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- Kolakowska, J., et al.
(författare)
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Progress and challenges in the proteomics of domestic pig in research on the female reproductive system
- 2016
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Ingår i: Journal of Elementology. - : Polish Society for Magnesium Research. - 1644-2296. ; 21:4, s. 1055-1069
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Tidskriftsartikel (refereegranskat)abstract
- The proteomics of pigs is developing dynamically, attracting much attention among representatives of medical and health sciences, veterinary medicine, agricultural and natural sciences. The pig has been widely studied in genetics and genomics. However, proteomic applications are still awaiting more extensive implementation, both in the use of pigs as animal models in biomedicine sciences, and in the exploration of physiological tracts important for pig production. Underdeveloped databases for identification and analysis of porcine proteins as well as the scarcity of detailed information on similarities and differences between humans and pigs at the molecular level are hampering the pig proteomics. However, the use of pigs in proteomic studies in both physiological and biomedical sciences is prevalent comparing to other farm animals. The focus of the reported pig model proteomics studies is on exploring physiology and diseases, and on improving pig breading and productivity. This species has been used as a model in proteomics studies involved in ocular, brain, nutritional and reproduction research, etc. In the present paper we discuss technologies and bioinformatic tools used in studies of a proteome to verify the peptide- and protein-based content and we summarize the current status of proteomic studies of pigs. We focus on studies of the female reproductive system because the examination and understanding of the biology of oocytes, the oviduct and the uterus could facilitate the identification of mechanisms involved in the prenatal development, and it may help to develop new treatment for infertility of farm animals. © 2016, Polish Society Magnesium Research. All rights reserved.
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| 2. |
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| 3. |
- Kolakowska, J., et al.
(författare)
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Proteomic analysis of the endometrium during early pregnancy in the domestic pig
- 2017
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Ingår i: Reproduction Fertility and Development. - : CSIRO Publishing. - 1031-3613. ; 29:11, s. 2255-2268
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Tidskriftsartikel (refereegranskat)abstract
- Reproductive processes in domestic pigs have been studied extensively. Pigs are one of the main sources of meat for human consumption and are an established model for investigations into mammalian, including human, reproductive physiology. Studies of the uterus during early pregnancy will lead to a better understanding of mechanisms governing pregnancy. Proteomics provides the possibility to explore endometrial functions in an unbiased way. The aim of the study was to compare endometrium harvested from Days 12-13 and 15-16 of pregnancy with the corresponding days of the oestrous cycle. We identified endometrial proteins that are unique to the early stages of pregnancy (Days 12-13 and 15-16). Twenty-one proteins were identified that were uniquely expressed on the selected days of pregnancy or the oestrous cycle. Out of 21 identified proteins, 14 referred to the pregnancy periods. Systemic analysis of the identified proteins revealed cell adhesion and cytoskeletal organisation as two of the major functions, both of which are important for the establishment and maintenance of pregnancy. Thrombospondin 1 expression was validated using western blotting analysis and the results suggest its involvement in the adhesiveness of the embryo during the peri-implantation period in pigs.
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| 4. |
- Saini, Ravi Kanth Rao, et al.
(författare)
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Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells
- 2014
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Ingår i: Biochemical and Biophysical Research Communications. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 454:1, s. 202-209
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Tidskriftsartikel (refereegranskat)abstract
- Neuroblastoma develops through processes which include cellular dedifferentiation. Ability of tumors to form spheroids is one of the manifestations of dedifferentiation and carcinogenic transformation. To study mechanisms of dedifferentiation of neuroblastoma cells, we generated spheroids and performed a proteomics study to compare the spheroids with parental SK-N-BE2 cells. We observed that dedifferentiation induced extensive changes in the proteome profiles of the cells, which affected more than 30% of detected cellular proteins. Using mass spectrometry, we identified 239 proteins affected by dedifferentiation into spheroids as compared to the parental cells. These proteins represented such regulatory processes as transcription, cell cycle regulation, apoptosis, cell adhesion, metabolism, intracellular transport, stress response, and angiogenesis. A number of potent regulators of stemness, differentiation and cancer were detected as subnetworks formed by the identified proteins. Our validation tissue microarray study of 30 neuroblastoma cases confirmed that two of the identified proteins, DISC1 and DNA-PKcs, had their expression increased in advanced malignancies. Thus, our report unveiled extensive changes of the cellular proteome upon dedifferentiation of neuroblastoma cells, indicated top subnetworks and clusters of molecular mechanisms involved in dedifferentiation, and provided candidate biomarkers for clinical studies. (C) 2014 Elsevier Inc. All rights reserved.
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