| 1. |
|
|
| 2. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 3. |
- Högberg, Anders, 1968-, et al.
(författare)
-
Uppdragsarkeologiska möjligheter : Nya sätt att tänka om en samtidsverksamhet i en framtidsbransch
- 2021
-
Ingår i: In Situ Archaeologica. - Göteborg : University of Gothenburg. - 2000-4044 .- 2002-7656. ; 15, s. 5-30
-
Tidskriftsartikel (refereegranskat)abstract
- In this text we discuss how Swedish contract archaeology can develop its social engagement by creating new areas of relevance to society, beyond the general activities of disseminating results. We do so by giving concrete examples of how this can be done, using archaeological excavations as a starting point. The examples include engaging the local community in future planning for a social sustainable living environment, collaboration with the tourism sector, development of teaching materials for secondary schools, memory training for people with acquired brain damage, and school programs focusing on a socially sustainable and inclusive society. The purpose of the text is to inspire change, by showing development opportunities for future contract archaeology that will benefit both performers and recipients, and contribute to society’s multifaceted needs.
|
|
| 4. |
- Innala, Lena, 1060-, et al.
(författare)
-
Co-morbidity in patients with early rheumatoid arthritis - inflammation matters
- 2016
-
Ingår i: Arthritis Research & Therapy. - : BioMed Central. - 1478-6362. ; 18
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Patients with rheumatoid arthritis (RA) suffer from co-morbidities that contribute to a shortened lifespan. Inflammation is important for the development of cardiovascular disease, but little is known on its relationship with other co-morbidities. We investigated the role of inflammation for the development of new comorbidities in early RA. Methods: Since 1995, all patients with early RA in Northern Sweden are included in a prospective study on comorbidities, with a total of 950 patients being included. At the time for this study, 726 had been ill for >= 5 years. Data on co-morbidities, clinical and laboratory disease activity and pharmacological therapy were collected from patient records and further validated using a questionnaire at RA onset (T0) and after 5 years (T5). Results: Of the patients, 53.2 % of the patients had one or more co-morbidity at onset, the commonest being: hypertension (27.3 %), obstructive pulmonary disease (13.9 %), diabetes (8.0 %), hypothyroidism (6.3 %) and malignancy (5.0 %). After 5 years, 41.0 % had developed at least one new co-morbidity, the most common being: hypertension (15.1 %), malignancy (7.6 %), stroke/transient ischemic accident (5.1 %), myocardial infarction (4.3 %) and osteoporosis (3.7 %). Age at disease onset, a raised erythrocyte sedimentation rate (ESR) at inclusion, previous treatment with glucocorticoids (GC; p < 0.001 for all), extra-articular RA (Ex-RA; p < 0.01), DAS28 (area under the curve) at 24 months (p < 0.05), previous smoking at inclusion (p = 0.058) and male gender (p < 0.01) were associated with a new co-morbidity overall at T5. Treatment with biologics (p < 0.05) reduced the risk. In multiple logistic regression modelling, ESR (p = 0.036) at inclusion was associated with a new co-morbidity after 5 years, adjusted for age, sex, smoking and GC treatment. In a similar model, Ex-RA (p < 0.05) was associated with a new co-morbidity at T5. In a third model, adjusted for age and sex, a new pulmonary co-morbidity was associated with a smoking history at inclusion (p < 0.01), but not with ESR. Conclusion: There was substantial co-morbidity among early RA patients already at disease onset, with considerable new co-morbidity being added during the first five years. Measures of disease activity were associated with the occurrence of a new co-morbidity indicating that the inflammation is of importance in this context.
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
- Jonsson, Cecilia, 1974-, et al.
(författare)
-
Police cooperation with civil society: From a closed authority to an open social actor?
- 2019
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The Police has as its main task to prevent crime and increase safety and security. This cannot be done by the police force itself, but in collaboration with the surrounding society. An important purpose with the Swedish police reform in 2015 was that police work should be conducted closer to the citizens and the civil society. Essential in this policy is local policy work with a focus on cooperation regarding crime prevention with both the municipalities and other actors. The cooperation on crime in the local society is a way to increase knowledge and contact with the citizens and members of different groups and associations. This type of cooperation is scientifically a relatively unexplored area in Sweden. The main objective with this study is to map out the different types of cooperation between the police of the civil society to understand pros and cons with this cooperation. RQ1: In what way do the police get use of the cooperation with the civil society?RQ2: When and in what form is the cooperation conducted?RQ3: How is the cooperation conducted?In order to get a deeper understanding of different forms of collaborations this study is geographical delimited to one police area in the south of Sweden – Kronoberg/Kalmar. It is based on interviews with representatives of the Swedish police authority at the local level and non-profit associations cooperating with the police and participatory observation.The empirical findings show a wide range of cooperation forms such as established organizational cooperation and individual based connections. The results also suggest a tendency towards four ideal types of cooperation, which can be patterned in variations of:Function based collaboration, situation based collaboration, person based collaboration and organization based collaboration. There are also indications that the importance of this cooperation, stressed in policy documents and by the management, is not always shared with the police working within the operational activities. Implications and usefulness of these findings to future practice will be further explored.
|
|
| 8. |
- Jonsson, Cecilia, 1974-, et al.
(författare)
-
Polisens samverkan med civilsamhället
- 2021
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Samverkan som styrningsform lyfts ofta fram som lösningen på många olikaproblem inom offentlig sektor. I den här rapporten så intresserar sig författarnaför den samverkan som finns mellan Polisen och civilsamhällets organisationer,som utgörs av nätverk, föreningar, organisationer och trossamfund, det vill sägamänniskor med gemensamma intressen som gör något tillsammans på en arenasom är skild från den offentliga sektorn, näringslivet och det enskild hushållet.Att involvera civilsamhället i brottsförebyggande arbete är inget nytt feno-men, men trots det finns väldigt lite kunskapsunderlag om både former ochfunktion i Sverige. Vad bidrar till en framgångsrik samverkan, vilka utmaningaroch hinder finns det för samverkan mellan det civila samhället och Polismyn-digheten? Den här rapporten syftar till att bidra till en ökad kunskap om hurPolisens samverkan med civilsamhället kan ta sig uttryck.
|
|
| 9. |
- Rullander, Anna-Clara, et al.
(författare)
-
Adolescents' experience with Scoliosis Surgery : A Qualitative Study
- 2013
-
Ingår i: Pain Management Nursing. - : Elsevier. - 1524-9042 .- 1532-8635. ; 14:1, s. 50-59
-
Tidskriftsartikel (refereegranskat)abstract
- This article reports a study of adolescents' narrated experiences of undergoing scoliosis surgery. Six adolescents were interviewed. Open and semistructured questions were asked, and a qualitative content analysis of the text was performed. The results are presented in three main categories followed by subcategories. The three main categories of experience were emotional, physical, and social. The emotional aspects that emerged were fear, nightmares, nervousness, and helplessness. These had a great impact on adolescents' well-being before, during, and after the hospital visit. The physical aspects were mobilization, scars, different hip levels, pain, nausea, appetite, and urinary catheter. These aspects caused much discomfort, mostly during the hospital visit. The social aspects were friends, power, coaching and comfort, and sports. Some of the social aspects had a strong negative impact on the adolescents' well-being mostly after the hospital visit. This study suggests that both before and long after the surgery adolescents have strong emotions that they should be better prepared and helped to manage. To optimize perioperative care an interdisciplinary, a holistic approach must be taken that incorporates the complexity and whole of the adolescent's experiences. The findings of this study suggest that perioperative care of adolescents during scoliosis surgery needs to be optimized. To improve patients' psychologic preparation before surgery pediatric nurses should learn more about the individual patient and make care plans from a holistic perspective. Follow-up after discharge should address emotional, social, and physical aspects of the adolescent's health. (C) 2013 by the American Society for Pain Management Nursing
|
|
| 10. |
- Rullander, Anna-Clara, et al.
(författare)
-
Young People's Experiences With Scoliosis Surgery A Survey of Pain, Nausea, and Global Satisfaction
- 2013
-
Ingår i: Orthopedic Nursing. - 0744-6020 .- 1542-538X. ; 32:6, s. 327-333
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Scoliosis surgery is one of the most extensive elective surgical processes performed on young people. Although there is a great store of knowledge of surgical techniques, patients' experiences of going through surgery have not been extensively studied. PURPOSE: The aim of this study is to describe how a cohort of young people and their parents retrospectively rate postoperative pain and nausea and describe their experiences of scoliosis surgery. METHODS: In a retrospective cohort study, 87 young people aged 8-25 years with scoliosis who underwent corrective surgery from 2004 to 2007 were invited to complete a questionnaire, as were their parents. The semistructured questionnaire dealt with experiences of pain, nausea, and global satisfaction pre- and posthospitalization, assessed by visual analogue scales. The free text commentaries were analyzed using qualitative content analysis. RESULTS: A total of 51 patients (59%) and 65 parents (75%) answered the questionnaires. Out of the completed questionnaires, 41 had idiopathic, 23 neuromuscular, and 6 other types of scoliosis. Postoperative patient-rated pain was severe 7.3 (median, interquartile range 5-8.4, visual analogue scale 0-10 cm), and the severe pain lasted for 5 (median, 2.7-7.0) days. Nausea was rated to a median of 5 (1.1-7.3) and lasted for a median of 3 (1-5.2) days. Global satisfaction was rated to a median of 3.2 (1.5-5.2). Postoperative pain was the most prominent issue, and present pain was found in 51% of respondents. Nausea and loss of appetite were common during the entire hospital stay. Waiting for the nurses' assistance, lack of control, and technical failures with the analgesia equipment caused discomfort. Parents experienced a lack of confidence in the nurses and felt helpless to support their child or relieve the child's suffering. CONCLUSION: Young people who underwent scoliosis surgery reported severe postoperative pain and nausea during the hospitalization period and persistent and recent onset pain after discharge, although they did not indicate global dissatisfaction with the hospital stay.
|
|
