| 1. |
- Garcia-Ryde, Martin, et al.
(författare)
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Lung Fibroblasts from Chronic Obstructive Pulmonary Disease Subjects Have a Deficient Gene Expression Response to Cigarette Smoke Extract Compared to Healthy
- 2023
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Ingår i: International journal of chronic obstructive pulmonary disease. - 1178-2005. ; 18, s. 2999-3014
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND AND AIM: Cigarette smoking is the most common cause of chronic obstructive pulmonary disease (COPD) but more mechanistic studies are needed. Cigarette smoke extract (CSE) can elicit a strong response in many COPD-related cell types, but no studies have been performed in lung fibroblasts. Therefore, we aimed to investigate the effect of CSE on gene expression in lung fibroblasts from healthy and COPD subjects.PATIENTS AND METHODS: Primary lung fibroblasts, derived from six healthy and six COPD subjects (all current or ex-smokers), were either unstimulated (baseline) or stimulated with 30% CSE for 4 h prior to RNA isolation. The mRNA expression levels were measured using the NanoString nCounter Human Fibrosis V2 panel (760 genes). Pathway enrichment was assessed for unique gene ontology terms of healthy and COPD.RESULTS: At baseline, a difference in the expression of 17 genes was found in healthy and COPD subjects. Differential expression of genes after CSE stimulation resulted in significantly less changes in COPD lung fibroblasts (70 genes) than in healthy (207 genes), with 51 genes changed in both. COPD maintained low NOTCH signaling throughout and upregulated JUN >80%, indicating an increase in apoptosis. Healthy downregulated the Mitogen-activated protein kinase (MAPK) signaling cascade, including a ≥50% reduction in FGF2, CRK, TGFBR1 and MEF2A. Healthy also downregulated KAT6A and genes related to cell proliferation, all together indicating possible cell senescence signaling.CONCLUSION: Overall, COPD lung fibroblasts responded to CSE stimulation with a very different and deficient expression profile compared to healthy. Highlighting that stimulated healthy cells are not an appropriate substitute for COPD cells which is important when investigating the mechanisms of COPD.
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| 2. |
- Korneeva, E.V., et al.
(författare)
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Crystalline nickel(II) di-i-amyl dithiophosphate, [Ni{S2P(O-i-C5H11)2}2] : Preparation, structure, heteronuclear (13C, 31P) CP/MAS NMR spectra, and thermal behavior
- 2017
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Ingår i: Russian journal of coordination chemistry. - : Springer. - 1070-3284 .- 1608-3318. ; 43:4, s. 223-231
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Tidskriftsartikel (refereegranskat)abstract
- The crystalline nickel(II) di-i-amyl) dithiophosphate (Dtph), [Ni{S2P(O-i-C5H11)2}2] (I) was isolated on a preparative scale and characterized by 13C, 31P MAS NMR, and X-ray diffraction (CIF file CCDC no. 1469369). The χ2-statistic diagrams were constructed from full 31P CP/MAS NMR spectra for calculating the 31P chemical shift anisotropy: δaniso = δzz–δiso and the asymmetry parameter η = (δyy–δxx)/(δzz–δiso). The key structural unit of I is the centrosymmetric [Ni{S2P(O-i-C5H11)2}2] molecule in which the nickel atom coordinates two Dtph ligands in the isobidentate fashion. In molecule I, each carbon, oxygen, and sulfur atom is statistically disordered over two sites with equal occupancies. However, the disorder does not affect nickel and phosphorus. These results were interpreted as the presence in I of two [Ni{S2P(O-i-C5H11)2}2] molecules rotated through 21.0(1)° (the angle between the [NiS4] chromophore planes) relative to each other around the bisecting P–Ni–P axis passing through both four-membered [NiS2P] rings. The two molecules occupy crystal lattice sites with equal probabilities. The thermal behavior of I was studied by simultaneous thermal analysis under argon. The course of the thermal destruction of the complex was established, nickel pyrophosphate (Ni2P2O7) was identified as the final product of thermal transformations.
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| 3. |
- Ahlström, Torbjörn, et al.
(författare)
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Genom livet i den långsamma filen
- 2005
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Ingår i: Arkeologi och naturvetenskap : Gyllenstiernska Krapperupstiftelsens Symposium Nr 6 År 2003 - Gyllenstiernska Krapperupstiftelsens Symposium Nr 6 År 2003. - 1100-7095. - 9187944146 ; 6, s. 215-234
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 4. |
- Ivanov, Alexander V., et al.
(författare)
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Binding of Au3+ from solutions by nickel(II) and cadmium diisopropyl dithiophosphates: MAS P-31 NMR, structure and thermal behavior of the polynuclear complex [Au-2{S2P(O-iso-C3H7)(2)}(2)] (n)
- 2012
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Ingår i: Russian journal of coordination chemistry. - 1070-3284 .- 1608-3318. ; 38:6, s. 430-439
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Tidskriftsartikel (refereegranskat)abstract
- The paper deals with reactions of freshly precipitated diisopropyl dithiophosphate (Dtph) complexes of nickel(II), [Ni{S2P(O-iso-C3H7)(2)}(2)] and cadmium, [Cd-2{S2P(O-iso-C3H7)(2)}(4)], with the [AuCl4](-) in 2M HCl, resulting in gold transition from the solution to the precipitate as polymeric gold(I) diisopropyl dithiophosphate. The reduction of gold(III) to gold(I) noted in both cases is due to oxidation of the relevant part of the Dtph group to bis(O,O'-di-(iso)-propoxythiophosphoryl) disulfide, (iso-C3H7O)(2)P(S)S-S(S)P(O-iso-C3H7)(2). The polynuclear gold(I) complex [Au-2{S2P(O-iso-C3H7)(2)}(2)] (n) (I) was isolated on a preparative scale from the chemisorption system and studied by MAS P-31 NMR and X-ray diffraction. The key structural unit of I is the non-centrosymmetric binuclear molecule [Au-2{S2P(O-iso-C3H7)(2)}(2)] in which the gold atoms are connected by two bridging Dtph groups. The structure contains two types of non-equivalent binuclear molecules related as conformational isomers. Owing to the relatively weak Au-Au contacts, the neighboring binuclear [Au-2{S2P(O-iso-C3H7)(2)}(2)] molecules are involved in an infinite polymeric chain with conformer alternation along the chain. To elucidate the conditions for the recovery of bound gold(I), the precipitates formed in the sorption systems were studied by simultaneous thermal analysis under argon. As the final product, thermolysis gives reduced metallic gold. The ability of dithiophosphate complexes to bind gold from a solution is much lower than that of dithiocarbamate complexes, which is due to oxidation of some Dtph groups to disulfide.
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| 5. |
- Korneeva, E V, et al.
(författare)
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Polymeric Gold(I) Diisobutyl Dithiophosphate, [Au2{S2P(O-iso-C4H9)2}2]n: Synthesis, Supramolecular Self-Organisation (a Role of Aurophilic Interaction), 13C and 31P MAS NMR Spectroscopy, and Thermal Behavior
- 2014
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Ingår i: Russian journal of coordination chemistry. - 1070-3284 .- 1608-3318. ; 40:10, s. 748-756
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Tidskriftsartikel (refereegranskat)abstract
- A new polymeric gold(I) diisobutyl dithiophosphate (Dtph), [Au2{S2P(O-iso-C4H9)2}2] n (I), was preparatively obtained and characterized by 13C and 31P MAS NMR spectroscopy and X-ray diffraction (CIF file CCDC no. 977818). Diagrams of the χ2 statistic were constructed from the complete 31P MAS NMR spectra and used to calculate the 31P chemical shift anisotropy (δ aniso = δ zz − δ iso ) and the asymmetry parameter η = (δ yy − δ xx )/(δ zz − δ iso ). The main structural unit of complex I is the noncentrosymmetric dinuclear molecule [Au2{S2P(O-iso-C4H9)2}2], in which the gold atoms are linked by two bridging ligands Dtph. The central cyclic structural fragment of the dimer [Au2S4P2] is additionally stabilized by the intramolecular aurophilic interaction Au⋯Au. Further supramolecular self-organization of the complex involves intermolecular aurophilic bonds Au⋯Au that serve to unite adjacent dinuclear molecules [Au2{S2P(O-iso-C4H9)2}2] with different spatial orientations into the polymer chains ([Au2{S2P(O-iso-C4H9)2}2]) n . The thermal behavior of complex I was examined by synchronous thermal analysis under argon. The character of the thermolysis of the complex to reduced metallic gold as a final product was determined.
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| 6. |
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