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- Zhang, Y. S., et al.
(författare)
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Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors
- 2017
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 114:12, s. E2293-E2302
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Tidskriftsartikel (refereegranskat)abstract
- Organ-on-a-chip systems areminiaturizedmicrofluidic 3D human tissue and organ models designed to recapitulate the important biological and physiological parameters of their in vivo counterparts. They have recently emerged as a viable platform for personalized medicine and drug screening. These in vitro models, featuring biomimetic compositions, architectures, and functions, are expected to replace the conventional planar, static cell cultures and bridge the gap between the currently used preclinical animal models and the human body. Multiple organoid models may be further connected together through the microfluidics in a similar manner in which they are arranged in vivo, providing the capability to analyze multiorgan interactions. Although a wide variety of human organ-on-a-chip models have been created, there are limited efforts on the integration of multisensor systems. However, in situ continual measuring is critical in precise assessment of the microenvironment parameters and the dynamic responses of the organs to pharmaceutical compounds over extended periods of time. In addition, automated and noninvasive capability is strongly desired for long-term monitoring. Here, we report a fully integrated modular physical, biochemical, and optical sensing platform through a fluidics-routing breadboard, which operates organ-on-a-chip units in a continual, dynamic, and automated manner.We believe that this platform technology has paved a potential avenue to promote the performance of current organ-on-a-chip models in drug screening by integrating a multitude of real-time sensors to achieve automated in situ monitoring of biophysical and biochemical parameters.
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| 2. |
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| 3. |
- Pelaz, B, et al.
(författare)
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Diverse Applications of Nanomedicine
- 2017
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Ingår i: ACS nano. - : American Chemical Society (ACS). - 1936-086X .- 1936-0851. ; 11:3, s. 2313-2381
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Tidskriftsartikel (refereegranskat)
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| 4. |
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| 5. |
- Shaegh, S. A. M., et al.
(författare)
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A microfluidic optical platform for real-time monitoring of pH and oxygen in microfluidic bioreactors and organ-on-chip devices
- 2016
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Ingår i: Biomicrofluidics. - : American Institute of Physics (AIP). - 1932-1058. ; 10:4
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Tidskriftsartikel (refereegranskat)abstract
- There is a growing interest to develop microfluidic bioreactors and organ-on-chipplatforms with integrated sensors to monitor their physicochemical properties and tomaintain a well-controlled microenvironment for cultured organoids. Conventionalsensing devices cannot be easily integrated with microfluidic organ-on-chip systemswith low-volume bioreactors for continual monitoring. This paper reports on thedevelopment of a multi-analyte optical sensing module for dynamic measurementsof pH and dissolved oxygen levels in the culture medium. The sensing system wasconstructed using low-cost electro-optics including light-emitting diodes and siliconphotodiodes. The sensing module includes an optically transparent window formeasuring light intensity, and the module could be connected directly to a perfusionbioreactor without any specific modifications to the microfluidic device design. Acompact, user-friendly, and low-cost electronic interface was developed to controlthe optical transducer and signal acquisition from photodiodes. The platformenabled convenient integration of the optical sensing module with a microfluidicbioreactor. Human dermal fibroblasts were cultivated in the bioreactor, and thevalues of pH and dissolved oxygen levels in the flowing culture medium were measuredcontinuously for up to 3 days. Our integrated microfluidic system providesa new analytical platform with ease of fabrication and operation, which can beadapted for applications in various microfluidic cell culture and organ-on-chipdevices.
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| 9. |
- Lindström, Sara, et al.
(författare)
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Nanoporous titania coating of microwell chips for stem cell culture and analysis
- 2010
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Ingår i: Journal of Biomechanical Science and Engineering. - : Japan Society of Mechanical Engineers. - 1880-9863. ; 5:3, s. 272-279
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Tidskriftsartikel (refereegranskat)abstract
- Stem cell research is today an active and promising field of research. To learn more about the biology of stem cells, technical improvements are needed such as tools to study stem cells in order to characterize them further and to gain insights to the molecular regulations of their maintenance, differentiation and identification. Common procedure when studying stem cells is to coat the surface where the stem cells are to be cultured with organic materials like matri-gel, poly-L-lysine and fibronectin. The resulting coating is usually relatively fragile and it is difficult to know if the coating is evenly distributed. In addition, these forms of coatings cannot be sterilized and re-used, but must be added as an initial, time-consuming step in the daily protocol. A microwell chip with hundreds of 500 nl wells has recently been shown to be a useful tool for stem cell culturing. This platform is here modified to facilitate and improve the coating conditions for adherent cell culture. A robust and highly porous film of TiO2 is deposited in the wells prior cell seeding. TiO2 is known to be biocompatible and provides a surface that is even and well characterized, simple to produce and re-usable. Furthermore it enables the microwell chips to be stored pre-coated for longer periods of time before use. We investigated the growth of rat mesenchymal stem cells on nanoporous titania films and found that they proliferated much faster than on conventional coatings. The combination of the robust TiO2 coating of the microwell chip enables thousands of individually separated single, or clones of, stem cells to be studied simultaneously and opens up the possibility for more user-friendly cell culturing.
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| 10. |
- Luo, Yifei, et al.
(författare)
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Technology Roadmap for Flexible Sensors
- 2023
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Ingår i: ACS Nano. - : American Chemical Society. - 1936-0851 .- 1936-086X. ; 17:6, s. 5211-5295
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Forskningsöversikt (refereegranskat)abstract
- Humans rely increasingly on sensors to address grand challenges and to improve quality of life in the era of digitalization and big data. For ubiquitous sensing, flexible sensors are developed to overcome the limitations of conventional rigid counterparts. Despite rapid advancement in bench-side research over the last decade, the market adoption of flexible sensors remains limited. To ease and to expedite their deployment, here, we identify bottlenecks hindering the maturation of flexible sensors and propose promising solutions. We first analyze challenges in achieving satisfactory sensing performance for real-world applications and then summarize issues in compatible sensor-biology interfaces, followed by brief discussions on powering and connecting sensor networks. Issues en route to commercialization and for sustainable growth of the sector are also analyzed, highlighting environmental concerns and emphasizing nontechnical issues such as business, regulatory, and ethical considerations. Additionally, we look at future intelligent flexible sensors. In proposing a comprehensive roadmap, we hope to steer research efforts towards common goals and to guide coordinated development strategies from disparate communities. Through such collaborative efforts, scientific breakthroughs can be made sooner and capitalized for the betterment of humanity.
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