| 1. |
- Aerts, Joel, et al.
(författare)
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Guidance on current good radiopharmacy practice for the small-scale preparation of radiopharmaceuticals using automated modules : a European perspective
- 2014
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Ingår i: Journal of labelled compounds & radiopharmaceuticals. - : Wiley-Blackwell. - 0362-4803 .- 1099-1344. ; 57:10, s. 615-620
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Tidskriftsartikel (refereegranskat)abstract
- This document is meant to complement Part B of the EANM Guidelines on current good radiopharmacy practice (cGRPP) in the preparation of radiopharmaceuticals issued by the Radiopharmacy Committee of the European Association of Nuclear Medicine, covering small-scale in-house preparation of radiopharmaceuticals with automated modules. The aim is to provide more detailed and practice-oriented guidance to those who are involved in the small-scale preparation of radiopharmaceuticals, which are not intended for commercial purposes or distribution.
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| 2. |
- Bar-Sever, Zvi, et al.
(författare)
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Guidelines on nuclear medicine imaging in neuroblastoma
- 2018
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Ingår i: European Journal of Nuclear Medicine and Molecular Imaging. - : SPRINGER. - 1619-7070 .- 1619-7089. ; 45:11, s. 2009-2024
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Tidskriftsartikel (refereegranskat)abstract
- Nuclear medicine has a central role in the diagnosis, staging, response assessment and long-term follow-up of neuroblastoma, the most common solid extracranial tumour in children. These EANM guidelines include updated information on I-123-mIBG, the most common study in nuclear medicine for the evaluation of neuroblastoma, and on PET/CT imaging with F-18-FDG, F-18-DOPA and Ga-68-DOTA peptides. These PET/CT studies are increasingly employed in clinical practice. Indications, advantages and limitations are presented along with recommendations on study protocols, interpretation of findings and reporting results.
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| 3. |
- Bernhardsson, Magnus, et al.
(författare)
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Shining dead bone-cause for cautious interpretation of [F-18]NaF PET scans
- 2018
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Ingår i: Acta Orthopaedica. - : Taylor & Francis. - 1745-3674 .- 1745-3682. ; 89:1, s. 124-127
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Tidskriftsartikel (refereegranskat)abstract
- Background and purpose — [18F]Fluoride ([18F]NaF) PET scan is frequently used for estimation of bone healing rate and extent in cases of bone allografting and fracture healing. Some authors claim that [18F]NaF uptake is a measure of osteoblastic activity, calcium metabolism, or bone turnover. Based on the known affinity of fluoride to hydroxyapatite, we challenged this view.Methods — 10 male rats received crushed, frozen allogeneic cortical bone fragments in a pouch in the abdominal wall on the right side, and hydroxyapatite granules on left side. [18F]NaF was injected intravenously after 7 days. 60 minutes later, the rats were killed and [18F]NaF uptake was visualized in a PET/CT scanner. Specimens were retrieved for micro CT and histology.Results — MicroCT and histology showed no signs of new bone at the implant sites. Still, the implants showed a very high [18F]NaF uptake, on a par with the most actively growing and remodeling sites around the knee joint.Interpretation — [18F]NaF binds with high affinity to dead bone and calcium phosphate materials. Hence, an [18F]NaF PET/CT scan does not allow for sound conclusions about new bone ingrowth into bone allograft, healing activity in long bone shaft fractures with necrotic fragments, or remodeling around calcium phosphate coated prostheses
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| 4. |
- Huang, Ya-Yao, et al.
(författare)
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A two‐center study for the quality control of [18F]FDG using FASTlab phosphate cassettes
- 2016
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Ingår i: Annals of Nuclear Medicine. - : Springer. - 0914-7187 .- 1864-6433. ; 30:8, s. 563-571
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Tidskriftsartikel (refereegranskat)abstract
- Objective: The GE FASTlab radiosynthesis module is routinely used for the production of [18F]FDG, utilizing the commercially available phosphate cassettes. Because of the observation of a white precipitate in the product vial before the product expiry time, we re-examined the quality of the produced [18F]FDG solution.Methods: Phosphate buffered [18F]FDG solution was synthesized on the FASTlab and analyzed at both National Taiwan University Hospital (NTUH) of Taiwan and Royal Brisbane and Women’s Hospital (RBWH) of Australia. In addition to the standard product quality control (QC), the concentration of aluminum (Al3+) as probable cause of the precipitations in the [18F]FDG solution was analyzed by inductively coupled plasma mass spectrometry (ICP-MS at RBWH) and inductively coupled plasma optical emission spectrometry (ICP-OES at NTUH), and using three semi-quantitative methods at NTUH, Advantec® Alumi Check Test Strip, Quantofix® Aluminum Test Strip and MColortest™ Aluminum Test kit.Results: The precipitates were observed in the [18F]FDG solution within 24 (NTUH) and 6 (RBWH) hours after the end of synthesis in 38–100 % of the batches, dependent on the batch of the FASTlab cassettes. Addition of metal-free HCl(aq) to aliquots of [18F]FDG containing precipitate, followed by ICP-MS analysis revealed Al3+ concentrations of 70–80 ppm. Al3+ concentrations of 10–12 ppm were detected in [18F]FDG batches that did not show any precipitation. In contrast, less than 5 ppm of the residual Al3+ was detected by semi-quantitative methods in all batches.Conclusion: The US (USP), British (BP), European (EP) and Japanese (JP) pharmacopeias demand that [18F]FDG for injection should be clear and particulate free within the given shelf-life/expiration time. To avoid Al-phosphate precipitation within the product expiry time, FASTlab citrate cassettes, rather than phosphate cassettes, should be used for [18F]FDG production. Although testing for Al3+ is not listed in the [18F]FDG monographs of the USP, BP and EP, residual Al3+ levels should be considered in the interests of patient safety.
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| 5. |
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| 6. |
- Koziorowski, Jacek
(författare)
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Radiohalogenation of biomolecules : An experimental study on radiohalogen preparation, precursor synthesis, radiolabeling and biodistribution
- 1998
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Radiohalogens are widely used in nuclear medicine, both as tool for diagnostic in vivo imaging and inradionuclide therapy. This study deals with the use of radiohalogens; separation, precursor synthesis, labeling and biological behavior. The focus is on 211At and 124I, the former being a candidate for nuclide therapy and the latter potentially useful for diagnostic imaging and Auger-electron based radiotherapy. For astatine the separation, labeling and some biological behavior is described, and for iodine the latter two.Astatine was separated from an irradiated bismuth target by dry distillation. A novel cryotrap wasdeveloped for the isolation of astatine and subsequent synthesis of radiolabeled compounds. 5-[211At]astato-2´-deoxyuridine (AUdR) and N-succinimidyl-4-[211At]astatobenzoate (SAB) were synthesized in ~95% respectively ~90% radiochemical yields. The former is incorporated into DNA of proliferating cells and can therefore be used as an endoradiotherapeutic agent. The latter is a conjugate for the astatination of proteins. Human epidermal growth factor (hEGF) was tagged with astatine using three approaches: a) direct labeling of native hEGF, b) conjugation with SAB, and c) direct labeling of an hEGF - 7-(3-aminopropyl)-7,8-dicarba-nido-undecaborate(l-) conjugate. The overall labeling yields were ~3.5% for direct labeling, ~44% for SAB and ~70% for the hEGF-nido-carborane conjugate.A new route to N-succinimidyl 3- and 4- [124I]iodobenzoate, two reagents for radioiodination of proteins is described affording 90% radiochemical yield. Three radioiodinated analogs of PK11195, 1-(2-chlorophenyl)-N-methyl-N-(l-methylpropyl)isoquinoline-3-carboxyamide, a peripheral-type benrodiazepine receptor antagonist, were synthesized. All three analogs were obtained in >90% radiochemical yield. Synthesis and application of 5-[124I]iodo-2´-deoxyuridine (IUdR) is presented.The close-dodecaborate anion was evaluated as prosthetic group for radioiodination of macromolecules. Its inertness to in vivo dehalogenation was demonstrated by low thyroidal radioiodine uptake in the rat (0.07% ID/g, 20 h post injection).
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| 7. |
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| 8. |
- Koziorowski, Jacek, et al.
(författare)
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Radiolabeled Nanoparticles for Cancer Diagnosis and Therapy
- 2017
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Ingår i: ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY. - : BENTHAM SCIENCE PUBL LTD. - 1871-5206. ; 17:3, s. 333-354
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Forskningsöversikt (refereegranskat)abstract
- Cancer remains as one of the major causes of death worldwide. The emergence of nanotechnology has opened new avenues for the development of nanoparticle (NP)-based diagnostic and therapeutic tools. NPs of different chemical composition, size, shape and surface decoration can be prepared using a wide variety of synthetic strategies. Subsequent radiolabelling with positron or gamma emitters results in potential diagnostic agents which may offer improved selectivity and/or specificity for the target organ or tissue, enabling the acquisition of images with higher signal-to-contrast ratio. Incorporation of alpha or beta emitters leads to therapeutic agents with application in the field of radiotherapy. Here, we first describe the different labeling strategies reported so far for the incorporation of radionuclides into NPs. Recent advances in the use of nanoparticulate constructs both in the diagnostic and therapeutic arenas are then discussed and examples of their application are briefly discussed.
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| 9. |
- Mihon, Mirela, et al.
(författare)
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INFLUENCE OF THE SEPARATION PARAMETERS APPLIED FOR DETERMINATION OF IMPURITIES FDG AND CLDG
- 2017
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Ingår i: Farmacia. - : SOC STINTE FARMACEUTICE ROMANIA. - 0014-8237 .- 2065-0019. ; 65:1, s. 153-158
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Tidskriftsartikel (refereegranskat)abstract
- 2-fluoro-2-deoxy-D-glucose (FDG) and 2-chloro-2-deoxy-D-glucose (CIDG) are chemical impurities found in the 2-[F-18]fluoro-2-deoxy-D-glucose products (F-18-FDG). The objective of this study was to find the best condition for the separation of FDG and CIDG, evaluating different columns under various operating conditions. Chromatographic parameters such as column temperature, composition and flow rate of the mobile phase were the independent variables used in the optimization process. The optimized method was validated and validation results showed a good accuracy, repeatability and reproducibility.
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| 10. |
- Sundin, Johanna, et al.
(författare)
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High yield direct 76Br-bromination of monoclonal antibodies using Cloramine-T
- 1999
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Ingår i: Nuclear Medicine and Biology. - 0969-8051 .- 1872-9614. ; 26:8, s. 923-929
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Tidskriftsartikel (refereegranskat)abstract
- Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean +/- max error) of 77 +/- 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean +/- max error) of 75 +/- 3%, 63 +/- 4%, and 73 +/- 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.
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