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Sökning: WFRF:(Ladero Miguel)

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1.
  • Cano, María Emilia, et al. (författare)
  • Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
  • 2024
  • Ingår i: International Journal of Biological Macromolecules. - : Elsevier B.V.. - 0141-8130 .- 1879-0003. ; 267
  • Tidskriftsartikel (refereegranskat)abstract
    • Biomass-derived oligo- and polysaccharides may act as elicitors, i.e., bioactive molecules that trigger plant immune responses. This is particularly important to increase the resistance of plants to abiotic and biotic stresses. In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsicum annuum). Based on the characterization of the gene expression with Next Generation Sequencing (NGS) of the C. annuum’s total messenger RNA, the differences in growth of the C. annuum seeds correlated well with the downregulation of the pathways regulating photosynthesis. A downregulation of the response to abiotic factors was detected, suggesting that these gels would improve the resistance of the C. annuum plants to abiotic stress due to, e.g., water deprivation and cold temperatures. 
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2.
  • Santos, Sara M., et al. (författare)
  • Use of bacterial cellulose in degraded paper restoration. Part I : application on model papers
  • 2016
  • Ingår i: Journal of Materials Science. - : Springer Science and Business Media LLC. - 0022-2461 .- 1573-4803. ; 51:3, s. 1541-1552
  • Tidskriftsartikel (refereegranskat)abstract
    • The disappearance of bibliographic heritage is one of the biggest problems facing libraries. One of the most common methods used to restore paper, lining, is to apply a reinforcing layer to the document. This study focuses on lining papers with bacterial cellulose (BC) sheets from Gluconacetobacter sucrofermentans. For this purpose, several model papers have been selected. They have been characterized before and after the lining with this BC and a specific Japanese paper (JP) to compare both materials. Taking into account the differences between bacterial and vegetal cellulose is expected that the results may be similar to other BC and JP. The samples have been characterized before and after an aging process. There are no significant differences in some of the characteristics studied. Nevertheless, BC-lined papers present higher gloss values and b* coordinate. The wettability decreases with both BC and JP. However, in papers lined with BC, the wettability decreases more markedly and independently of the model paper used. This is related to the sealing of the surface structure by BC, which also leads to a reduction of air permeability. When the lined papers go through an aging process, there are no significant changes in any characteristic, except in b* and L* color coordinates. Additionally, the wettability rate decreases in all cases. This study indicates that papers lined with BC are stable over time. Finally, the use of BC as reinforcing material may offer advantages for specific conservation treatments, being more suitable for certain types of paper than JP.
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3.
  • Santos, Sara M., et al. (författare)
  • Use of bacterial cellulose in degraded paper restoration. Part II : application on real samples
  • 2016
  • Ingår i: Journal of Materials Science. - : Springer Science and Business Media LLC. - 0022-2461 .- 1573-4803. ; 51:3, s. 1553-1561
  • Tidskriftsartikel (refereegranskat)abstract
    • Preservation of documentary heritage is one of the biggest challenges facing paper conservators today. The singular properties of bacterial cellulose (BC) lead us to propose to reinforce paper with BC sheets. In the first part of this study, the reinforcing capability of BC was tested on model papers of well-known fiber composition. The aim of the present study was to verify the suitability of rebuilding degraded old papers with BC. The degraded papers were characterized before and after the reinforcement. In addition, lined samples were characterized before and after an aging process in order to study the stability in time. The same methodology was used with Japanese paper (JP), a material commonly used by paper conservators, in order to compare both materials as reinforcement. Mechanical properties of paper lined with BC are as good as those obtained with JP. Papers lined with BC have more marked modifications on their optical properties than those restored with JP. Nevertheless, letters in books lined with BC are more legible. Moreover, only the papers restored with BC show high changes in porosity. The aging process leads to a slight decrement in burst index. Changes on tear index and optical properties with the aging process depend on the paper to be restored. This study suggests that BC improves deteriorated paper quality, without altering the information contained therein, and that this improvement is maintained over time. Hence, BC is a promising alternative material for the restoration of paper.
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