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Sökning: WFRF:(Kim JinHo 1984)

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1.
  • Choi, BoHyun, 1986, et al. (författare)
  • Redesign and reconstruction of a mevalonate pathway and its application in terpene production in Escherichia coli
  • 2019
  • Ingår i: Bioresource Technology Reports. - : Elsevier BV. - 2589-014X. ; 7
  • Tidskriftsartikel (refereegranskat)abstract
    • Metabolic engineering of heterologous biosynthetic pathways in microbial hosts is an alternative way for producing high-value biochemicals. Here, heterologous mevalonate (MVA) and prenyl pyrophosphate (IPP) pathways of a new isolate Enterococcus kingsejongensis Gf1 were redesigned and reconstructed in Escherichia coli. Two MVA upstream enzymes of E. kingsejongensis Gf1 were functionally expressed and overexpression of the two enzymes led to a yield of 52.5 g/L MVA after 65 h of fed-batch fermentation. Next, the functions of three MVA downstream enzymes were verified by addition of exogenous MVA. Finally, whole MVA pathway enzymes and IPP pathway enzymes were combined in E. coli in order to overproduce squalene and β-carotene. The E. coli strains coexpressing entire MVA and IPP pathway enzymes enhanced squalene production by 15-fold and β-carotene production by 2.5-fold.
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2.
  • Mokkapati, Venkata Raghavendra Subrahmanya Sar, 1981, et al. (författare)
  • Bacterial response to graphene oxide and reduced graphene oxide integrated in agar plates
  • 2018
  • Ingår i: Royal Society Open Science. - : The Royal Society. - 2054-5703. ; 5:11
  • Tidskriftsartikel (refereegranskat)abstract
    • There are contradictory reports in the literature regarding the anti-bacterial activity of graphene, graphene oxide (GO) and reduced graphene oxide (rGO). This controversy is mostly due to variations in key parameters of the reported experiments, like: type of substrate, form of graphene, number of layers, type of solvent and most importantly, type of bacteria. Here, we present experimental data related to bacterial response to GO and rGO integrated in solid agar-based nutrient plates-a standard set-up for bacterial growth that is widely used by microbiologists. Bacillus subtilis and Pseudomonas aeruginosa strains were used for testing bacterial growth. We observed that plate-integrated rGO showed strong anti-bacterial activity against both bacterial species. By contrast, plate-integrated GO was harmless to both bacteria. These results reinforce the notion that the response of bacteria depends critically on the type of graphene material used and can vary dramatically from one bacterial strain to another, depending on bacterial physiology.
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