| 1. |
- Ericson, Marica B, 1974, et al.
(författare)
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Imaging of Photosensitizers in Skin
- 2017
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Ingår i: Imaging in Photodynamic Therapy. Eds: Michael R. Hamblin, Yingying Huang. - Boca Raton : Taylor & Francis Group, CRC Press. - 9781498741453
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 2. |
- Inci, Rahime, 1985, et al.
(författare)
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Porokeratosis is one of the most common genodermatoses and is associated with an increased risk of keratinocyte cancer and melanoma
- 2023
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Ingår i: Journal of the European Academy of Dermatology and Venereology. - : Wiley. - 0926-9959 .- 1468-3083. ; 37:2, s. 420-427
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Tidskriftsartikel (refereegranskat)abstract
- Background Porokeratosis is a clinically heterogeneous group of keratinization disorders with a genetic background mainly affecting the mevalonate pathway, which is involved in the synthesis of cholesterol, an essential component for the formation of the extracellular lipid lamellae in the stratum corneum. Porokeratosis is reportedly associated with an increased risk of keratinocyte cancer, but to date, no large epidemiological studies have been conducted to further address this association. Objectives The first objective was to characterize a cohort of patients diagnosed with porokeratosis at the Department of Dermatology and Venereology, Sahlgrenska University Hospital (SU), Gothenburg, Sweden. The second objective was to conduct a nationwide registry-based cohort study to investigate the association, if any, between porokeratosis and the cutaneous malignancies squamous cell carcinoma (SCC), basal cell carcinoma (BCC) and melanoma. Methods For the SU cohort, the hospital registry was searched for patients with a diagnosis of porokeratosis recorded between 2016 and 2020. Clinical data were extracted from the records of the identified patients. For the nationwide cohort, national registries were searched to identify patients with a diagnosis of porokeratosis between 2001 and 2020. A tenfold control cohort was formed by Statistics Sweden. The data was cross-referenced with the Swedish Cancer Register to study the associations between porokeratosis and SCC, BCC and melanoma. Results Disseminated superficial actinic porokeratosis was the most common clinical type among the 108 patients in the SU cohort. In the nationwide search, 2277 patients with porokeratosis were identified (prevalence 1/4132). Porokeratosis was associated with an increased risk for SCC, BCC and melanoma with hazard ratios (95% CI) of 4.3 (3.4-5.4), 2.42 (1.97-2.98) and 1.83 (1.18-2.82), respectively, in the patient cohort, compared to the matched control group. Conclusion Porokeratosis is a common genodermatosis, and it is associated with an enhanced risk of skin cancer.
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| 3. |
- James, Jeemol, et al.
(författare)
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Report on fluorescence lifetime imaging using multiphoton laser scanning microscopy targeting sentinel lymph node diagnostics.
- 2020
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Ingår i: Journal of biomedical optics. - 1560-2281. ; 25:7, s. 1-8
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Tidskriftsartikel (refereegranskat)abstract
- Sentinel lymph node (SLN) biopsy is an important method for metastasis staging in, e.g., patients with malignant melanoma. Tools enabling prompt histopathological analysis are expected to facilitate diagnostics; optical technologies are explored for this purpose.The objective of this exploratory study was to investigate the potential of adopting multiphoton laser scanning microscopy (MPM) together with fluorescence lifetime analysis (FLIM) for the examination of lymph node (LN) tissue ex vivo.Five LN tissue samples (three metastasis positive and two negative) were acquired from a biobank comprising tissues from melanoma patients. Tissues were deparaffinized and subjected to MPM-FLIM using an experimental MPM set-up equipped with a time correlated single photon counting module enabling FLIM.The data confirm that morphological features similar to conventional histology were observed. In addition, FLIM analysis revealed elevated morphological contrast, particularly for discriminating between metastatic cells, lymphocytes, and erythrocytes.Taken together, the results from this investigation show promise for adopting MPM-FLIM in the context of SLN diagnostics and encourage further translational studies on fresh tissue samples.
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| 4. |
- Kantere, Despoina, et al.
(författare)
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Anti-Stokes fluorescence from endogenously formed protoporphyrin IX - Implications for clinical multiphoton diagnostics.
- 2013
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Ingår i: Journal of biophotonics. - : Wiley. - 1864-0648 .- 1864-063X. ; 6:5, s. 409-415
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Tidskriftsartikel (refereegranskat)abstract
- Multiphoton imaging based on two-photon excitation is making its way into the clinics, particularly for skin cancer diagnostics. It has been suggested that endogenously formed protoporphyrin IX (PpIX) induced by aminolevulinic acid or methylaminolevulinate can be applied to improve tumor contrast, in connection to imaging of tissue autofluorescence. However, previous reports are limited to cell studies and data from tissue are scarce. No report shows conclusive evidence that endogenously formed PpIX increases tumor contrast when performing multiphoton imaging in the clinical situation. We here demonstrate by spectral analysis that two-photon excitation of endogenously formed PpIX does not provide additional contrast in superficial basal cell carcinomas. In fact, the PpIX signal is overshadowed by the autofluorescent background. The results show that PpIX should be excited at a wavelength giving rise to one-photon anti-Stokes fluorescence, to overcome the autofluorescent background. Thus, this study reports on a plausible method, which can be implemented for clinical investigations on endogenously formed PpIX using multiphoton microscopy (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
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| 5. |
- Kantere, Despoina, et al.
(författare)
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Clinical Features, Complications and Autoimmunity in Male Lichen Sclerosus.
- 2017
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Ingår i: Acta dermato-venereologica. - : Medical Journals Sweden AB. - 1651-2057 .- 0001-5555. ; 97:3, s. 365-369
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Tidskriftsartikel (refereegranskat)abstract
- Lichen sclerosus is a chronic inflammatory disease associated with substantial morbidity. Knowledge of the aetiology and progression of lichen sclerosus is therefore needed. In this cross-sectional study, 100 male patients diagnosed with lichen sclerosus were interviewed and examined. Since there is a possible link between lichen sclerosus and autoimmunity, blood tests were analysed for thyroid disease, antinuclear antibodies and antibodies to extracellular matrix protein 1, but autoimmunity was found to be infrequent. In 72 participants active genital lichen sclerosis was observed and complications were common; 27 patients had preputial constriction and 12 meatal engagement. In total, 13 patients needed a referral to the Department of Urology, including 1 patient with suspected penile cancer. In conclusion, despite available treatment with ultra-potent steroids and circumcision, LS in males is frequently complicated by phimosis and meatal stenosis. However, the disease can also go into remission, as seen in 27% of our patients.
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| 6. |
- Kantere, Despoina
(författare)
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Diagnostic aspects of Lichen sclerosus and skin cancer studied with laser scanning microscopy
- 2020
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Ingår i: Forum for Nordic Dermato-Venerology. - 1402-2915. ; 25:3, s. 23-24
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Despoina Kantere conducted her PhD studies at the Department of Dermatology and Venereology, Sahlgrenska University Hospital, Gothenburg, Sweden during the period 2010–2020. Ann-Marie Wennberg Larkö was her main supervisor, and the co-supervisors were Professor Marica B. Ericson and Associate Professor Petra Tunbäck. The opponent was Professor Merete Haedersdal, University of Copenhagen, Copenhagen, Denmark. Complete dissertation can be found at https://gupea.ub.gu.se/handle/2077/64517. © 2020, Medical Journals/Acta D-V. All rights reserved.
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| 7. |
- Kantere, Despoina
(författare)
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Diagnostic aspects of skin cancer and lichen sclerosus studied with laser scanning microscopy
- 2020
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Histopathologic examination of tissue biopsies is the current gold standard for the diagnosis of dermatological problems. Similarly, in oncology, sentinel lymph node (SLN) biopsy is the state-of-the-art diagnostic method for me- tastasis screening. Although these methods are safe, they are associated with some limitations, particularly because they are invasive, labor-intensive, and time-consuming. Moreover, histopathological analysis does not always lead to conclusive results. Therefore, there is a need for the development of fast, accurate, and non-invasive diagnostic procedures, complementary to these standard techniques. It seems that laser scanning optical microscopy moda- lities have the potential to meet this need. Regarding this, the present study was conducted to explore the efficiency of two of these methods, namely re- flectance confocal microscopy (RCM) and multiphoton microscopy (MPM), in dermatological and oncological applications. Particular focus was given to lichen sclerosus (LS), basal cell carcinoma (BCC), and malignant melanoma (MM) metastases, all of which are important disorders requiring improved diagnostic methods. This thesis builds upon the work reported in five papers. The first two papers involved the investigation of LS. In the first paper, we reported the clinical signs of LS, namely hypopigmentation, petechiae, and preputial constriction, based on which the diagnosis of LS was established. In the se- cond paper, it was found that RCM could visualize the thick fiber structures corresponding to sclerosis in the dermis, thereby facilitating the differentia- tion of LS from normal penile skin. In the third paper, it was observed that the application of methyl-aminolaevulinic acid (MAL) on BCCs could not increa- se the contrast when imaged with ex vivo MPM. Furthermore, it was found that MAL-induced fluorescence cannot be excited by the expected two-pho- ton excitation route when studied with MPM; rather, a one-photon process (i.e., anti-Stokes fluorescence) takes place. This finding is important not only for diagnostic aspects but also for future work in which photodynamic effects might be required. The fourth and fifth papers involved the investigation of MM metastases in lymph node tissues. It was found that particularly ex vivo MPM can enable the visualization of the characteristic morphologic features in this type of tissue. Furthermore, by extending the spectroscopic informa- tion to include also fluorescence life-time, the latter has the potential to in- crease the diagnostic ability. Taken together, the obtained results were indicative of the potential of laser scanning microscopy techniques as diagnostic tools for the detection of LS, BCCs, and MM metastases to lymph nodes. Future studies are encouraged to fully explore the potential of these techniques to be used for dermatological and oncological investigations in a non-invasive/intravital manner.
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| 8. |
- Kantere, Despoina, et al.
(författare)
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Exploring reflectance confocal microscopy as a non-invasive diagnostic tool for genital lichen sclerosus.
- 2022
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Ingår i: Experimental and therapeutic medicine. - : Spandidos Publications. - 1792-0981 .- 1792-1015. ; 23:6
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Tidskriftsartikel (refereegranskat)abstract
- The diagnosis of genital lichen sclerosus (LS) is often confirmed by obtaining a skin biopsy, which can lead to unwanted complications and is uncomfortable in the sensitive genital area. Thus, there is a need of finding novel, non-invasive techniques that can rapidly and accurately diagnose LS. The present study investigated the potential for reflectance confocal microscopy (RCM) to diagnose LS compared with healthy penile skin and other common penile skin disorders in males. A total of 30 male patients, including patients with LS, nonspecific balanoposthitis, plasma cell balanitis and psoriasis, and healthy individuals were included and were subject to non-invasive RCM investigation. Prominent fiber-like structures, representing hyaline sclerosis, were observed in the RCM images for almost half of the patients. Differences between healthy penile skin and LS were confirmed by identifying the edged papillae on healthy skin and their absence or obscureness in patients with LS. Notably, RCM could detect the atypical honeycomb pattern referring to dysplasia in 1 patient with LS with penile intraepithelial neoplasia. In conclusion, the present study demonstrated that RCM can detect sclerosis in penile LS. RCM can potentially become a valuable tool for monitoring patients with LS for dysplasia providing a useful non-invasive diagnostic tool for genital disorders.
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| 9. |
- Kantere, Despoina, et al.
(författare)
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Label-free laser scanning microscopy targeting sentinel lymph node diagnostics: A feasibility study ex vivo
- 2020
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Ingår i: Translational Biophotonics. - : Wiley. - 2627-1850. ; 2:3
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Tidskriftsartikel (refereegranskat)abstract
- This proof-of-concept, observational study investigates the potential for label-free, laser scanning microscopy to identify melanoma metastatic features in lymph node tissues ex vivo - exploring the translational potential targeting sentinel lymph node diagnostics. Ten lymph node samples, of which seven are positive for melanoma metastases and three negative, were obtained from a tissue biobank. Imaging data acquired using multiphoton microscopy ex vivo were compared with histopathological findings. Morphologic features characteristic for histopathologic diagnosis of melanoma metastasis were confirmed in the positive samples. Four of the samples were complementary studied with reflectance mode confocal microscopy; however, visualization was found restricted to fibrous structures in this case. To conclude, the results imply that multiphoton microscopy based on autofluorescence is a promising label-free technique for visualization of cellular features in lymph node tissues.
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| 10. |
- Zagoras, Theofanis, et al.
(författare)
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Incidence and Prevalence of 73 Different Genodermatoses: A Nationwide Study in Sweden
- 2023
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Ingår i: Acta Dermato-Venereologica. - 0001-5555. ; 103
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Tidskriftsartikel (refereegranskat)abstract
- This retrospective registry-based cohort study aimed to estimate the incidence and prevalence of genodermatoses in the Swedish population and to analyse associated healthcare usage. Patients diagnosed with genodermatoses were identified from the patient registry of Sahlgrenska University Hospital (Gothenburg, Sweden) between 2016 and 2020. Clinical data from medical records were used to verify diagnoses recorded in the National Patient Registry (NPR). The NPR was then searched for International Classification of Diseases, Tenth Revision (ICD-10) codes Q80-82 and Q84 from 2001 to 2020. The local cohort included 298 patients with 36 unique genodermatosis diagnoses. Verification of these diagnoses in the NPR showed positive predictive values of over 90%. The NPR search yielded 13,318 patients with 73 unique diagnoses, including ichthyoses (n = 3,341; 25%), porokeratosis (n = 2,277; 17%), palmoplantar keratodermas (n = 1,754; 13%), the epidermolysis bullosa group (n = 1011; 7%); Darier disease (n = 770; 6%), HaileyHailey disease (n = 477; 4%) and Gorlin syndrome (n = 402; 3%). The incidence and prevalence of each diagnosis were calculated based on the nationwide cohort and are reported. A total of 149,538 outpatient visits were registered, a mean of 4.6 visits per patient. This study provides a valuable resource for the epidemiology of genodermatoses by reporting on the incidence and prevalence of 73 different genodermatoses.
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