| 1. |
- Enlund, Karolina Brunius, et al.
(författare)
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Professional dental cleaning in dogs: clinical routines among Swedish veterinarians and veterinary nurses
- 2020
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Ingår i: Acta Veterinaria Scandinavica. - : Springer Science and Business Media LLC. - 1751-0147 .- 0044-605X. ; 62:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: Dental disease is very common in dogs and veterinary professional dental cleaning and examination, together with daily dental home care, is the foundation for good dental health. To our knowledge, no previous study has investigated professional dental cleaning routines in small animal veterinary practice. A validated questionnaire survey was distributed to all veterinarians and veterinary nurses with registered e-mail addresses in the Swedish national registry (veterinarians; n = 3657, veterinary nurses; n = 1650). Response rates were 32% for veterinarians (V) and 38% for veterinary nurses (VN). Results: In total, 73% (V)/96% (VN) of respondents reported that professional dental cleaning was performed at their work place under general anesthesia, and 27% (V)/18% (VN) that dental cleaning was performed under sedation. Of the respondents, 43% (V)/96% (VN) considered regular dental cleaning under general anesthesia fairly or very important, and 49% (V)/47% (VN) stated that it was sometimes important for good dental health in dogs. A majority of respondents, 84% (V)/97% (VN), reported that dental extractions were performed at their clinic, and 72% (V)/90% (VN) had access to dental radiography equipment. Conclusion: A majority of Swedish veterinarians and veterinary nurses perform professional dental cleaning under general anesthesia with access to dental radiography equipment, in accordance with national and international recommendations. However, a considerable proportion of professional dental cleanings were performed under sedation only, and extractions performed without access to dental radiography equipment were common, suggesting several areas of improvement in the routines in Swedish veterinary clinics and hospitals. Our results clearly indicate the need for improved educational efforts to increase the awareness among veterinary health professionals regarding guidelines and official recommendations in canine dental care.
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| 2. |
- Furbo, Simon, et al.
(författare)
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Nordic Energy Research Cooperation on Solar Combisystems
- 2006
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Ingår i: Eurosun 2006. - Glasgow, UK.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- This paper describes ongoing research on solar combisystems for the Nordic and Baltic countries carried out within the projects "Competitive solar heating systems for residential buildings" and "Solar thermal components adapted to common building standards". The aim of the projects is to develop competitive solar combisystems which are attractive to buyers and to educate experts in the solar heating field.
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| 3. |
- Hertting, Krister, 1972-, et al.
(författare)
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Ping pong health! : A table tennis intervention for improved health at the workplace
- 2018
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Ingår i: The Science and Practice of Racket Sport for Improved Performance and Health: Special Focus on Table Tennis. - Halmstad : Halmstad University. - 9789163971297 ; , s. 22-23
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Konferensbidrag (refereegranskat)abstract
- Introduction: Physical activity has a positive impact on physical and psychological well-being (Delisle, Werch, Wong, Bian, & Weiler, 2010), as well as social relations and skills development (Lamu & Olsen, 2016; Perkins & Williamon, 2014). Adopting a “healthy organization” culture through health programs, with strong senior and middle management support, and using interventions can promote health at workplaces (Rajaratnam et. al., 2014). We, therefore, consider it relevant to have physical activity, social relations and skill development as the starting point in a tabletennis intervention at the workplace.Aim: The aim was to develop, implement, and evaluate an intervention for improving health and wellbeing in the workplace by introducing table tennis.Methods and results: The intervention design consisted of two workshops and five table tennis sessions. Thirteen employees from a warehouse within the retail sector participated in the intervention. The participants had various backgrounds in table tennis and sport in general, different motives to participate, and came from different groups of employees (management and stock). After an introductory workshop, two table tennis coaches held one training session a week over the fiveweek intervention period. The employees were divided in to two groups and each group had a 45-minute session. The evaluation is in progress at present. Pre- and post-measurement has been conducted using health questionnaires Short Form 36 Health Survey (SF-36) and International Physical Activity Questionnaire (IPAQ). These will be analysed using a Bayesian paired t-test analysis. Based on the results of the questionnaire focus group interviews will be conducted with participants. Three focus groups of 4-5 participants in each group. The questions will focus on 24 experiences of the intervention and reflections on future directions for table-tennis and wellbeing activities at the workplace. Finally, there will be a workshop pointing out future directions for sport-based health activities at the workplace.
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| 4. |
- Justus, Eugen, et al.
(författare)
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Synthesis, liposomal preparation, and in vitro toxicity of two novel dodecaborate cluster lipids for boron neutron capture therapy
- 2007
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Ingår i: Bioconjugate chemistry. - : American Chemical Society (ACS). - 1043-1802 .- 1520-4812. ; 18:4, s. 1287-1293
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Tidskriftsartikel (refereegranskat)abstract
- A new class of lipids, contg. the closo-dodecaborate cluster, has been synthesized. Two lipids, S-(N, N-(2-dimyristoyloxyethyl)acetamido)thioundecahydro-closo-dodecaborate (2-) (B-6-14) and S-(N, N-(2-dipalmitoyloxyethyl)acetamido)thioundecahydro-closo-dodecaborate (2-) (B-6-16) are described. Both of them have a double-tailed lipophilic part and a headgroup carrying two neg. charges. Differential scanning calorimetry shows that B-6-14 and B-6-16 bilayers have main phase transition temps. of 18.8 and 37.9 DegC, resp. Above the transition temp. of 18.8 DegC, B-6-14 can form liposomal vesicles, representing the first boron-contg. lipid with this capability. Upon cooling below the transition temp., stiff bilayers are formed. When incorporated into liposomal formulations with equimolar amts. of distearoyl phosphatidylcholine (DSPC) and cholesterol, stable liposomes are obtained. The z-potential measurements indicate that both B-6-14- and B-6-16-contg. vesicles are neg. charged, with the most neg. potential described of any liposome so far. The liposomes are of high potential value as transporters of boron to tumor cells in treatments based on boron neutron capture therapy (BNCT). Liposomes prepd. from B-6-14 were slightly less toxic in V79 Chinese hamster cells (IC50 5.6 mM) than unformulated Na2B12H11SH (IC50 3.9 mM), while liposomes prepd. from B-6-16 were not toxic even at 30 mM.
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| 5. |
- Karlsson, Charlie, 1945-, et al.
(författare)
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ICT and Regional Economic Dynamics : A Literature Review
- 2010
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The objective of this report is to review the relevant theoretical and empirical literature to provide a conceptual and methodological background for the analysis of the consequences of ICT use and globalisation on the regional economies in the European Union. We highlight the key aspects of ICT as a general purpose technology, discuss the economic impacts of ICT diffusion from macro and micro perspectives, and examine the spatial consequences of ICT diffusion. We focus on regional innovation systems and globalisation in order to propose an organizing framework for the analysis of the impact of ICT diffusion on regional development.
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| 6. |
- Karlsson, Michaela Elisabeth, et al.
(författare)
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“Knowing That This Is My Place Is Very Positive” : The Case of a Swedish Table Tennis Club
- 2021
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Ingår i: Case Studies in Sport and Exercise Psychology. - Champaign, IL : Human Kinetics. - 2470-4849 .- 2470-4857. ; 5:1, s. 154-163
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Tidskriftsartikel (refereegranskat)abstract
- This case study is guided by the holistic ecological approach and aimed at (a) providing a holistic description of an athletic talent development environment using a table tennis club in Sweden as a case study and (b) examining the factors perceived as influential to the effectiveness of the club’s talent development. The holistic ecological approach’s two working models informed the data collection (through interviews, observation, and analysis of documents) and were subsequently transformed into empirical models, acting as a summary of the case. Findings revealed that the environment’s success in talent development can be seen as an outcome of the following key features: (a) flexible and supportive training groups, (b) opportunities to learn from senior elite athletes, (c) support through the club and sport-friendly schools, (d) support of the development of psychosocial skills, (e) regular and intensive training, (f) focus on long-term development and athletes as whole persons, (g) strong and coherent organizational culture centered around the basic assumption, “we are a community of committed members,” and (h) integrated efforts among the club and sport-friendly schools to support athletes’ development. This case study can inform other athletic talent development environments on how to optimize talent development processes.
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| 7. |
- Nejedla, Michaela, et al.
(författare)
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A Fluorophore Fusion Construct of Human Profilin I with Non-Compromised Poly(L-Proline) Binding Capacity Suitable for Imaging
- 2017
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Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836 .- 1089-8638. ; 429:7, s. 964-976
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Tidskriftsartikel (refereegranskat)abstract
- Profilin is vital for actin organisation in eukaryotic cells. It controls actin filament formation by binding monomeric actin and numerous proteins involved in polarised actin assembly. Important for the latter is the interaction surface formed by the N- and C-terminal helices, which pack close to each other on one side of the molecule at a distance from the actin site and mediate binding to poly-proline sequences present in many of the targeted proteins. Via these interactions, profilin contributes to the spatiotemporal control of actin filament growth. Studies of profilin dynamics in living cells by imaging techniques have been hampered by problems to generate fusion constructs with fluorophore proteins without negatively impacting on its poly-proline binding. With the object to circumvent this problem, we have generated an internal fusion of profilin with the green fluorescent variant citrine, here referred to as citrine profilin. The characterisation of citrine profilin (CIT-Pfn) demonstrates that it has full capacity to interact with poly-proline and also binds phosphatidylinositol lipids and actin, albeit with 10 times reduced affinity for the latter. Imaging of living cells expressing CIT-Pfn showed a distribution of the fusion protein similar to endogenous profilin. Furthermore, CIT-Pfn rescued the phenotypes observed after the Crispr/Cas9 knockout of the profilin 1 gene, including the lost migratory capacity characterising the knockout cells. Based on this, we conclude that the CIT-Pfn construct will be useful as a tool for displaying profilin localisation in living cells and obtaining information on its dynamic organisation under different conditions and activations of the actin microfilament and microtubule systems.
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| 8. |
- Nejedla, Michaela, et al.
(författare)
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Profilin connects actin assembly with microtubule dynamics
- 2016
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Ingår i: Molecular Biology of the Cell. - 1059-1524 .- 1939-4586. ; 27:15, s. 2381-2393
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Tidskriftsartikel (refereegranskat)abstract
- Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making actin polymerization from microtubule-associated platforms possible. Microtubules are implicated in focal adhesion turnover, cell polarity establishment, and migration, illustrating the coupling between actin and microtubule systems. Here we demonstrate that profilin is functionally linked to microtubules with formins and point to formins as major mediators of this association. To reach this conclusion, we combined different fluorescence microscopy techniques, including superresolution microscopy, with siRNA modulation of profilin expression and drug treatments to interfere with actin dynamics. Our studies show that profilin dynamically associates with microtubules and this fraction of profilin contributes to balance actin assembly during homeostatic cell growth and affects microtubule dynamics. Hence profilin functions as a regulator of microtubule (+)-end turnover in addition to being an actin control element.
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| 9. |
- Nejedla, Michaela, 1986-
(författare)
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Profilins : The Control of the Microfilament and Microtubule systems
- 2017
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The microtubule and actin filament systems are important for many cellular processes such as intracellular organelle trafficking, cell adhesion, migration and mitosis. The two filament systems are closely connected, operate in parallel and are essential for proper cell function. This thesis demonstrates that profilin, an actin monomer binding protein that enhances actin nucleotide exchange, is central for actin polymerization control and binds poly-proline sequences present in many actin nucleation and elongation promoting factors,also contributes to the coordination of the actin and microtubule systems.The results show that profilin is linked to microtubules via members of the formin family of proteins. This was realized by combining different microscopy techniques with biochemical analyses. Exposure of the cells to the formin inhibitor SMIFH2 led to a significant decrease of the profilin-microtubule co-distribution, and this was also observed by simultaneously decreasing expression of two formins, Diaphaneous 1 and 2, by siRNA-transfection. Together this provided strong evidence for the formins as major components associating profilin to the microtubules. With the use of actin-targeted drugs, I further showed that the turn-over of the actin microfilament system balances the extent of profilin-microtubule association, and, moreover, down-regulation of profilin expression resulted in increased levels of acetylated tubulin and faster microtubule elongation. All in congruence with profilin being central in coordinating the activities of the microtubule and actin microfilament systems.In some of the experiments above, I used a specifically developed fluorescent variant of profilin that carries the fluorescent protein citrine internally in a loop extending away from the actin and proline-binding surfaces. In a separate study it was shown to bind poly-proline, phosphatidylinositol lipids and actin, and also to distribute properly in cells after expression. Furthermore, this fusion-construct largely rescued the phenotypes displayed by a Crispr/Cas 9 profilin I gene knock-out, and therefore opens for detailed future analyses of profilin-dependent processes by imaging of living cells.In a third study, encouraged by the result with citrine-profilin I, a similar approach was taken to generate a fluorescent variant of the profilin isoform IIa with the mKate2 protein as the fused fluorophore. The cellular localisation of this profilin-constuct revealed an association with focal adhesions. Further analysis after introduction of a mutation in the profilin-moiety that distorts poly-proline binding, clearly showed that the distribution to focal adhesions depends on the binding to poly-proline, pointing to a small group of candidate proteins as recruiting molecules. It was not possible to detect a localization to microtubules suggesting that this interaction is unique to profilin I. This means that the data in my thesis also point to distinct functions for the two major mammalian profilin isoforms; profilin I being connected to microtubule organization in addition to its classical role as an actin partner, and profilin IIa-function may be more closely linked to focal adhesion and cell-substratum attachments.
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| 10. |
- Nejedlá, Michaela, et al.
(författare)
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The actin regulator profilin 1 is functionally associated with the mammalian centrosome
- 2021
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Ingår i: Life Science Alliance. - : Life Science Alliance, LLC. - 2575-1077. ; 4:1
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Tidskriftsartikel (refereegranskat)abstract
- Profilin 1 is a crucial actin regulator, interacting with monomeric actin and several actin-binding proteins controlling actin polymerization. Recently, it has become evident that this profilin isoform associates with microtubules via formins and interferes with microtubule elongation at the cell periphery. Recruitment of microtubule-associated profilin upon extensive actin polymerizations, for example, at the cell edge, enhances microtubule growth, indicating that profilin contributes to the coordination of actin and microtubule organization. Here, we provide further evidence for the profilin-microtubule connection by demonstrating that it also functions in centrosomes where it impacts on microtubule nucleation.
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