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| 2. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
- Geidne, Susanna, Docent, 1975-, et al.
(författare)
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Health Promotion Interventions in Sports Clubs: Can We Talk About a Setting-Based Approach? : A Systematic Mapping Review
- 2019
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Ingår i: Health Education & Behavior. - : Sage Publications. - 1090-1981 .- 1552-6127. ; 46:4, s. 592-601
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Forskningsöversikt (refereegranskat)abstract
- Many researchers and authorities have recognized the important role that sports clubs can play in public health. In spite of attempts to create a theoretical framework in the early 2000s, a thorough understanding of sports clubs as a setting for health promotion (HP) is lacking. Despite calls for more effective, sustainable, and theoretically grounded interventions, previous literature reviews have identified no controlled studies assessing HP interventions in sports clubs. This systematic mapping review details how the settings-based approach is applied through HP interventions in sports clubs and highlights facilitators and barriers for sports clubs to become health-promoting settings. In addition, the mapped facilitators and barriers have been used to reformulate previous guidelines of HP in sports clubs. Seven databases were searched for empirical research published between 1986 and 2017. Fifty-eight studies were included, principally coming from Australia and Europe, describing 33 unique interventions, which targeted mostly male participants in team sports. The settings-based approach was not yet applied in sports clubs, as more than half of the interventions implemented in sports club targeted only one level of the socio-ecological model, as well as focused only on study participants rather than the club overall. Based on empirical data, the analysis of facilitators and barriers helped develop revised guidelines for sports clubs to implement settings-based HP. This will be particularly useful when implementing HP initiatives to aid in the development of sports clubs working with a whole setting approach.
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| 4. |
- Hamari, Lotta, et al.
(författare)
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Association of Self-Perceived Physical Competence and Leisure-Time Physical Activity in Childhood : A Follow-Up Study
- 2017
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Ingår i: Journal of School Health. - : John Wiley & Sons. - 0022-4391 .- 1746-1561. ; 87:4, s. 236-243
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUNDThe basis of self-perceived physical competence is built in childhood and school personnel have an important role in this developmental process. We investigated the association between initial self-perceived physical competence and reported leisure-time physical activity (LTPA) longitudinally in 10-, 12-, and 15-year-old children.METHODSThis longitudinal follow-up study comprises pupils from an elementary school cohort (N = 1346) in the city of Turku, Finland (175,000 inhabitants). The self-perceived physical competence (fitness and appearance) and LTPA data were collected with questionnaires. The full longitudinal data were available from 571 pupils based on repeated studies at the ages of 10, 12, and 15 years in 2004, 2006, and 2010. We analyzed the association of self-perceived physical competence and LTPA using regression models.RESULTSSelf-perceived physical competence was positively associated with LTPA at all ages (10 years p < .05, 12 years p < .0001, 15 years p < .0001). Increase in the self-perceived physical fitness scores was likely to associate with higher LTPA at each age point (10 years [odds ratio, OR] = 1.18, 95% confidence interval, CI: 1.09-1.27; 12 years [OR] = 1.27, 95% CI: 1.18-1.37; and 15 years [OR] = 1.28, 95% CI: 1.19-1.38).CONCLUSIONSSelf-perceived physical competence is associated with LTPA in children and adolescents, and the association is strengthened with age.
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| 5. |
- Kokko, Sami, et al.
(författare)
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Does sport club participation contribute to physical activity among children and adolescents in Finland : Sports Club for Health project
- 2017
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Konferensbidrag (refereegranskat)abstract
- Sports Club for Health (SCforH) is an EU-funded project that aims to promote physical acitivity through sports participation. SCforH has 6 different work packages, of which one is focusing on strengthening the scientific evidence base for the SCforH programs. In this work package, the aim is to compare the physical activity of youth who participate to sport club activities and those who don’t in different European countries. This abstract represents the Finnish data as an example.The Finnish is the National physical activity behaviour of children and adolescents (LIITU 2014) study. The nationally representative data was gathered through an internet survey in the spring 2014. In total 3071 children and adolescents aged 11yr (n=916), 13yr (n=935) and 15yr (n=951) provided responses for LIITU 2014. The variables measured were sport club (SC) participation and moderate to vigorous physical activity (MVPA).60 percent of Finnish youth participate to SC activities, with no differences between genders. 28 percent of youth in Finland met the PA recommendation of at least 60 minutes MVPA daily during last seven days. 34 percent of boys and 23 percent of girls met the recommendation (p<0.001). SC participants met the recommen- dation of MVPA more often than non-participants (p<0.001): 35 percent of those youth who did participate to SC activities and 18 percent of non-participants met the recommendation. The differences occurred also among genders: 41percent of SC participating boys met the recommendation, whereas 22 percent of non-participating boys did the same (p<0.001). Among girls the percentages were lower; 29 percent of SC participants and 15 percent of non-participants met the recommendation over past seven days (p<0.001).SC participants had higher percentages of meeting the MVPA guideline than non-participants. This highlights the role of sports clubs on recruiting different youth, preventing the drop-out and promoting the PA among adolescents.
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| 6. |
- Kokko, Sami, et al.
(författare)
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Does sports club participation contribute to physical activity among children and adolescents? A comparison across six European countries
- 2019
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Ingår i: Scandinavian Journal of Public Health. - : Sage Publications. - 1403-4948 .- 1651-1905. ; 47:8, s. 851-858
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Tidskriftsartikel (refereegranskat)abstract
- AIMS: Insufficient physical activity (PA) is one of the largest public health challenges of our time and requires a multisectoral public-health response. PA recommendations state that all children and adolescents should accumulate at least 60 minutes of moderate-to-vigorous PA (MVPA) daily and carry out vigorous PA (VPA) three times weekly. While participation in sports club activities is known to enhance the probability of reaching the recommended overall PA level, less is known about the contribution of sports club participation to VPA, and few cross-national comparisons have been carried out. The purpose of this paper is to study whether participation in sports club activities is associated with meeting the overall PA and VPA recommendations among children and adolescents across six European countries, namely Belgium (Flanders), Czech Republic, Finland, France, Ireland and Sweden.METHODS: Analyses were carried out on existing self-reported national data sets using descriptive statistics and logistic regression.RESULTS: Results indicate that approximately two-thirds of children and adolescents take part in sports club activities in the given countries. Sports club participants were more likely to meet the overall PA recommendations (OR 2.4-6.4) and VPA recommendation (OR 2.8-5.0) than non-participants.CONCLUSIONS: The extent to which overall PA and/or VPA is gained through sports club participation versus other settings needs to be further studied. Nonetheless, it can be argued that sports clubs have an important position in PA promotion for younger populations.
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