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- Landreh, Luise
(författare)
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Identification and characterization of stem Leydig cells in the testis
- 2014
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- In the testis, several cell types are needed to ensure male fertility, among them the testosterone-producing Leydig cells. Testosterone supports the maturation of sperm and maintains the male phenotype. Functional Leydig cells are therefore required to prevent testicular malfunction. Yet in contrast to their well-established role in the testis, the origin of the adult Leydig cell lineage has largely remained unclear. A potential reservoir for putative stem Leydig cells are the peritubular myoid cells, which show characteristics of pluripotency as well as steroidogenic capacity and can be isolated to study the differentiation mechanisms of steroidogenic cells. The scope of this work is to elucidate cells and pathways involved in the underlying processes. Common to all stages of Leydig cell development is the presence of the platelet-derived growth factor receptor alpha (PDGFRα). Stem Leydig cells have been described to be PDGFRα-positive and to not express the luteinizing hormone receptor (LHR), a classical marker for differentiated Leydig cells. To identify the stem Leydig cell in the testis, we first analyzed the two steroidogenic cell types in the postnatal rodent testis. Both, the fetal Leydig cells and the peritubular cells were PDGFRα positive and showed a transient capacity to produce steroids. Stimulation of the cAMP-PKA pathway increased the steroidogenic potential in both cell types. However, while the FLCs were responsive to hCG stimulation, the PTCs expressed no LHR and their steroid production did not increase during hCG treatment. The isolated PDGFRα-positive, LHR-negative rat PTCs expressed markers for pluripotent, steroidogenic and myoid cells, indicating that they include putative stem Leydig cells. Subsequently, we examined the transferability of the rodent data into humans. We found that the peritubular cells in the adult human testis (HTPC) also expressed the PDGFRα and showed characteristics of pluripotent as well as steroidogenic cells. Stimulation of the cAMP-PKA pathway in isolated HTPCs increased the steroid production, underscoring the similarities between rat and human Leydig cell development. To be able to study early differentiation mechanisms in detail, the rapidly developing field of stem cell technology opens new possibilities. As the overexpression of the steroidogenic factor 1 (SF-1) has been described to induce the differentiation of steroidogenic cells from mesenchymal and embryonic stem cells, we characterized seven human embryonic stem cell lines with the aim of identifying similar factors. We found that all seven cell lines express different marker profiles for differentiating cells, with one cell line, i.e. HS361 showing characteristics of Sertoli-like cells and another cell line, i.e.HS420, expressing early differentiation marker for steroidogenic cells. Further characterization and differentiation of this cell line could increase our understanding of the development of steroidogenic cells. In summary, this work shows that the peritubular cell population in the testis contains putative stem Leydig cells and that these cells can be stimulated to become steroidogenic. The cAMP-PKA pathway plays an important role in the maintenance of steroidogenesis and prevents dedifferentiation under in vitro conditions. We furthermore identified candidate human embryonic stem cell lines for the study of the underlying mechanisms of gonadal cell development.
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| 2. |
- Shabalina, Irina G., et al.
(författare)
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Leydig cell steroidogenesis unexpectedly escapes mitochondrial dysfunction in prematurely aging mice
- 2015
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Ingår i: The FASEB Journal. - : Wiley. - 0892-6638 .- 1530-6860. ; 29:8, s. 3274-3286
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Tidskriftsartikel (refereegranskat)abstract
- Point mutations and deletions of mitochondrial DNA (mtDNA) accumulate in tissues during aging in animals and humans and are the basis for mitochondrial diseases. Testosterone synthesis occurs in the mitochondria of Leydig cells. Mitochondrial dysfunction (as induced here experimentally in mtDNA mutator mice that carry a proofreading-deficient form of mtDNA polymerase gamma, leading to mitochondrial dysfunction in all cells types so far studied) would therefore be expected to lead to low testosterone levels. Although mtDNA mutator mice showed a dramatic reduction in testicle weight (only 15% remaining) and similar decreases in number of spermatozoa, testosterone levels in mt DNA mutator mice were unexpectedly fully unchanged. Leydig cell did not escape mitochondrial damage (only 20% of complex I and complex IV remaining) and did show high levels of reactive oxygen species (ROS) production (>5-fold increased), and permeabilized cells demonstrated absence of normal mitochondrial function. Nevertheless, within intact cells, mitochondrial membrane potential remained high, and testosterone production was maintained. This implies development of a compensatory mechanism. A rescuing mechanism involving electronsfrom the pentose phosphate pathway transferred via a 3-fold up-regulated cytochrome b5 to cytochrome c, allowing for mitochondrial energization, is suggested. Thus, the Leydig cells escape mitochondrial dysfunction via a unique rescue pathway. Such a pathway, bypassing respiratory chain dysfunction, may be of relevance with regard to mitochondrial disease therapy and to managing ageing in general.
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