| 1. |
- Jablonowska, Barbara, 1948-, et al.
(författare)
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Blocking antibodies in blood from patients with recurrent spontaneous abortion in relation to pregnancy outcome and intravenous immunoglobulin treatment
- 2001
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Ingår i: American Journal of Reproductive Immunology. - : Wiley. - 1046-7408 .- 1600-0897 .- 8755-8920. ; 45:4, s. 226-231
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Tidskriftsartikel (refereegranskat)abstract
- PROBLEM: To study whether the occurrence of mixed lymphocyte culture (MLC) blocking antibodies is associated with pregnancy outcome in women with unexplained recurrent spontaneous abortion (RSA) and the in vivo effect of intravenous immunoglobulin (IVIG) treatment on MLC blocking effect.METHOD OF STUDY: Blood samples from 41 RSA patients were obtained before and after pregnancy, and blocking antibodies were estimated by one-way MLC assay. The patients received IVIG or placebo (saline) during pregnancy. Additionally, pre-pregnancy blood samples from 31 RSA women and 10 controls were obtained.RESULTS: We found no correlation between blocking antibodies before pregnancy and the pregnancy outcome. The occurrence of blocking antibodies was not affected by pregnancy or IVIG treatment.CONCLUSIONS: Blocking antibodies have no predictive value for the pregnancy outcome in RSA patients, and their production seems not to be affected by IVIG.
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| 2. |
- Jablonowska, Barbara, 1948-, et al.
(författare)
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Prevention of recurrent spontaneous abortion by intravenous immunoglobulin : a double-blind placebo-controlled study
- 1999
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Ingår i: Human Reproduction. - : Oxford University Press (OUP). - 0268-1161 .- 1460-2350. ; 14:3, s. 838-841
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to evaluate the therapeutic efficacy of intravenous immunoglobulin (IVIG) in the prevention of recurrent spontaneous abortion (RSA). In a double-blind, randomized, placebo-controlled study, 41 women with a history of unexplained recurrent spontaneous abortion were treated with IVIG or saline infusions during pregnancy. The birth of a child was considered a successful outcome. The overall success rate was 77% in the IVIG group compared with 79% in the placebo group. For women with primary RSA the success rates were 82 (IVIG) and 89% (placebo), and for women with secondary RSA the rates were 73 (IVIG) and 70% (placebo). We found no statistically significant difference in treatment results between IVIG and placebo.
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| 3. |
- Jablonowska, Barbara, 1948-, et al.
(författare)
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T and B lymphocyte subsets in patients with unexplained recurrent spontaneous abortion : IVIG versus placebo treatment
- 2002
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Ingår i: American Journal of Reproductive Immunology. - : Wiley. - 1046-7408 .- 1600-0897. ; 48:5, s. 312-318
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Tidskriftsartikel (refereegranskat)abstract
- Jablonowska B, Palfi M, Matthiesen L, Selbing A, Kjellberg S, Ernerudh J. T and B Lymphocyte subsets in patients with unexplained recurrent spontaneous abortion: IVIG versus placebo treatment. AJRI 2002; 48:312–318 © Blackwell Munksgaard, 2002PROBLEM: To investigate circulating lymphocyte subsets in women with recurrent spontaneous abortion (RSA) in relation to pregnancy outcome and to treatment with intravenous immunoglobulin (IVIG).METHOD OF STUDY: Forty-one women with a history of unexplained RSA were examined during first trimester of pregnancy before IVIG or placebo treatment and after pregnancy. The results were compared with five healthy, non-pregnant women and five women in the first trimester of normal pregnancy. Circulating lymphocyte subsets with focus on T-cell subpopulations were determined by flow cytometry.RESULTS: The proportions of human leukocyte antigen (HLA)-DR positive T cells (CD3+ HLA-DR+), T-killer/effector cells (CD8+ S6F1+) and B cells (CD19+) were increased, whereas the proportion of T-suppressor/inducer cells (CD4+ CD45RA+) was decreased during first trimester pregnancy of RSA women compared with pregnant normal controls. T and B lymphocyte subsets did not correlate with pregnancy outcome on either IVIG or placebo group.CONCLUSIONS: In RSA patients, the immune system seems to be activated in contrast to the suppression noted in normal pregnancy.
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| 4. |
- Kjellberg, Svante, 1946-, et al.
(författare)
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Knowledge of and attitudes towards infertility held by members of two county councils in Sweden
- 2000
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Ingår i: Acta Obstetricia et Gynecologica Scandinavica. - : Wiley. - 0001-6349 .- 1600-0412. ; 79:11, s. 1015-1020
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Tidskriftsartikel (refereegranskat)abstract
- Background. To investigate Swedish county council members knowledge of the laws regulating infertility treatment, their understanding of the suggestions put forward in the report 'Priorities in Health Care' and their attitudes towards infertility. Methods. All members (n= 182) of the 1998 county councils of Linkoping and Jonkoping were asked to participate in the study. In total, 153 answered the questionnaire. Results. Different age groups did not show different levels of knowledge. Similarly, there was no significant difference between men and women concerning their knowledge of infertility laws. Furthermore, those committee members who were very positive towards infertility treatment did not have any better knowledge of such treatment than those who were less positive. The men and women who themselves had previously had or were dealing with infertility problems at the time of the survey (12 per cent) displayed the same level of knowledge as the other council members. They also expressed similar attitudes. The members showed very little familiarity with the priority groups suggested in the report from the Health Care Priority Committee. For example, only 18 per cent knew that this committee in priority group III placed infertility treatment and investigation. 31.3 per cent answered that infertility was placed in group V, a group that does not exist. The majority stated that they had a positive attitude towards the treatment of infertility. This is indicated, for example, by their rejection of the statement 'it's [infertility is] a luxury problem'. Conclusions. The relative lack of knowledge of Swedish law and of the report from the Health Care Priority Committee might lead to a lower level of commitment to dealing with the problems that infertile couples face than if the members were better informed. In other words, this lack of knowledge might influence the decisions of the county council members.
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| 5. |
- Kjellberg, Svante, 1946-
(författare)
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Zinc and human sperm chromatin
- 1993
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- X-rny microanalysis, Sephadex® chromatography of seminal plasma and exposure of spermatozoa to sodium dodecyl sulphate (SDS), were methods used to study the zinc content of sperm chromatin, the nature of the zinc binding ligands present in seminal plasma and the stability of the sperm chromatin in whole semen samples from fertile and infertile men. Split ejaculates were used to study the same variables in different fractions of the same ejaculate.To obtain "pure" samples of prostatic fluid ejaculates were collected from four men with agenesis of the Wolffian ducts.Spermatozoa were obtained from the vas deferens and the ejaculates from each of four men operated on with vasectomy to study how contact between the sperm and seminal plasma might influence the eventual content of zinc in the sperm chromatin.The relative content of zinc in sperm chromatin was extremely low in the vasal spermatozoa as compared to the content of zinc in the chromatin of the ejaculated spermatozoa. This indicates that sperm may take up zinc from the seminal plasma. Another role for zinc in seminal fluid could be to prevent loss of zinc from the spenn chromatin of ejaculated spermatozoa.The proportion of sperm heads which remained stable after exposure to SDS was found to be related to the relative content of zinc in the sperm chromatin. The content of zinc in spermatozoa from fertile men corresponded to one zinc atom per protamine molecule in the chromatin.The relative content of zinc in the sperm chromatin had no simple relation to the zinc concentration in the seminal plasma. It was, however, found that men with a relative low content of zinc in the sperm chromatin had a disturbed sequence of ejaculation. They ejaculate the majority ofspennatozoa in an ejaculate fraction with a high admixture of seminal vesicular fluid. These fractions had a low zinc/fructose molar ratio i e a low ratio between prostatic derived zinc and vesicular derived fructose. Furthermore in these fractions seminal plasma zinc was to a high degree OOund to high molecular weight ligands (Zn~LnMw).The low molecular weight zinc binding ligand (Zn-LLMW) was shown to be of prostatic origin and identical to citrate.Sperm samples derived from fertile semen donors (n=5) a had a' higher proportion of sperm heads remaining stable after exposure to SDS, as well as a higher content of zinc in the sperm chromatin as compared to samples from group of men with "unexplained" infertility (n=lO). A larger sample of men investigated because of infertility (n=ll5) was also studied. Onequarter of these men had an impaired sperm chromatin stability with less than 80 per cent stable spermatozoa. The proportion zinc bound to the ligand Zn-LIIMW varied between 2~67 per cent. It is concluded that the liquefied seminal plasma, despite a high zinc concentration, could act as a zinc~ chelating medium that could deprive spermatozoa of zinc. It was, however, not possible to predict the individual fecundity in this group of infertile men by measuring the proportion of sperm heads, which remained stable after exposure to SDS or the proportion of zinc in the seminal fluid which was bound to Zn~L'IMW. The first split~ejaculate fraction should probably be studied instead of the whole mixed ejaculate to evaluate whether spermatozoa that tentatively enter the cervical mucus have an appropriate zinc~dependent stabilisation of their chromatin.
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| 6. |
- Leeb-Lundberg, Sara, et al.
(författare)
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Helping parents to tell their children about the use of donor insemination (DI) and determining their opinions about open-identity sperm donors
- 2006
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Ingår i: Acta Obstetricia et Gynecologica Scandinavica. - : Wiley. - 0001-6349 .- 1600-0412. ; 85:1, s. 78-81
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Tidskriftsartikel (refereegranskat)abstract
- Objective. To look at the level of compliance with Swedish law whether or not parents intend to tell their child about donor insemination. We also wanted to look at the parents' attitudes towards open-identity sperm donors and at relationships within the family. Method. All parents who were treated and gave birth to a child through donor insemination from 1997 to 2003 were included in the study. Sixteen of 20 couples (80%) were willing to take part in an interview, where the men and women were interviewed separately. The children of these couples had an average age of 2.9 years. Results. Three of the 16 couples had told their child about donor insemination and 9 couples intended to tell the child when he/she was older. Thus 12 couples (75%) had disclosed or planned to inform their child in the future. Fourteen of 16 couples had told others about the donor insemination. The majority (20 of 31 individuals) had a positive attitude towards open-identity for sperm donors and 21 of 31 would have chosen an open-identity sperm donor if they had had the choice between that and an anonymous donor. All the parents felt they had an equal relationship with their child. Conclusion. Couples who conceived a child through donor insemination are open about the donor insemination, both to other people in their surroundings and in their intention to tell the child. These families seem to be functioning well with relaxed attitudes towards the donor insemination process. © 2006 Taylor & Francis.
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| 7. |
- Sydsjö, Gunilla, 1956-, et al.
(författare)
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Relationships and parenthood in couples after assisted reproduction and in spontaneous primiparous couples : A prospective long-term follow-up study
- 2002
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Ingår i: Human Reproduction. - 0268-1161 .- 1460-2350. ; 17:12, s. 3242-3250
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Tidskriftsartikel (refereegranskat)abstract
- Background: The aim of this study was to analyse relationships and parenthood in primiparous IVF couples and spontaneous primiparous couples. Method: In total, 110 consecutive IVF couples were studied. The control group was matched for women's age and selected out of the total spontaneous pregnant population in the study area. Questionnaires and semi-structured telephone interviews were used to gain information about sociodemographic data, couples' relationships, and the children's health, temperament and behaviour. Obstetrical variables concerning the course and outcome of pregnancy and delivery as well as the health status of the new-borns were obtained from standardized antenatal care and delivery files. Results: There weere no differences concerning gestational age, mode of delivery or neonatal health between the two groups. The IVF couples were more stable over time - from pregnancy until the child was 1 year old - in their relationship, while the control group experienced a decrease in marital satisfaction. The children in the IVF group were assessed by their parents as being more regular/habitual, sensitive and manageable than the control children. Conclusion: The differences present between the groups were in favour of the IVF families, and the effects of the infertility crisis were not notable when the children were 1 year old.
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| 8. |
- Sydsjö, Gunilla, 1956-, et al.
(författare)
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Relationships in couples after failed IVF treatment : A prospective follow-up study
- 2005
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Ingår i: Human Reproduction. - : Oxford University Press (OUP). - 0268-1161 .- 1460-2350. ; 20:7, s. 1952-1957
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Tidskriftsartikel (refereegranskat)abstract
- Background: There are few studies of couples that analyse satisfaction with treatment, adoption plans and relationships in couples after unsuccessful IVF. Methods: ENRICH marital inventory was used to describe marital dynamics and to gain information about treatment and adoption plans. A specially designed questionnaire was used. Of the 51 couples without previous children who were asked to participate after their first failed IVF cycle, 45 participated. The next stage of the study was carried out when the couples had reached the 6 months point after the first IVF cycle, and the last stage after the couples had been through one to three treatments, 1 1/2 years after the last treatment. Results: The couples displayed a stable relationship from the start as well as 1 year after the last IVF cycle. The vast majority of the couples had decided to go through with an adoption. Seventy-three percent of the women were interested in more IVF treatment compared to 33% of the men. Conclusion: The stresses associated with IVF treatment did not have a negative impact on the couples' appreciation of their relationships during and after the treatment period. After treatment had been completed, the couples seemed to have reoriented themselves toward other solutions to childlessness.
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