of periodontitis and compared its gene expression profile with those of other inflammatory conditions, including cardiovascular disease (CVD), rheumatoid arthritis (RA), and ulcerative colitis (UC). Gingival biopsies from 62 patients with periodontitis and 62 healthy subjects were subjected to RNA sequencing. The up-regulated genes in periodontitis were related to inflammation, wounding and defense response, and apoptosis, whereas down-regulated genes were related to extracellular matrix organization and structural support. The most highly up-regulated gene was mucin 4 (MUC4), and its protein product was confirmed to be over-expressed in periodontitis. When comparing the expression profile of periodontitis with other inflammatory diseases, several gene ontology categories, including inflammatory response, cell death, cell motion, and homeostatic processes, were identified as common to all diseases. Only one gene, pleckstrin (PLEK), was significantly overexpressed in periodontitis, CVD, RA, and UC, implicating this gene as an important networking link between these chronic inflammatory diseases.
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| 8. |
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| 9. |
- Sherina, Natalia, et al.
(författare)
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Antibodies to a Citrullinated Porphyromonas gingivalis Epitope Are Increased in Early Rheumatoid Arthritis, and Can Be Produced by Gingival Tissue B Cells : Implications for a Bacterial Origin in RA Etiology
- 2022
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Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 13
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Tidskriftsartikel (refereegranskat)abstract
- Based on the epidemiological link between periodontitis and rheumatoid arthritis (RA), and the unique feature of the periodontal bacterium Porphyromonas gingivalis to citrullinate proteins, it has been suggested that production of anti-citrullinated protein antibodies (ACPA), which are present in a majority of RA patients, may be triggered in the gum mucosa. To address this hypothesis, we investigated the antibody response to a citrullinated P. gingivalis peptide in relation to the autoimmune ACPA response in early RA, and examined citrulline-reactivity in monoclonal antibodies derived from human gingival B cells. Antibodies to a citrullinated peptide derived from P. gingivalis (denoted CPP3) and human citrullinated peptides were analyzed by multiplex array in 2,807 RA patients and 372 controls; associations with RA risk factors and clinical features were examined. B cells from inflamed gingival tissue were single-cell sorted, and immunoglobulin (Ig) genes were amplified, sequenced, cloned and expressed (n=63) as recombinant monoclonal antibodies, and assayed for citrulline-reactivities by enzyme-linked immunosorbent assay. Additionally, affinity-purified polyclonal anti-cyclic-citrullinated peptide (CCP2) IgG, and monoclonal antibodies derived from RA blood and synovial fluid B cells (n=175), were screened for CPP3-reactivity. Elevated anti-CPP3 antibody levels were detected in RA (11%), mainly CCP2+ RA, compared to controls (2%), p<0.0001, with a significant association to HLA-DRB1 shared epitope alleles, smoking and baseline pain, but with low correlation to autoimmune ACPA fine-specificities. Monoclonal antibodies derived from gingival B cells showed cross-reactivity between P. gingivalis CPP3 and human citrullinated peptides, and a CPP3+/CCP2+ clone, derived from an RA blood memory B cell, was identified. Our data support the possibility that immunity to P. gingivalis derived citrullinated antigens, triggered in the inflamed gum mucosa, may contribute to the presence of ACPA in RA patients, through mechanisms of molecular mimicry.
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