| 1. |
- Bosa Ochoa, Carlos Felipe, et al.
(författare)
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Respuesta de Tecia solanivora (Lepidoptera: Gelechiidae) a compuestos volátiles de papa, Solanum tuberosum
- 2011
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Ingår i: Revista Colombiana De Entomologia. - 0120-0488. ; 37, s. 1-7
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Tidskriftsartikel (refereegranskat)abstract
- Trampas cebadas con volátiles de plantas como fuente de atrayente pueden ser una herramienta importante para el monitoreo o control de insectos plaga. La polilla guatemalteca,Teciasolanivora, es una plaga limitante en el cultivo de la papa y aún se desconoce su relación con su planta hospedera, la papa. Se estudió el efecto que producen olores de las diferentes estructuras de la planta hospedera en el comportamiento del insecto. También se estudiaron los compuestos metilfenilacetato y sulcatón, dos compuestos volátiles de la planta identificados previamente que son emitidos por las flores y los tubérculos, además de producir respuestas antenales de T.solanivora. Se realizaron ensayos en olfatómetro y en una jaula recubierta con tul con la utilización de trampas de captura. En olfatómetro la estructura que más atrajo a las hembras fue la flor. En la jaula se registró una mayor captura de hembras en trampas cebadas con el compuesto metilfenilacetato a 100μg, y no se observó un efecto sinérgico al combinar los dos compuestos. Los resultados sugieren la evaluación de otros compuestos sintéticos de la planta de papa y sus mezclas para profundizar en el comportamiento de este insecto.
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| 2. |
- Dejene Biasazin, Tibebe, et al.
(författare)
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Detection of Volatile Constituents from Food Lures by Tephritid Fruit Flies
- 2018
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Ingår i: Insects. - : MDPI AG. - 2075-4450. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Tephritid fruit flies require protein for sexual and gonotrophic development. Food-based lures are therefore widely used in strategies to detect and control fruit flies in the Tephritidae family. However, these baits are attractive to a broad range of insect species. We therefore sought to identify volatiles detected by the fly antennae, with the goal to compose lures that more specifically target tephritids. Using gas chromatography-coupled electroantennographic detection (GC-EAD) we screened for antennal responses of four important tephritid species to volatile compounds from five commercially available protein-based baits. Antennal active compounds were reconstituted in synthetic blends for each species and used in behavioral assays. These species-based blends were attractive in olfactometer experiments, as was a blend composed of all antennally active compounds from all the four species we observed (tested only in Bactrocera dorsalis, Hendel). Pilot field tests indicate that the blends need to be further evaluated and optimized under field conditions.
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| 3. |
- Dejene Biasazin, Tibebe, et al.
(författare)
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Identification of Host Blends that Attract the African Invasive Fruit Fly, Bactrocera invadens
- 2014
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Ingår i: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 40, s. 966-976
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Tidskriftsartikel (refereegranskat)abstract
- Bactrocera invadens, an invasive fruit fly species in the Afro-tropical region belonging to the Bactrocera dorsalis complex, causes considerable damage to fruit production and productivity. We sought to find attractants from hosts of B. invadens that could serve as baits in traps for monitoring and management of this pest. The attractiveness of volatiles from four different fruit species (mango, guava, banana and orange) at two stages of ripeness (ripe or unripe) was tested in an olfactometer assay. All fruits were attractive against a clean air control. Using hexane extracts of volatile collections of fruits, we demonstrated that male flies preferred the volatiles of ripe guava and orange over unripe fruit extracts. There was a slight difference in preference between females and males; females preferred orange to guava and mango, whereas males preferred mango and guava to orange. Gas chromatography/electroantennographic detection (GC/EAD) and GC/mass spectrometry (GC/MS) were used to identify compounds to which B. invadens antennae were sensitive. GC/EAD recordings from distal and medio-central parts of the fly antenna showed responses to a number of compounds from each fruit species, with esters dominating the responses. Synthetic blends were made for each fruit species using the shared antennally active compounds in ratios found in the extracts. In the olfactometer, B. invadens was most attracted to the banana and orange blends, followed by the mango and guava blends. The synthetic banana blend was as attractive as the volatile collection of banana, although both were less attractive than the fruit. The results demonstrate that composing attractive blends from GC/EAD-active constituents shared by host fruits can be effective for formulating attractive synthetic host mimics for generalist fruit fly species, such as B. invadens.
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| 4. |
- Karlsson, Miriam Frida
(författare)
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Effect of fruit and host fly species on the associative learning by Fopius arisanus
- 2019
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Ingår i: Bulletin of Entomological Research. - 0007-4853 .- 1475-2670. ; 109, s. 649-658
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Tidskriftsartikel (refereegranskat)abstract
- Parasitoids, released in augmentative biological control programmes, which display a rapid host-location capacity, have a higher likelihood of successfully controlling target pest species. By learning to associate sensory cues to a suitable oviposition site, might parasitoids used as biological control agents, locate hosts more rapidly, and perhaps increase the efficacity of e.g. Tephritidae fruit fly management. We studied associative learning of Fopius arisanus (Hymenoptera: Braconidae) and tested its range of learning in natural and conditional hosts and host fruits, i.e. Bactrocera dorsalis, Zeugodacus cucurbitae, Ceratitis capitata and Ceratitis cosyra (Diptera: Tephritidae) and on fruits (papaya, tomato, banana). Naive female F. arisanus were compared with experienced wasps, which had been offered infested and non-infested fruit, and been allowed to oviposit. Preferences for olfactory cues from infested fruits were thereafter assessed in a two-choice olfactometer. Naive and trained parasitoids preference differed in general and non-responders to infested fruits were higher among naive parasitoids. The trained wasps preferred the fruit infested in the training more than the control fruit, for all combination, except when C. cosyra infested the fruits, hence avoidance behavioural response was observed towards the odour of the infested fruit. Fopius arisanus was capable of behaviourally respond to the learned information, e.g. associative odour learning was achieved, yet limited depending on interaction level, fruit fly and fruit combination. To create F. arisanus preference of an associated odour, it might hence be needed to ensure oviposition in perceived suitable host and host fruit, for the parasitoid learning to become favourable in a biological control setup.
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| 5. |
- Karlsson, Miriam Frida, et al.
(författare)
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Efficacy of a postharvest treatment aiming at eradication of all developmental stages of Tecia solanivora in ware potatoes
- 2023
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Ingår i: EFSA Journal. - : Wiley. - 1831-4732. ; 21
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Tidskriftsartikel (refereegranskat)abstract
- The European Commission requested the EFSA Panel on Plant Health to prepare and deliver a scientific opinion on the efficacy of a postharvest treatment aiming to eradicate all developmental stages of Guatemalan potato tuber moth Tecia solanivora (Lepidoptera: Gelechiidae) in ware potatoes. The Panel evaluated the scientific publication describing the elevated CO2 treatment, which was defined as: 10-day exposure to 30% CO2, 20% O-2 and 50% N-2 in controlled atmosphere at 17 degrees C on the variety Negra Yema de Huevo (Papas Antiguas de Canarias, PDO potatoes, Solanum chaucha). In the scientific publication, the treatment was applied under semi-commercial and commercial conditions on artificially and field-infested tubers. The effect of the pest developmental stage on the treatment efficacy was investigated with artificial infestation of potato tubers with eggs, neonate and second instar larvae. Pupae and adults were placed in separate containers during the treatment. However, the third and fourth larval instars were not investigated. Further limitations were the sample size in the experiments, the mortality rate in the control group and the unknown level of infestation of the naturally infested potato tubers. It was not possible to evaluate the degree of pest freedom due to incomplete data on the conditions of production, i.e. the infestation level in the field. The Panel was able to conclude that although no surviving insects were observed in the performed experiments, the statistical evaluation of the presented results from the commercial trial indicate that it cannot be excluded that insects would survive the treatment. For example, based on the data provided the 95% confidence interval of the survival rate for eggs was: 0%-0.453%.
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| 6. |
- Karlsson, Miriam Frida
(författare)
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Exotic or Native? Interspecific Competition in the Parasitization of the Fruit Fly Ceratitis cosyra
- 2017
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Konferensbidrag (refereegranskat)abstract
- Fopius arisanus Sonan and Fopius caudatus Szèpligeti (Hymenoptera: Braconidae) are two solitary endoparasitoids of tephritid fruit flies that parasitize eggs of fruit flies infesting the same fruits. We studied intraspecific competition between these two parasitoids species in the mango fruit fly Ceratitis cosyra (Walker) (Diptera: Tephritidae). Based on previous documentation of the equal chance of survival between F. arisanus and Fopius ceratititvorus Wharton (Hymenoptera: Braconidae) while co-parasitizing (Bokonon-Ganta et al., 2005), we hypothesized that we would not find a superior competitor between the two parasitoids.
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| 7. |
- Karlsson, Miriam Frida
(författare)
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First report of Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) in the Republic of Benin
- 2018
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Ingår i: BioInvasions Records. - : Regional Euro-Asian Biological Invasions Centre Oy (REABIC). - 2242-1300. ; 7, s. 463-468
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Tidskriftsartikel (refereegranskat)abstract
- Tomato leafminer Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is a serious pest of tomato Solanum lycopersicum L. (Solanaceae) originating from South America. Due to previous outbreaks in neighbouring West African countries, surveillance was ongoing since 2014 in Benin. Tomato plants were regularly inspected and pheromone traps placed in tomato-producing localities in northern, southern and central Benin, including fields close to the border with Niger and Nigeria. In late 2017 and beginning of 2018 Gelechiidae moths were obtained from both the traps and the reared plant material. Morphological examination and molecular analysis of the gained specimens allowed to confirm the presence of the pest in the northern and central regions of Benin. We hence confirm the presence of T. absoluta in yet another West African country.
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| 8. |
- Karlsson, Miriam Frida, et al.
(författare)
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Guatemalan potato moth Tecia solanivora distinguish odour profiles from qualitatively, different potatoes Solanum tuberosum L.
- 2013
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Ingår i: Phytochemistry. - : Elsevier BV. - 0031-9422 .- 1873-3700. ; 85, s. 72-81
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Tidskriftsartikel (refereegranskat)abstract
- Guatemalan potato moth, Tecia solanivora, lay eggs in the soil nearby potato Solanum spp. and larvae feed on the tubers. We investigated the oviposition behaviour of T. solanivora females and the survival of larval offspring on healthy vs. stressed, i.e. light exposed and/or damaged potato tubers. In choice tests, females laid significantly more eggs in response to potato odour of healthy tubers and female oviposition preference correlated with higher larval survival. Survival of larvae was negatively correlated with the tuber content of the steroid glycoalkaloids alpha-solanine and alpha-chaconine: healthy potatoes contained lower amounts than stressed tubers, ranging from 25 to 500 mu g g(-1) and from 30 to 600 mu g g(-1), respectively. Analysis of volatile compounds emitted by potato tubers revealed that stressed tubers could clearly be distinguished from healthy tubers by the composition of their volatile profiles. Compounds that contributed to this difference were e.g. decanal, nonanal, isopropyl myristate, phenylacetaldehyde, benzothiazole, heptadecane, octadecane, myristicin, E,E-alpha-famesene and verbenone. Oviposition assays, when female moths were not in contact with the tubers, clearly demonstrated that volatiles guide the females to lay fewer eggs on stressed tubers that are of inferior quality for the larvae. We propose that volatiles, such as sesquiterpenes and aldehydes, mediate oviposition behaviour and are correlated with biosynthetically related, non-volatile compounds, such as steroidal glycoalkaloids, which influence larval survival. We conclude that the oviposition response and larval survival of T. solanivora on healthy vs. stressed tubers supports the preference performance hypothesis for insect herbivores. (C) 2012 Elsevier Ltd. All rights reserved.
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| 9. |
- Karlsson, Miriam Frida
(författare)
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Habitat factors associated with Fopius caudatus parasitism and population level of its host, Ceratitis cosyra
- 2020
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Ingår i: Entomologia Experimentalis et Applicata. - : Wiley. - 0013-8703 .- 1570-7458. ; 168, s. 28-40
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Tidskriftsartikel (refereegranskat)abstract
- Biotic and abiotic factors affect herbivores and their natural enemies and understanding of their requirements may permit habitat modification enabling conservation biological control. Ceratitis cosyra Walker (Diptera: Tephritidae), an African-native fruit fly pest is mostly parasitized by the parasitoid wasp Fopius caudatus Szepligeti (Hymenoptera: Braconidae). To assess F. caudatus habitat, the wasp parasitism levels and infestation of its fruit fly host were examined in Sarcocephalus latifolius (Smith) Bruce (Rubiaceae), a shrub of which the fruits are among the preferred hosts of C. cosyra and F. caudatus. Fruit-collection site descriptions, including plant species presence, were analysed in relation to the target insect abundances (emergence from target fruit). Ceratitis cosyra and F. caudatus emerged from all sites; nonetheless, their population levels were associated with both abiotic and biotic factors, of which some can be manipulated. Several factors, such as cultivation level, topography, and vegetation coverage, were correlated with F. caudatus parasitism. Ceratitis cosyra infestation level was correlated with factors such as density of S. latifolius, vegetation cover, cultivation practices, temperature, altitude, rainfall pattern, and stoniness. Proximity to other fruit fly host plants correlated with both pest abundance and F. caudatus parasitism level of the fruit fly. The findings that insects' interactions and abundance are influenced by habitat structure and that parasitism is positively related to natural habitat indicates the importance of maintaining natural habitats in closeness to cultivated areas with the aim of enhancing pest suppression by parasitoids. Further studies should attempt to identify how plant species composition in and around orchards could affect the management of tephritid fruit fly pests.
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| 10. |
- Karlsson, Miriam Frida, et al.
(författare)
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Host-plant location by the Guatemalan potato moth Tecia solanivora is assisted by floral volatiles
- 2017
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Ingår i: Chemoecology. - : Springer Science and Business Media LLC. - 0937-7409 .- 1423-0445. ; 27, s. 187-198
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Tidskriftsartikel (refereegranskat)abstract
- Insects locate their host plants using mainly visual and olfactory cues, generally of the exploited plant structure. However, when the resource is difficult to access, it could be beneficial to utilise indirect cues, which indicates the presence of reward (e.g., oviposition site or mate). In the present study, we investigated the host-plant location strategy of the monophagous Guatemalan potato moth Tecia solanivora (Lepidoptera: Gelechiidae). The larva of the moth feed exclusively on potato Solanum spp. (Solanaceae) tubers usually hidden below ground. Using electrophysiological and behavioural tests, we characterised the olfactory cues mediating the attraction of the moth towards their host plant. Odour blends were made to represent different potato structures: tubers, foliage, and flowers. Synthetic blends were created by combining potato-emitted compounds that were antennal active which showed positive dose-response. Attraction to these blends of compounds in relation to the mating status of males and females was tested in dual-choice Y-tube assays. Both males and females, virgin and mated, were attracted to a three-compound blend representing flower odour, while foliage and tuber blends attracted neither sexes. Oviposition bioassays indicated additionally that the floral blend enhances oviposition. We show that potato flower odour might indicate the presence of an oviposition site for the female and possibly an increased mating opportunity for both sexes. Our results provide one of the few examples of the use of floral odour as a reliable indicator of host and probably mating possibility for phytophagous insects exploiting a site spatially separated from the flower.
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