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- Högström, Ulf, et al.
(författare)
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Momentum fluxes and wind gradients in the marine boundary layer : a multi platform study
- 2008
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Ingår i: Boreal environment research. - 1239-6095 .- 1797-2469. ; 13:6, s. 475-502
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Tidskriftsartikel (refereegranskat)abstract
- During five autumn weeks, measurements of turbulent fluxes were obtained in the Baltic Sea at three levels on a 30-m tower and two levels on an ASIS buoy 4 km from the tower together with profiles of wind and temperature. Wave data and SST were obtained from ASIS. In the mean, momentum fluxes measured on the tower and on ASIS during onshore winds agree closely. Dimensionless wind gradients phi(m)(z/L) for (i) stable conditions are linear in z/L (L is the Obukhov length); (ii) unstable, growing sea conditions are much smaller than predicted by 'standard' equations, due to an indirect effect of the boundary layer height. Individual wind profiles extrapolated from ASIS to tower by integration of phi(m)(z/L) deviate by about 0.5 m s(-1) from measured values, but corresponding mean profiles agree well for all levels from 1.18 m to 30 m. This random variation in the wind field is shown to be related to inherent dynamics of the atmospheric surface layer.
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| 3. |
- Aspenström, Pontus, et al.
(författare)
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The diaphanous-related formin DAAM1 collaborates with the Rho GTPases RhoA and Cdc42, CIP4 and Src in regulating cell morphogenesis and actin dynamics
- 2006
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Ingår i: Experimental Cell Research. - : Elsevier BV. - 0014-4827 .- 1090-2422. ; 312:12, s. 2180-2194
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Tidskriftsartikel (refereegranskat)abstract
- Binding partners for the Cdc42 effector CIP4 were identified by the yeast two-hybrid system, as well as by testing potential CIP4-binding proteins in coimmunoprecipitation experiments. One of the CIP4-binding proteins, DAAM1, was characterised in more detail. DAAM1 is a ubiquitously expressed member of the mammalian diaphanous-related formins, which include proteins such as mDia1 and mDia2. DAAM1 was shown to bind to the SH3 domain of CIP4 in vivo. Ectopically expressed DAAM1 localised in dotted pattern at the dorsal side of transfected cells and the protein was accumulated in the proximity to the microtubule organising centre. Moreover, ectopic expression of DAAM1 induced a marked alteration of the cell morphology, seen as rounding up of the cells, the formation of branched protrusions as well as a reduction of stress-fibres in the transfected cells. Coimmunoprecipitation experiments demonstrated that DAAM1 bound to RhoA and Cdc42 in a GTP-dependent manner. Moreover, DAAM1 was found to interact and collaborate with the non-receptor tyrosine kinase Src in the formation of branched protrusions. Taken together, our data indicate that DAAM1 communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system.
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| 5. |
- Englund, Hillevi, 1980-, et al.
(författare)
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Sensitive ELISA detection of amyloid-β protofibrils in biological samples
- 2007
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Ingår i: Journal of Neurochemistry. - : Wiley. - 0022-3042 .- 1471-4159. ; 103:1, s. 334-345
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Tidskriftsartikel (refereegranskat)abstract
- Amyloid-β (Aβ) protofibrils are known intermediates of the in vitro Aβ aggregation process and the protofibrillogenic Arctic mutation (APPE693G) provides clinical support for a pathogenic role of Aβ protofibrils in Alzheimer's disease (AD). To verify their in vivo relevance and to establish a quantitative Aβ protofibril immunoassay, Aβ conformation dependent monoclonal antibodies were generated. One of these antibodies, mAb158 (IgG2a), was used in a sandwich ELISA to specifically detect picomolar concentrations of Aβ protofibrils without interference from Aβ monomers or the amyloid precursor protein (APP). The specificity and biological significance of this ELISA was demonstrated using cell cultures and transgenic mouse models expressing human APP containing the Swedish mutation (APPKN670/671ML), or the Swedish and Arctic mutation in combination. The mAb158 sandwich ELISA analysis revealed presence of Aβ protofibrils in both cell and animal models, proving that Aβ protofibrils are formed not only in vitro, but also in vivo. Furthermore, elevated Aβ protofibril levels in the Arctic-Swedish samples emphasize the usefulness of the Arctic mutation as a model of enhanced protofibril formation. This assay provides a novel tool for investigating the role of Aβ protofibrils in AD and has the potential of becoming an important diagnostic assay.
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| 8. |
- Johansson, Ann, 1955-, et al.
(författare)
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A participatory evaluation of the health promotion programme “more healthy years of life” programme among senior citizens in Sweden
- 2018
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Ingår i: Cogent Medicine. - : Cogent OA. - 2331-205X. ; 5:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: Older adults have, in general, been sparsely involved in development and evaluation of programmes intended to promote their health.Aim: To describe older adults’ reflections on and involvement in the development and evaluation of a health promotion programme.Material and Method: Ten older persons participated in a health promotion programme (HPP) focusing on activity during four sessions. After each HPP session, focus group discussions were held, analysed through qualitative content analysis.Results: The main theme; “Being involved adds value and new experiences to life“, were built from sub-themes; “From sceptical individual to engaged group member”, “From beholder to active co-creator”, and “From individual knowledge recipient to collective knowledge sharer”.Conclusions: Having a leader with a gerontological competence was mentioned as important, as well as to integrate existential topics into the HPP. Social inclusion together with the possibility to influence the HPP had a positive effect on the participants and provided a sense of belonging.Significance: Several contributions to the development of the HPP were given, that would not have been captured without the reflections and involvement of the participants. However, more and larger studies are needed to develop strategies that enable older adult’s involvement in the development of HHP.
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