| 1. |
- Field, Dawn, et al.
(författare)
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The minimum information about a genome sequence (MIGS) specification.
- 2008
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Ingår i: Nature biotechnology. - : Springer Science and Business Media LLC. - 1546-1696 .- 1087-0156. ; 26:5, s. 541-7
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Tidskriftsartikel (refereegranskat)abstract
- With the quantity of genomic data increasing at an exponential rate, it is imperative that these data be captured electronically, in a standard format. Standardization activities must proceed within the auspices of open-access and international working bodies. To tackle the issues surrounding the development of better descriptions of genomic investigations, we have formed the Genomic Standards Consortium (GSC). Here, we introduce the minimum information about a genome sequence (MIGS) specification with the intent of promoting participation in its development and discussing the resources that will be required to develop improved mechanisms of metadata capture and exchange. As part of its wider goals, the GSC also supports improving the 'transparency' of the information contained in existing genomic databases.
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| 2. |
- Granhag, Lena, 1974, et al.
(författare)
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The importance of bacterial film composition for germination of zoospores of the green alga Ulva
- 2014
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Ingår i: International Congress on Marine Corrosion and Fouling, Singapore 6-10 July 2014.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The green macroalga Ulva is a worldwide known and distributed fouling organism on ship hulls. The initial rate of germination is a critical process in macroalgae as it contributes to the success of colonization. The germination process is essential for the survival of settled zoospores and has consequences in relation to competition for space on surfaces with other fouling organisms. In this study the role of bacteria for germination and initial growth of zoospores from the green alga Ulva was investigated. The presence of a bacterial biofilms was seen to enhance germination. Further the influence of different bacteria biofilm composition was investigated using natural bacterial assemblages. The natural assemblages contained different proportions of Alphaproteobacteria, Gammaproteobacteria and Bacteriodetes and were analyzed/ characterized with the Fluorescent in Situ Hybridisation (FISH). More zoospore germination occurred on biofilms with a high proportion of Gammaproteobacteria. The possible consequences of different bacterial densities and community composition for Ulva spore germination are discussed.
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| 3. |
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| 4. |
- Tait, Karen, et al.
(författare)
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Disruption of quorum sensing in seawater abolishes attraction of zoospores of the green alga Ulva to bacterial biofilms.
- 2005
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Ingår i: Environ Microbiol. - 1462-2912. ; 7:2, s. 229-40
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Tidskriftsartikel (refereegranskat)abstract
- Zoospores of the eukaryotic green seaweed Ulva respond to bacterial N-acylhomoserine lactone (AHL) quorum sensing signal molecules for the selection of surface sites for permanent attachment. In this study we have investigated the production and destruction of AHLs in biofilms of the AHL-producing marine bacterium, Vibrio anguillarum and their stability in seawater. While wild type V. anguillarum NB10 was a strong attractor of zoospores, inactivation of AHL production in this strain by either expressing the recombinant Bacillus lactonase coding gene aiiA, or by mutating the AHL biosynthetic genes, resulted in the abolition of zoospore attraction. In seawater, with a pH of 8.2, the degradation of AHL molecules was temperature-dependent, indicating that the AHLs produced by marine bacterial biofilms have short half-lives. The Ulva zoospores sensed a range of different AHL molecules and in particular more zoospores settled on surfaces releasing AHLs with longer (>six carbons) N-linked acyl chains. However, this finding is likely to be influenced by the differential diffusion rates of AHLs from the experimental surface matrix. Molecules with longer N-acyl chains, such as N-(3-oxodecanoyl)- L-homoserine lactone, diffused more slowly than those with shorter N-acyl chains such as N-(3-hydroxy-hexanoyl)- L-homoserine lactone. Image analysis using GFP-tagged V. anguillarum biofilms revealed that spores settle directly on bacterial cells and in particular on microcolonies which we show are sites of concentrated AHL production.
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