| 1. |
- Hasséus, B., et al.
(författare)
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Immunotoxic effects of smokeless tobacco on the accessory cell function of rat oral epithelium
- 1997
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Ingår i: European Journal of Oral Sciences. - 0909-8836 .- 1600-0722. ; 105:1, s. 45-51
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Tidskriftsartikel (refereegranskat)abstract
- Smokeless tobacco (ST) is known to adversely effect the oral mucosa, but knowledge about the influence on immune defence is limited. Few studies have investigated the effect of ST on the local immune response. In the present study, we have assessed the effect of a crude Swedish moist snuff (SS) extract, alkaloids, and nitrosamines on T-cell mitogenic response to Con A using epithelial cells, including Langerhans cells, of the rat oral mucosa as accessory cells. SS extract at a concentration of 4% reduced the T-cell proliferation by 50% (IC50 = 4%). Pretreatment of either oral epithelial cells or T-cells with SS extract also gave a significant inhibition of T-cell proliferation. This effect was not obtained following preincubation with SS components as alkaloids and different tobacco-specific nitrosamines (TSNA). None of the tested compounds were found to possess any mitogenic properties. This in vitro study showed that SS extract can evoke an immunosuppressive effect on mitogen-driven T-cell proliferation using cells from oral epithelium as accessory cells. This effect was more pronounced when SS extract was employed compared to when the single SS components were used alone.
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| 2. |
- Simark-Mattsson, Charlotte, 1955, et al.
(författare)
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Distribution of interferon-gamma mRNA-positive cells in oral lichen planus lesions.
- 1998
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Ingår i: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. - 0904-2512. ; 27:10, s. 483-8
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Tidskriftsartikel (refereegranskat)abstract
- The present study investigated the potential involvement of interferon-gamma (IFN-gamma)-producing cells in the pathogenesis of oral lichen planus (OLP). On biopsies from 10 OLP patients, an in situ hybridization technique was employed to determine the topographical distribution of cells expressing IFN-gamma mRNA. It was estimated that approximately 1% or fewer lesional cells were IFN-gamma mRNA-positive. These cells were mainly encountered lining the basal membrane in a majority of the patients, or were in a few cases circumscribing the infiltrate, but were more seldom localized to the center of the lesion. A slightly higher, but not statistically significant, number of phytohemagglutinin (PHA)-induced IFN-gamma-producing cells, in vitro, was found in blood from 11 other OLP patients compared with blood from matched controls. Equal concentrations of IFN-gamma in supernatants from PHA-stimulated blood cells were detected in the two groups. Similarly, the IFN-gamma response towards C. albicans was alike in OLP and in healthy control (HC) blood cells, indicating normal immunological memory function in the OLP patients. A small set of cells with spontaneous IFN-gamma production was found in OLP and in HC peripheral blood. The data suggest that T-lymphocyte activation and cytokine production act locally and are not reflected in peripheral blood. The localization of the IFN-gamma mRNA-positive cells indicates that the antigenic peptides are presented at the periphery of the mononuclear cell infiltrate. Furthermore, the low frequency of IFN-gamma mRNA-positive cells in the lesions suggests that the disease is maintained by a small number of antigen-specific T cells.
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| 4. |
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| 5. |
- Bankvall, Maria, et al.
(författare)
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A family-based genome-wide association study of recurrent aphthous stomatitis
- 2020
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Ingår i: Oral Diseases. - : Wiley. - 1354-523X .- 1601-0825. ; 26:8, s. 1696-1705
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Tidskriftsartikel (refereegranskat)abstract
- © 2020 The Authors. Oral Diseases published by John Wiley & Sons Ltd Objectives: The aetiology of recurrent aphthous stomatitis (RAS) remains unknown. Individuals may share features of genetic susceptibility, and there may also be a hereditary component. The aim was to identify patterns of association and segregation for genetic variants and to identify the genes and signalling pathways that determine the risk of developing RAS, through a family-based genome-wide association study (GWAS). Subjects and methods: DNA was extracted from buccal swabs of 91 individuals in 16 families and analysed in an Illumina core exome single nucleotide polymorphism (SNP) array. A family-based association test (dFAM) was used to derive SNP association values across all chromosomes. Results: None of the final 288,452 SNPs reached the genome-wide significant threshold of 5×10–8. The most significant pathways were the Ras and PI3K-Akt signalling pathways, pathways in cancer, circadian entrainment and the Rap 1 signalling pathway. Conclusions: This confirms that RAS is not monogenic but results as a consequence of interactions between multiple host genes and possibly also environmental factors. The present approach provides novel insights into the mechanisms underlying RAS and raises the possibility of identifying individuals at risk of acquiring this condition.
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| 6. |
- Bankvall, Maria, et al.
(författare)
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The engagement of oral-associated lymphoid tissues during oral versus gastric antigen administration
- 2016
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Ingår i: Immunology. - : Wiley. - 0019-2805. ; 149:1, s. 98-110
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Tidskriftsartikel (refereegranskat)abstract
- The role of oral-associated lymphoid tissues during induction of oral tolerance still remains elusive. Therefore, the aim was to compare T-cell activation and induction of tolerance to ovalbumin (OVA) presented through either of two routes; deposited into the oral cavity, or the stomach, thereby bypassing the oral cavity. OVA was administered by the oral or gastric route to BALB/c mice that had received OVA-specific DO11.10+ CD4(+) T cells, stained with CellTrace Violet dye, through intravenous injection. Proliferating OVA-specific T cells were detected in the nose-associated lymphoid tissues (NALT) and the cervical, mesenteric and peripheral lymph nodes at different time-points following OVA exposure. OVA-specific T-cell proliferation was initially observed in the NALT 1hr after oral, but not gastric, administration. However, at day 1, proliferation at this site was also detected after gastric administration and profound proliferation was observed at all sites by day 4. For the oral route the degree of proliferation observed was lower in the peripheral lymph nodes by day 4 compared with the other sites. These results demonstrate a similar activation pattern achieved by the two routes. However, the NALT distinguishes itself as a site of rapid T-cell activation towards fed antigens irrespective of feeding regimen. To evaluate induction of tolerance a semi-effective OVA dose was used, to detect differences in the degree of tolerance achieved. This was performed in a model of OVA-induced airway hypersensitivity. No differences in tolerance induction were observed between the two administration routes.
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| 7. |
- Bankvall, Maria, et al.
(författare)
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The oral microbiota of patients with recurrent aphthous stomatitis.
- 2014
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Ingår i: Journal of oral microbiology. - : Informa UK Limited. - 2000-2297. ; 6
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Tidskriftsartikel (refereegranskat)abstract
- Specific pathogenic bacteria have been implicated in recurrent aphthous stomatitis (RAS), a chronic inflammatory condition characterised by ulcerations in the oral mucosa. However, the aetiology behind this condition still remains unclear.
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| 8. |
- Bankvall, Maria, et al.
(författare)
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Tissue-specific Differences in Immune Cell Subsets Located in the Naso-oropharyngeal-associated Lymphoid Tissues
- 2018
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Ingår i: Scandinavian Journal of Immunology. - : Wiley. - 0300-9475. ; 87:1, s. 15-27
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Tidskriftsartikel (refereegranskat)abstract
- Defining the immune cells within the naso-oropharyngeal-associated lymphoid tissues would promote the development of efficient orally and nasally delivered immunotherapies. The aim was to compare murine antigen-presenting cells (APCs) and T cell subsets in the nose-associated lymphoid tissues (NALT), cervical lymph nodes (CLN), mesenteric lymph nodes (MLN) and peripheral lymph nodes (PLN) using flow cytometry and in vitro proliferation assays. Overall, the NALT contained a higher proportion of APCs and a lower proportion of T cells compared to the CLN, MLN and PLN. The APCs of the NALT more often belonged to the CD11c(+)CD11b(+) and the CD11c(neg)CD11b(+) subsets as compared to the other sites. Both of these APC populations showed little sign of activation, that is low expression of the markers CD40, CD86 and IAd. Instead, the APCs of the NALT more often co-expressed CX3CR1 and CD206, markers associated with a tolerogenic function. No increase in the proportion of regulatory T cells was observed in the NALT. Instead, the T cells frequently exhibited a memory/effector phenotype, expressing the homing markers 47, CCR4 and CCR9, but rarely the naive phenotype cell surface marker CD45RB. In contrast, the T cells at the other sites were mostly of the naive phenotype. In addition, cells from the NALT did not proliferate upon in vitro stimulation with Con A, whereas the cells from the other sites did. Taken together, these results suggest that the NALT is primarily an effector site rather than one for activation and differentiation, despite it being regarded as a site of induction.
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| 9. |
- Brailo, V., et al.
(författare)
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World Workshop on Oral Medicine VI: Utilization of Oral Medicine-specific software for support of clinical care, research, and education: current status and strategy for broader implementation
- 2015
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Ingår i: Oral Surgery Oral Medicine Oral Pathology Oral Radiology. - : Elsevier BV. - 2212-4403. ; 120:2, s. 172-184
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Tidskriftsartikel (refereegranskat)abstract
- Objectives. To assess the current scope and status of Oral Medicine-specific software (OMSS) utilized to support clinical care, research, and education in Oral Medicine and to propose a strategy for broader implementation of OMSS within the global Oral Medicine community. Study Design. An invitation letter explaining the objectives was sent to the global Oral Medicine community. Respondents were interviewed to obtain information about different aspects of OMSS functionality. Results. Ten OMSS tools were identified. Four were being used for clinical care, one was being used for research, two were being used for education, and three were multipurpose. Clinical software was being utilized as databases developed to integrate of different type of clinical information. Research software was designed to facilitate multicenter research. Educational software represented interactive, case-orientated technology designed for clinical training in Oral Medicine. Easy access to patient data was the most commonly reported advantage. Difficulty of use and poor integration with other software was the most commonly reported disadvantage. Conclusions. The OMSS presented in this paper demonstrate how information technology (IT) can have an impact on the quality of patient care, research, and education in the field of Oral Medicine. A strategy for broader implementation of OMSS is proposed.
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