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- Kumar, S. Anil, et al.
(författare)
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Beyond just being foot soldiers : osmotin like protein ( OLP ) and chitinase ( Chi11 ) genes act as sentinels to confront salt, drought, and fungal stress tolerance in tomato
- 2016
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Ingår i: Environmental and Experimental Botany. - : Elsevier. - 0098-8472 .- 1873-7307. ; 132, s. 53-65
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Tidskriftsartikel (refereegranskat)abstract
- Osmotin like protein (OLP) and chitinase (Chi11) belong to pathogenesis-related (PR) class of proteins and are induced during both biotic and abiotic stresses. Transgenic tomato was developed with OLP and Chi11 via in vitro and in planta transformation methods. Transgenes integration and transcript levels were confirmed by multiplex PCR, DNA blot, and multiplex reverse transcriptase PCR. Homozygous T2 transgenics when evaluated for salt, drought, and fungal stresses showed enhanced tolerance compared to untransformed controls (UC). Transgenics showed enhanced endochitinase activity and root biomass under normal conditions. Transformants also displayed higher proline content, K+, relative water content, chlorophyll fluorescence, total biomass, vascular conductivity, and fruit yield than the UC under stress conditions. Co-immunoprecipitation revealed that Chi11 co-expresses with phosphofructokinase2 (PFK2), which may play a role in enhanced root biomass. qPCR analysis resulted in higher transcript levels of OLP, Chi11, and PFK2 in transgenics as compared to the untransformed controls. Our findings suggest the use of multiples genes to confer multiple stress tolerance for enhanced crop productivity. This work unveils a new molecular player PFK2, which may play a role in enhanced root biomass.
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| 2. |
- Rampuria, Sakshi, et al.
(författare)
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Pathogen-induced AdDjSKI of the wild peanut, Arachisdiogoi, potentiates tolerance of multiple stresses in E. coli andtobacco
- 2018
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Ingår i: Plant Science. - : Elsevier BV. - 0168-9452. ; 272, s. 62-74
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Tidskriftsartikel (refereegranskat)abstract
- A gene encoding a serine-rich DnaJIII protein called AdDjSKI that has a 4Fe-4S cluster domain was found to be differentially upregulated in the wild peanut, Arachis diogoi in its resistance responses against the late leaf spot causing fungal pathogen Phaeoisariopsis personata when compared with the cultivated peanut, Arachis hypogaea. AdDjSKI is induced in multiple stress conditions in A. diogoi. Recombinant E. coli cells expressing AdDjSKI showed better growth kinetics when compared with vector control cells under salinity, osmotic, acidic and alkaline stress conditions. Overexpression of this type three J-protein potentiates not only abiotic stress tolerance in Nicotiana tabacum var. Samsun, but also enhances its disease resistance against the phytopathogenic fungi Phytophthora parasitica pv nicotianae and Sclerotinia sclerotiorum. In the present study we show transcriptional upregulation of APX, Mn-SOD and HSP70 under heat stress and increased transcripts of PR genes in response to fungal infection. This transmembrane-domain-containing J protein displays punctate localization in chloroplasts. AdDjSKI appears to ensure proper folding of proteins associated with the photosynthetic machinery under stress.
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