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- Johansson, Sofi, 1975
(författare)
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CC16 in allergy and allergic inflammation
- 2008
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Clara cell 16-kDa (CC16) is an anti-inflammatory protein mainly produced in the lung epithelium by Clara cells. Patients with asthma have lower levels of CC16 in bronchoalveolar lavage fluid and serum compared with healthy controls. In OVA-sensitised and challenged CC16-deficient mice, eosinophilia and the production of Th2 cytokines in the lung is higher compared with wild-type mice. Moreover, CC16 has been shown to inhibit cytokine production from a murine Th2 cell line and to inhibit the migration of rabbit neutrophils. CC16 also binds to the mast-cell derivative PGD2 and inhibits the stimulation of the DP1 receptor. For this reason, the first aim was to investigate whether CC16 levels in nasal lavage would be lower in children with allergic rhinitis compared with healthy controls. Our second aim was evaluate whether a low level of CC16 in plasma early in life is involved in the development of asthma, eczema and allergic rhinitis (ARC). Our third aim was to examine whether CC16 would inhibit Th2 differentiation and if CC16 would inhibit PGD2 and fMLF-induced eosinophil and neutrophil migration. CC16 was measured in nasal lavage samples from children with and without birch-pollen induced allergic rhinitis and serum samples from Icelandic children with or without RSV bronchiolitis. CC16 levels were also measured in plasma samples from a prospective birth cohort study at birth, and at four, 18 and 36 months. Clinical evaluations regarding the development of asthma, eczema and ARC were made at 36 months of age. Moreover, the effect of CC16 on Th2 differentiation was measured with an in vitro model for allergic T-cell sensitisation using human autologous neonatal mononuclear cells. The migration of eosinophils and neutrophils was assessed in a microplate migration system using specific ligands and receptor antagonists. We found that the levels of CC16 were significantly lower in nasal lavage fluid in children with birch-pollen-induced rhinitis compared with healthy controls both during and after the pollen season. Plasma levels of CC16 in children peaked at four months but we found no relationship between low levels of CC16 at any of the time points and the development of asthma, eczema or ARC. However, the CC16 serum levels were higher in children with RSV compared with healthy controls and we noted that the healthy Swedish children had significantly higher levels of CC16 in plasma compared with healthy Icelandic infants. CC16 did not inhibit cytokine production of human Th2 cells. However, CC16 was able to inhibit Th2 differentiation induced by birch pollen allergen via the dendritic cell. CC16 did not inhibit PGD2-induced eosinophil migration but CC16 inhibited the migration of both neutrophils and eosinophils towards fMLF. To conclude, levels of CC16 in plasma during the first years of life do not appear to be related to the development of asthma, eczema or allergic rhinitis. Instead, low levels of CC16 in asthmatic and allergic patients may be due to epithelial damage and the reduced re-growth of Clara cells. Reduced CC16 production may cause an increase in the allergic inflammatory response and thus lead to more severe asthma or allergy.
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| 2. |
- Johansson, Sofi, 1975, et al.
(författare)
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CC16 Inhibits the Migration of Eosinophils Towards the Formyl Peptide fMLF but not Towards PGD(2).
- 2009
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Ingår i: Inflammation. - : Springer Science and Business Media LLC. - 0360-3997 .- 1573-2576. ; 32:2, s. 65-9
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Tidskriftsartikel (refereegranskat)abstract
- Clara cell 16-kDa (CC16) is an anti-inflammatory protein chiefly produced in the lung epithelium. CC16 has been shown to inhibit the migration of rabbit neutrophils and human monocytes toward the formyl peptide N-formyl-methionine-leucin-phenylalanin (fMLF). Eosinophils migrate towards prostaglandin D2 (PGD(2)) and CC16 has been shown to bind to PGD(2). Therefore we investigated if CC16 could inhibit the migration of human eosinophils and neutrophils towards fMLF and/or PGD(2). Migration of eosinophils and neutrophils was assessed in a microplate migration system using specific ligands and receptor antagonists. CC16 inhibited the migration of eosinophils and neutrophils toward fMLF, which is likely to result from the interaction of CC16 with members of the formyl-peptide receptor family. However, CC16 did not inhibit eosinophil migration towards PGD(2). We therefore propose that CC16 may down-modulate the entry of human eosinophils and neutrophils into the airways during inflammation in the lung.
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| 3. |
- Johansson, Sofi, 1975, et al.
(författare)
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Clara cell 16-kd protein downregulates T(H)2 differentiation of human naive neonatal T cells
- 2007
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Ingår i: Journal of Allergy and Clinical Immunology. - : Elsevier BV. - 0091-6749. ; 120:2, s. 308-14
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Levels of the Clara cell 16-kd protein (CC16) are lower in plasma and bronchoalveolar lavage fluid from adults with asthma relative to those seen in healthy control subjects, and CC16 inhibits the T(H)2 cytokine production from murine T cells. OBJECTIVE: We sought to determine the plasma levels of CC16 in infants and to investigate whether CC16 might inhibit the T(H)2 cytokine production from neonatal T cells. METHODS: Cord blood and blood samples at 4, 18, and 36 months of age were taken from 64 children prospectively, and CC16 levels were analyzed in plasma. Cord monocyte-derived dendritic cells (DCs) were pulsed with birch allergen extract alone or together with CC16 or prostaglandin D(2) receptor inhibitors, after which autologous naive CD4(+) T cells were added to the DCs. The production of IL-5, IL-13, and IFN-gamma was measured by means of ELISA and flow cytometry. RESULTS: The plasma levels of CC16 in children peaked at 4 months. CC16 did not directly affect the cytokine production from human T(H)2 cells. However, CC16 inhibited birch pollen extract-stimulated T(H)2 differentiation of naive T cells through the DC. Inhibition of the prostaglandin D(2) receptors did not consistently result in suppressed T(H)2 differentiation. CONCLUSION: The production of CC16 seems to peak early in life, and CC16 has an inhibitory effect on T(H)2 cell differentiation from human infants by affecting DCs. CLINICAL IMPLICATIONS: CC16 is an immunoregulatory protein, and its inhibitory effect on T(H)2 cell differentiation might be of importance in the pathogenesis of allergy in infants.
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