| 1. |
- Hedin, Karl Alex, et al.
(författare)
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Cold Exposure and Oral Delivery of GLP-1R Agonists by an Engineered Probiotic Yeast Strain Have Antiobesity Effects in Mice
- 2023
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Ingår i: ACS Synthetic Biology. - 2161-5063. ; 12:11, s. 3433-3442
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Tidskriftsartikel (refereegranskat)abstract
- Advanced microbiome therapeutics (AMTs) holds promise in utilizing engineered microbes such as bacteria or yeasts for innovative therapeutic applications, including the in situ delivery of therapeutic peptides. Glucagon-like peptide-1 receptor agonists, such as Exendin-4, have emerged as potential treatments for type 2 diabetes and obesity. However, current administration methods face challenges with patient adherence and low oral bioavailability. To address these limitations, researchers are exploring improved oral delivery methods for Exendin-4, including utilizing AMTs. This study engineered the probiotic yeast Saccharomyces boulardii to produce Exendin-4 (Sb-Exe4) in the gastrointestinal tract of male C57BL/6 mice to combat diet-induced obesity. The biological efficiency of Exendin-4 secreted by S. boulardii was analyzed ex vivo on isolated pancreatic islets, demonstrating induced insulin secretion. The in vivo characterization of Sb-Exe4 revealed that when combined with cold exposure (8 °C), the Sb-Exe4 yeast strain successfully suppressed appetite by 25% and promoted a 4-fold higher weight loss. This proof of concept highlights the potential of AMTs to genetically modify S. boulardii for delivering active therapeutic peptides in a precise and targeted manner. Although challenges in efficacy and regulatory approval persist, AMTs may provide a transformative platform for personalized medicine. Further research in AMTs, particularly focusing on probiotic yeasts such as S. boulardii, holds great potential for novel therapeutic possibilities and enhancing treatment outcomes in diverse metabolic disorders.
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| 2. |
- Johansson, Roger, et al.
(författare)
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Reading during text production
- 2012
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Ingår i: Current trends in European Writing Research. - Bingley, UK : Emerald. ; , s. 359-361, s. 359-361
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Bokkapitel (refereegranskat)
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| 3. |
- Johansson, Roger, et al.
(författare)
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Text production in handwriting versus computer typing
- 2012
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Ingår i: Current trends in European Writing Research. - Bingley, UK : Emerald Group Publishing Limited. - 1572-6304. ; , s. 375-377, s. 359-361
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Bokkapitel (refereegranskat)
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| 4. |
- Wengelin, Åsa, et al.
(författare)
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Investigating writing processes with keystroke logging
- 2024
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Ingår i: Digital Writing Technologies in Higher Education. - : Springer Cham. ; :1, s. 16
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Bokkapitel (populärvet., debatt m.m.)abstract
- Already in the 1970s, researchers in linguistics and psychology became interested in understanding how written language production worked, why students’ texts ended up in a specific way, and whether writing instruction could be improved by an increased understanding of students’ actual activities during writing - what happens “behind the scenes”. They observed writing processes through video- recordings and think-aloud protocols, both of which required laborious manual anal- yses, but with the advent of affordable computers in the 1990s keystroke logging was developed. Keystroke logging records all keystrokes and mouse movements and provide them with a time stamp to allow playback and analyses. The purpose of this chapter is to introduce the reader to the concept of keystroke logging, explain briefly how it works, and give an overview of currently available software. First, we provide a short historical background. We then move into the core idea and functionality of keystroke logging in general before turning to descriptions of specific pieces of software. We summarise similarities and differences, aiming to show that choice of software should be governed by the research question. Finally, we discuss research that uses keystroke logging as a research tool, and provide examples of research about keystroke logging as a pedagogical tool.
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| 5. |
- Wengelin, Åsa, 1968, et al.
(författare)
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Investigating Writing Processes with Keystroke Logging
- 2023
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Ingår i: Kruse, O., Rapp, C., Anson, C., Benetos, K., Cotos, E., Devitt, A., & Shibani, A. (Eds.) Digital Writing Technologies in Higher Education Theory, Research, and Practice. - Cham : Springer. - 9783031360350 ; , s. 405-420
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Bokkapitel (refereegranskat)
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