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| 2. |
- Carlsbecker, A, et al.
(författare)
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The DAL10 gene from Norway spruce (Picea abies) belongs to a potentially gymnosperm-specific subclass of MADS-box genes and is specifically active in seed cones and pollen cones.
- 2003
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Ingår i: Evolution & Development. - 1525-142X. ; 5:6, s. 551-561
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Tidskriftsartikel (refereegranskat)abstract
- Transcription factors encoded by different members of the MADS-box gene family have evolved central roles in the regulation of reproductive organ development in the flowering plants, the angiosperms. Development of the stamens and carpels, the pollen- and seed-bearing organs, involves the B- and C-organ-identity MADS-box genes. B- and C-type gene orthologs with activities specifically in developing pollen- and seed-bearing organs are also present in the distantly related gymnosperms: the conifers and the gneto-phytes. We now report on the characterization of DAL10, a novel MADS-box gene from the conifer Norway spruce, which unlike the B- and C-type conifer genes shows no distinct orthology relationship to any angiosperm gene or clade in phylogenetic analyses. Like the B- and C-type genes, it is active specifically in developing pollen cones and seed cones. In situ RNA localization experiments show DAL10 to be expressed in the cone axis, which carry the microsporophylls of the young pollen cone. In contrast, in the seed cone it is expressed both in the cone axis and in the bracts, which subtend the ovuliferous scales. Expression data and the phenotype of transgenic Arabidopsis plants expressing DAL10 suggest that the gene may act upstream to or in concert with the B- and C-type genes in the establishment of reproductive identity of developing cones.
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| 3. |
- Just, Kadri, et al.
(författare)
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Monitoring infection of tomato fruit by Tomato yellow leaf curl virus
- 2016
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Ingår i: Plant Pathology. - : Blackwell Publishing. - 0032-0862 .- 1365-3059.
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Tidskriftsartikel (refereegranskat)abstract
- Fruit constitutes a strong sink organ and thus accumulates infecting viruses, but there is limited information about the infection process of viruses in fruit. Tomato yellow leaf curl virus (TYLCV, genus Begomovirus, family Geminiviridae) is one of the most important viruses affecting the production of tomato fruit. Using real-time quantitative PCR, TYLCV was shown to accumulate with increasing titres in early developing tomato fruit tissues from anthesis until 21 days post-anthesis. In situ hybridization demonstrated that TYLCV DNA and transcripts of the coat protein gene localized specifically to the phloem tissue of young fruit as well as sepals and petals. Embryos of developing seeds were also found to be infected. Expression of a host histone H4 gene was used as a marker for S-phase and the gene was also found to be expressed in phloem cells of young tomato fruit. The results indicate that TYLCV is transported to developing tomato fruit, where the virus titre gradually increases because of movement and probably also due to virus replication. In this study, the accumulation and localization of TYLCV in early developing tomato fruit are monitored for the first time.
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| 4. |
- KVARNHEDEN, A, et al.
(författare)
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A CDC2 HOMOLOG AND CLOSELY-RELATED PROCESSED RETROPSEUDOGENES FROM NORWAY SPRUCE
- 1995
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Ingår i: PLANT MOLECULAR BIOLOGY. - : KLUWER ACADEMIC PUBL. - 0167-4412. ; 27:2, s. 391-403
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The p34(cdc2) protein kinase is a key component in the regulation of the eukaryotic cell cycle and has been conserved during evolution. We have isolated cDNA clones corresponding to a cdc2 gene (cdc2Pa) from the conifer Norway spruce, Picea abies (L.) Kar
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| 5. |
- Kvarnheden, A, et al.
(författare)
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Molecular evolution of cdc2 pseudogenes in spruce (Picea)
- 1998
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Ingår i: PLANT MOLECULAR BIOLOGY. - : KLUWER ACADEMIC PUBL. - 0167-4412. ; 36:5, s. 767-774
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The p34(cdc2) protein and other cyclin-dependent protein kinases (CDK) are important regulators of eukaryotic cell cycle progression. We have previously cloned a functional cdc2 gene from Picea abies and found it to be part of a family of related sequence
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