| 1. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 2. |
- Joleby, Malin, 1988, et al.
(författare)
-
"All of Me Is Completely Different" : Experiences and Consequences Among Victims of Technology-Assisted Child Sexual Abuse
- 2020
-
Ingår i: Frontiers in Psychology. - : Frontiers Media SA. - 1664-1078. ; 11
-
Tidskriftsartikel (refereegranskat)abstract
- The aim of the present study was to gain a first-person perspective on the experiences of technology-assisted child sexual abuse (TA-CSA), and a deeper understanding of the way it may affect its victims. Seven young women (aged 17-24) with experience of TA-CSA before the age of 18 participated in individual in-depth interviews. The interviews were teller-focused with the aim of capturing the interviewee's own story about how they made sense of their experiences over time, and what impact the victimization had on them in the short and long terms. Thematic analysis of the interviews revealed a broad range of abusive experiences that had profoundly impacted the individuals' lives, health and self-concepts. Three dominant themes emerged from the analysis - From thrilling to abusive, Negative effect on health and wellbeing, and A new self after the abuse. From thrilling to abusive captures the wide range of experiences described, starting from the child's own sexual curiosity to descriptions of having been manipulated or threatened into engaging in sexual activity, as well as the sometimes long and complex process of understanding the severity of one's experiences. Negative effect on health and wellbeing describes the victimization's comprehensive impact on the life and health of the participants, how they blamed themselves for what had happened, and the struggle of having to live with the constant fear of pictures from the abuse resurfacing. A new self after the abuse depicts how the victimization impacted the way participants viewed and thought about themselves in relation to others, and distorted their views of their bodies. The findings are discussed in relation to previous research on both offline CSA and TA-CSA, as well as theoretical and practical implications.
|
|
| 3. |
- Kaneryd, Linda, et al.
(författare)
-
Species-rich ecosystems are vulnerable to cascading extinctions in an increasingly variable world
- 2012
-
Ingår i: Ecology and Evolution. - : Wiley-Blackwell. - 2045-7758. ; 2:4, s. 858-874
-
Tidskriftsartikel (refereegranskat)abstract
- Global warming leads to increased intensity and frequency of weather extremes. Such increased environmental variability might in turn result in increased variation in the demographic rates of interacting species with potentially important consequences for the dynamics of food webs. Using a theoretical approach, we here explore the response of food webs to a highly variable environment.We investigate how species richness and correlation in the responses of species to environmental fluctuations affect the risk of extinction cascades. We find that the risk of extinction cascades increases with increasing species richness, especially when correlation among species is low. Initial extinctions of primary producer species unleash bottom-up extinction cascades, especially in webs with specialist consumers. In this sense, species-rich ecosystems are less robust to increasing levels of environmental variability than species-poor ones. Our study thus suggests that highly speciesrich ecosystems such as coral reefs and tropical rainforests might be particularly vulnerable to increased climate variability.
|
|
| 4. |
- Landberg, Åsa, 1963-, et al.
(författare)
-
Patterns of disclosure and perceived societal responses after child sexual abuse
- 2022
-
Ingår i: International Journal of Child Abuse & Neglect. - : Elsevier BV. - 0145-2134 .- 1873-7757. ; 134
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Disclosure of child sexual abuse (CSA) is key for abused children to access help and to protect them and other children.OBJECTIVE: The aim of this study was to investigate patterns of disclosure of child sexual abuse and how children perceived responses from people they disclosed to.PARTICIPANTS AND SETTING: Data were collected online in classrooms and during home studies in a national probability-based sample of 3282 third-year students in Swedish high schools in 2020-2021 (the age of the students varied between 16 and 23 years (M = 18,2)).METHODS: Results are presented with frequencies (n) and percentages (%). Pearson's chi-squared test was used for comparisons between groups.RESULTS: First, a substantial share of abused girls and most abused boys had not yet told anyone about the abuse, leaving them unable to access protection or rehabilitation. Second, participants who had disclosed sexual abuse had most often turned to a peer, more rarely to an adult, and seldom to a professional or volunteer. Third, although the societal responses that the participants perceived were mixed, more severe abuse was associated with more negative societal responses. Fourth, most participants stated that they did not need any professional support. Fifth, among the minority who had sought help, half were satisfied and a third dissatisfied.CONCLUSIONS: Results showed that most children with experience of CSA did not have access to the protection, support, and rehabilitation that they have a right to. Preventive measures need to target children and young people, while societal responses after CSA, especially severe CSA, need to be improved.
|
|
